Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia
Bat trypanosomes consist of more than 30 trypanosome species from over 70 species of bats. Recent studies suggest that bats play a role in disseminating trypanosomes from African continent to the terrestrial mammals both in the Afrotropic-Palearctic Ecozones and Nearctic Ecozone. However, the divers...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-08-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224418301792 |
| _version_ | 1818848248188633088 |
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| author | Yongjin Qiu Masahiro Kajihara Hayato Harima Bernard Mudenda Hang'ombe Ryo Nakao Kyoko Hayashida Akina Mori-Kajihara Katendi Changula Yoshiki Eto Joseph Ndebe Reiko Yoshida Yoshihiro Takadate Daniel Mwizabi Hiroki Kawabata Martin Simuunza Aaron Mweene Hirofumi Sawa Ayato Takada Chihiro Sugimoto |
| author_facet | Yongjin Qiu Masahiro Kajihara Hayato Harima Bernard Mudenda Hang'ombe Ryo Nakao Kyoko Hayashida Akina Mori-Kajihara Katendi Changula Yoshiki Eto Joseph Ndebe Reiko Yoshida Yoshihiro Takadate Daniel Mwizabi Hiroki Kawabata Martin Simuunza Aaron Mweene Hirofumi Sawa Ayato Takada Chihiro Sugimoto |
| author_sort | Yongjin Qiu |
| collection | DOAJ |
| description | Bat trypanosomes consist of more than 30 trypanosome species from over 70 species of bats. Recent studies suggest that bats play a role in disseminating trypanosomes from African continent to the terrestrial mammals both in the Afrotropic-Palearctic Ecozones and Nearctic Ecozone. However, the diversity, distribution, and evolution of bat trypanosomes are still unclear. To better understand their evolution, more genetic data of bat trypanosomes from a variety of locations are required. During a survey of Borrelia spp. of bats inhabiting a cave in Zambia, we observed flagellate parasites from 5 of 43 hemocultures. Sequence and phylogenetic analyses of the glycosomal glyceraldehyde 3-phosphate dehydrogenase gene (gGAPDH; 572 bp) and the 18S ribosomal RNA gene (18S rRNA gene; 1,079–1,091 bp) revealed that all were Trypanosoma spp. belonged to the Trypanosoma cruzi clade. Three and two of them exhibited the similarity with T. conorhini and T. dionisii, respectively. The present study provides the first genetic data on Trypanosoma spp. of bats inhabiting Zambia. Keywords: Zambia, Bat, Trypanosoma cruzi clade, Trypanosoma conorhini, Trypanosoma dionisii |
| first_indexed | 2024-12-19T06:14:19Z |
| format | Article |
| id | doaj.art-954a0cc54e3240e1aaf35fcdb8c2bb39 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-12-19T06:14:19Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-954a0cc54e3240e1aaf35fcdb8c2bb392022-12-21T20:32:54ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442019-08-019234238Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in ZambiaYongjin Qiu0Masahiro Kajihara1Hayato Harima2Bernard Mudenda Hang'ombe3Ryo Nakao4Kyoko Hayashida5Akina Mori-Kajihara6Katendi Changula7Yoshiki Eto8Joseph Ndebe9Reiko Yoshida10Yoshihiro Takadate11Daniel Mwizabi12Hiroki Kawabata13Martin Simuunza14Aaron Mweene15Hirofumi Sawa16Ayato Takada17Chihiro Sugimoto18Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, PO Box 32379, Lusaka, 10101, ZambiaDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanHokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, PO Box 32379, Lusaka, 10101, ZambiaDepartment of Para-clinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Virus Network Affilate Center of Excellence, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; African Center of Excellence for Infectious Diseases of Humans and Animals, P.O. Box 32379, Lusaka, 10101, ZambiaLaboratory of Veterinary Parasitology, Graduate School of Infectious Diseases, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, Hokkaido, 060-0818, JapanDivision of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDepartment of Para-clinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka, 10101, ZambiaDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, ZambiaDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDepartment of National Parks and Wildlife, Ministry of Tourism and Arts, Chilanga, 101010, ZambiaDepartment of Bacteriology-I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjyuku-ku, Tokyo, 162-8640, JapanDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, ZambiaGlobal Virus Network Affilate Center of Excellence, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; African Center of Excellence for Infectious Diseases of Humans and Animals, P.O. Box 32379, Lusaka, 10101, Zambia; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, ZambiaGlobal Virus Network Affilate Center of Excellence, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; African Center of Excellence for Infectious Diseases of Humans and Animals, P.O. Box 32379, Lusaka, 10101, Zambia; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Global Virus Network, 725 West Lombard St, Baltimore, MD, 21201, USA; Division of Molecular Pathology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, JapanDivision of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Corresponding author. Division of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-Ku, Sapporo, Hokkaido, 001-0020, Japan.Bat trypanosomes consist of more than 30 trypanosome species from over 70 species of bats. Recent studies suggest that bats play a role in disseminating trypanosomes from African continent to the terrestrial mammals both in the Afrotropic-Palearctic Ecozones and Nearctic Ecozone. However, the diversity, distribution, and evolution of bat trypanosomes are still unclear. To better understand their evolution, more genetic data of bat trypanosomes from a variety of locations are required. During a survey of Borrelia spp. of bats inhabiting a cave in Zambia, we observed flagellate parasites from 5 of 43 hemocultures. Sequence and phylogenetic analyses of the glycosomal glyceraldehyde 3-phosphate dehydrogenase gene (gGAPDH; 572 bp) and the 18S ribosomal RNA gene (18S rRNA gene; 1,079–1,091 bp) revealed that all were Trypanosoma spp. belonged to the Trypanosoma cruzi clade. Three and two of them exhibited the similarity with T. conorhini and T. dionisii, respectively. The present study provides the first genetic data on Trypanosoma spp. of bats inhabiting Zambia. Keywords: Zambia, Bat, Trypanosoma cruzi clade, Trypanosoma conorhini, Trypanosoma dionisiihttp://www.sciencedirect.com/science/article/pii/S2213224418301792 |
| spellingShingle | Yongjin Qiu Masahiro Kajihara Hayato Harima Bernard Mudenda Hang'ombe Ryo Nakao Kyoko Hayashida Akina Mori-Kajihara Katendi Changula Yoshiki Eto Joseph Ndebe Reiko Yoshida Yoshihiro Takadate Daniel Mwizabi Hiroki Kawabata Martin Simuunza Aaron Mweene Hirofumi Sawa Ayato Takada Chihiro Sugimoto Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia International Journal for Parasitology: Parasites and Wildlife |
| title | Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia |
| title_full | Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia |
| title_fullStr | Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia |
| title_full_unstemmed | Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia |
| title_short | Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia |
| title_sort | molecular characterization and phylogenetic analysis of trypanosoma spp detected from striped leaf nosed bats hipposideros vittatus in zambia |
| url | http://www.sciencedirect.com/science/article/pii/S2213224418301792 |
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