Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka
Spinyhead croaker (<i>Collichthys lucidus</i>) is an economically important fish suffering from population decline caused by overfishing and habitat destruction. Researches on the development of primordial germ cell (PGC) and reproduction biology were an emergency for the long-term conse...
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MDPI AG
2022-08-01
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| author | Cong Xu Yu Li Zhengshun Wen Muhammad Jawad Lang Gui Mingyou Li |
| author_facet | Cong Xu Yu Li Zhengshun Wen Muhammad Jawad Lang Gui Mingyou Li |
| author_sort | Cong Xu |
| collection | DOAJ |
| description | Spinyhead croaker (<i>Collichthys lucidus</i>) is an economically important fish suffering from population decline caused by overfishing and habitat destruction. Researches on the development of primordial germ cell (PGC) and reproduction biology were an emergency for the long-term conservation of the involved species. <i>Dead end</i> (<i>dnd</i>) gene plays an indispensable role in PGC specification, maintenance, and development. In the current study, we report the cloning and expression patterns of <i>dnd</i> in <i>C. lucidus</i> (<i>Cldnd</i>). RT-PCR analysis revealed that <i>Cldnd</i> was specifically expressed in both sexual gonads. In the ovary, <i>Cldnd</i> RNA was uniformly distributed in the oocytes and abundant in oogonia, and gradually decreased with oogenesis. A similar expression pattern was also detected in testis. Dual fluorescent in situ hybridization of <i>Cldnd</i> and <i>Clvasa</i> demonstrated that they almost had the same distribution except in oocytes at stage I, in which the <i>vasa</i> RNA aggregated into some particles. Furthermore, <i>Cldnd</i> 3′ UTR was sufficient to guide the Green Fluorescent Protein (GFP) specifically and stably expressed in the PGCs of medaka. These findings offer insight into that <i>Cldnd</i> is an evolutionarily conserved germline-specific gene and even a potential candidate for PGC manipulation in <i>C. lucidus</i>. |
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| language | English |
| last_indexed | 2024-03-09T09:54:32Z |
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| spelling | doaj.art-954a87adbc984dfe96f22ee7bd1d54a02023-12-01T23:54:36ZengMDPI AGLife2075-17292022-08-01128122610.3390/life12081226Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in MedakaCong Xu0Yu Li1Zhengshun Wen2Muhammad Jawad3Lang Gui4Mingyou Li5Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan 316022, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaSpinyhead croaker (<i>Collichthys lucidus</i>) is an economically important fish suffering from population decline caused by overfishing and habitat destruction. Researches on the development of primordial germ cell (PGC) and reproduction biology were an emergency for the long-term conservation of the involved species. <i>Dead end</i> (<i>dnd</i>) gene plays an indispensable role in PGC specification, maintenance, and development. In the current study, we report the cloning and expression patterns of <i>dnd</i> in <i>C. lucidus</i> (<i>Cldnd</i>). RT-PCR analysis revealed that <i>Cldnd</i> was specifically expressed in both sexual gonads. In the ovary, <i>Cldnd</i> RNA was uniformly distributed in the oocytes and abundant in oogonia, and gradually decreased with oogenesis. A similar expression pattern was also detected in testis. Dual fluorescent in situ hybridization of <i>Cldnd</i> and <i>Clvasa</i> demonstrated that they almost had the same distribution except in oocytes at stage I, in which the <i>vasa</i> RNA aggregated into some particles. Furthermore, <i>Cldnd</i> 3′ UTR was sufficient to guide the Green Fluorescent Protein (GFP) specifically and stably expressed in the PGCs of medaka. These findings offer insight into that <i>Cldnd</i> is an evolutionarily conserved germline-specific gene and even a potential candidate for PGC manipulation in <i>C. lucidus</i>.https://www.mdpi.com/2075-1729/12/8/1226<i>dnd</i><i>Collichthys lucidus</i>PGC |
| spellingShingle | Cong Xu Yu Li Zhengshun Wen Muhammad Jawad Lang Gui Mingyou Li Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka Life <i>dnd</i> <i>Collichthys lucidus</i> PGC |
| title | Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka |
| title_full | Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka |
| title_fullStr | Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka |
| title_full_unstemmed | Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka |
| title_short | Spinyhead Croaker Germ Cells Gene <i>dnd</i> Visualizes Primordial Germ Cells in Medaka |
| title_sort | spinyhead croaker germ cells gene i dnd i visualizes primordial germ cells in medaka |
| topic | <i>dnd</i> <i>Collichthys lucidus</i> PGC |
| url | https://www.mdpi.com/2075-1729/12/8/1226 |
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