Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads
Context: Biosurfactants are amphipathic molecules that reduce surface tension. The major reasons to economically production of the biosurfactant are their health, safety, environmental management, and promising applications. Aims: To optimize the immobilization of Pseudomonas aeruginosa as a bios...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2019-11-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol7/jppres19.628_7.6.413.pdf |
| _version_ | 1823995478909386752 |
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| author | Gholamreza Dehghannoudeh Kioomars Kiani Mohammad Hassan Moshafi Negar Dehghannoudeh Majid Rajaee Soodeh Salarpour Mandana Ohadi |
| author_facet | Gholamreza Dehghannoudeh Kioomars Kiani Mohammad Hassan Moshafi Negar Dehghannoudeh Majid Rajaee Soodeh Salarpour Mandana Ohadi |
| author_sort | Gholamreza Dehghannoudeh |
| collection | DOAJ |
| description | Context: Biosurfactants are amphipathic molecules that reduce surface tension. The major reasons to economically production of the biosurfactant are their health, safety, environmental management, and promising applications.
Aims: To optimize the immobilization of Pseudomonas aeruginosa as a biosurfactant producer in alginate beads.
Methods: Biosurfactant production by P. aeruginosa was confirmed through the hemolysis test, emulsification index, and surface activity measurement. Calcium alginate encapsulation technique was used in order to entrap the P. aeruginosa cells. Full factorial design was employed to optimize bead preparation. Furthermore, the morphology and the stability of beads were evaluated.
Results: It was proved that immobilized cells can be preserved the viability and biosurfactant production. Application of full factorial design indicated that the values of three parameters sodium alginate 3%, CaCl2 1% (w/v), and hardening time of 35 min, was found to be too stable for minimum surface tension and stable alginate gel beads.
Conclusions: The alginate gel beads showed stability during the growing process and the immobilized cells efficiently were viable. Alginate source, hardening time, and the interaction between CaCl2 concentration and hardening time influenced on the bead preparation. |
| first_indexed | 2024-12-18T16:48:57Z |
| format | Article |
| id | doaj.art-954884090f804b0493c97063e99a7c80 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-18T16:48:57Z |
| publishDate | 2019-11-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-954884090f804b0493c97063e99a7c802022-12-21T21:00:56ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502019-11-0176413420Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beadsGholamreza Dehghannoudeh0Kioomars Kiani1Mohammad Hassan Moshafi2Negar Dehghannoudeh3Majid Rajaee4Soodeh Salarpour5Mandana Ohadi6Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.Faculty of Arts and Science, University of Toronto, 100 St George St, M5S3G3, Toronto, Ontario, Canada.Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.Context: Biosurfactants are amphipathic molecules that reduce surface tension. The major reasons to economically production of the biosurfactant are their health, safety, environmental management, and promising applications. Aims: To optimize the immobilization of Pseudomonas aeruginosa as a biosurfactant producer in alginate beads. Methods: Biosurfactant production by P. aeruginosa was confirmed through the hemolysis test, emulsification index, and surface activity measurement. Calcium alginate encapsulation technique was used in order to entrap the P. aeruginosa cells. Full factorial design was employed to optimize bead preparation. Furthermore, the morphology and the stability of beads were evaluated. Results: It was proved that immobilized cells can be preserved the viability and biosurfactant production. Application of full factorial design indicated that the values of three parameters sodium alginate 3%, CaCl2 1% (w/v), and hardening time of 35 min, was found to be too stable for minimum surface tension and stable alginate gel beads. Conclusions: The alginate gel beads showed stability during the growing process and the immobilized cells efficiently were viable. Alginate source, hardening time, and the interaction between CaCl2 concentration and hardening time influenced on the bead preparation.http://jppres.com/jppres/pdf/vol7/jppres19.628_7.6.413.pdfalginate beadsbiosurfactantsimmobilizationoptimizingPseudomonas aeruginosa |
| spellingShingle | Gholamreza Dehghannoudeh Kioomars Kiani Mohammad Hassan Moshafi Negar Dehghannoudeh Majid Rajaee Soodeh Salarpour Mandana Ohadi Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads Journal of Pharmacy & Pharmacognosy Research alginate beads biosurfactants immobilization optimizing Pseudomonas aeruginosa |
| title | Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads |
| title_full | Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads |
| title_fullStr | Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads |
| title_full_unstemmed | Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads |
| title_short | Optimizing the immobilization of biosurfactant-producing Pseudomonas aeruginosa in alginate beads |
| title_sort | optimizing the immobilization of biosurfactant producing pseudomonas aeruginosa in alginate beads |
| topic | alginate beads biosurfactants immobilization optimizing Pseudomonas aeruginosa |
| url | http://jppres.com/jppres/pdf/vol7/jppres19.628_7.6.413.pdf |
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