Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks
The control of ticks through vaccination offers a sustainable alternative to the use of chemicals that cause contamination and the selection of resistant tick strains. However, only a limited number of anti-tick vaccines have reached commercial realization. In this sense, an antigen effective agains...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/11/1365 |
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| author | Alina Rodríguez-Mallon Pedro E. Encinosa Guzmán Yamil Bello Ana Domingos Sandra Antunes Petr Kopacek Ana Sofia Santos Rita Velez Jan Perner Frank L. Ledesma Bravo Helena Frantova Jan Erhart Rafmary Rodríguez Alier Fuentes David Diago Marisdania Joglar Luis Méndez Mario Pablo Estrada |
| author_facet | Alina Rodríguez-Mallon Pedro E. Encinosa Guzmán Yamil Bello Ana Domingos Sandra Antunes Petr Kopacek Ana Sofia Santos Rita Velez Jan Perner Frank L. Ledesma Bravo Helena Frantova Jan Erhart Rafmary Rodríguez Alier Fuentes David Diago Marisdania Joglar Luis Méndez Mario Pablo Estrada |
| author_sort | Alina Rodríguez-Mallon |
| collection | DOAJ |
| description | The control of ticks through vaccination offers a sustainable alternative to the use of chemicals that cause contamination and the selection of resistant tick strains. However, only a limited number of anti-tick vaccines have reached commercial realization. In this sense, an antigen effective against different tick species is a desirable target for developing such vaccines. A peptide derived from the tick P0 protein (pP0) conjugated to a carrier protein has been demonstrated to be effective against the <i>Rhipicephalus microplus, Rhipicephalus sanguineus</i>, and <i>Amblyomma mixtum</i> tick species. The aim of this work was to assess the efficacy of this peptide when conjugated to the Bm86 protein against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> ticks. An RNAi experiment using P0 dsRNA from <i>I. ricinus</i> showed a dramatic reduction in the feeding of injected female ticks on guinea pigs. In the follow-up vaccination experiments, rabbits were immunized with the pP0-Bm86 conjugate and challenged simultaneously with larvae, nymphs, and the adults of <i>I. ricinus</i> ticks. In the same way, horses were immunized with the pP0-Bm86 conjugate and challenged with <i>D. nitens</i> larva. The pP0-Bm86 conjugate showed efficacies of 63% and 55% against <i>I. ricinus</i> and <i>D. nitens</i> ticks, respectively. These results, combined with previous reports of efficacy for this conjugate, show the promising potential for its development as a broad-spectrum anti-tick vaccine. |
| first_indexed | 2024-03-09T16:32:28Z |
| format | Article |
| id | doaj.art-9548003d7a8f41cdad4507adfe28ba50 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T16:32:28Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-9548003d7a8f41cdad4507adfe28ba502023-11-24T14:59:55ZengMDPI AGPathogens2076-08172023-11-011211136510.3390/pathogens12111365Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> TicksAlina Rodríguez-Mallon0Pedro E. Encinosa Guzmán1Yamil Bello2Ana Domingos3Sandra Antunes4Petr Kopacek5Ana Sofia Santos6Rita Velez7Jan Perner8Frank L. Ledesma Bravo9Helena Frantova10Jan Erhart11Rafmary Rodríguez12Alier Fuentes13David Diago14Marisdania Joglar15Luis Méndez16Mario Pablo Estrada17Center for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, PortugalGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Rua da Junqueira 100, 1349-008 Lisboa, PortugalInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 České Budějovice, Czech RepublicCentro de Estudos de Vetores e Doenças Infeciosas Dr. Francisco Cambournac, Instituto Nacional de Saúde Doutor Ricardo Jorge (CEVDI-INSA), 2965-575 Águas de Moura, PortugalCentro de Estudos de Vetores e Doenças Infeciosas Dr. Francisco Cambournac, Instituto Nacional de Saúde Doutor Ricardo Jorge (CEVDI-INSA), 2965-575 Águas de Moura, PortugalInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 České Budějovice, Czech RepublicCenter for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 České Budějovice, Czech RepublicInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 České Budějovice, Czech RepublicNational Laboratory of Parasitology, Avenue San Antonio-Rincón, Km 1 1/2, Artemisa 32500, CubaNational Laboratory of Parasitology, Avenue San Antonio-Rincón, Km 1 1/2, Artemisa 32500, CubaCenter for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaNational Laboratory of Parasitology, Avenue San Antonio-Rincón, Km 1 1/2, Artemisa 32500, CubaCenter for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, CubaThe control of ticks through vaccination offers a sustainable alternative to the use of chemicals that cause contamination and the selection of resistant tick strains. However, only a limited number of anti-tick vaccines have reached commercial realization. In this sense, an antigen effective against different tick species is a desirable target for developing such vaccines. A peptide derived from the tick P0 protein (pP0) conjugated to a carrier protein has been demonstrated to be effective against the <i>Rhipicephalus microplus, Rhipicephalus sanguineus</i>, and <i>Amblyomma mixtum</i> tick species. The aim of this work was to assess the efficacy of this peptide when conjugated to the Bm86 protein against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> ticks. An RNAi experiment using P0 dsRNA from <i>I. ricinus</i> showed a dramatic reduction in the feeding of injected female ticks on guinea pigs. In the follow-up vaccination experiments, rabbits were immunized with the pP0-Bm86 conjugate and challenged simultaneously with larvae, nymphs, and the adults of <i>I. ricinus</i> ticks. In the same way, horses were immunized with the pP0-Bm86 conjugate and challenged with <i>D. nitens</i> larva. The pP0-Bm86 conjugate showed efficacies of 63% and 55% against <i>I. ricinus</i> and <i>D. nitens</i> ticks, respectively. These results, combined with previous reports of efficacy for this conjugate, show the promising potential for its development as a broad-spectrum anti-tick vaccine.https://www.mdpi.com/2076-0817/12/11/1365ticksP0 proteinanti-tick vaccinetick controlvaccination |
| spellingShingle | Alina Rodríguez-Mallon Pedro E. Encinosa Guzmán Yamil Bello Ana Domingos Sandra Antunes Petr Kopacek Ana Sofia Santos Rita Velez Jan Perner Frank L. Ledesma Bravo Helena Frantova Jan Erhart Rafmary Rodríguez Alier Fuentes David Diago Marisdania Joglar Luis Méndez Mario Pablo Estrada Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks Pathogens ticks P0 protein anti-tick vaccine tick control vaccination |
| title | Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks |
| title_full | Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks |
| title_fullStr | Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks |
| title_full_unstemmed | Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks |
| title_short | Efficacy of the Vaccine Candidate Based on the P0 Peptide against <i>Dermacentor nitens</i> and <i>Ixodes ricinus</i> Ticks |
| title_sort | efficacy of the vaccine candidate based on the p0 peptide against i dermacentor nitens i and i ixodes ricinus i ticks |
| topic | ticks P0 protein anti-tick vaccine tick control vaccination |
| url | https://www.mdpi.com/2076-0817/12/11/1365 |
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