Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis
Dioscorea zingiberensis accumulates abundant steroidal saponins, such as dioscin, which is the principal bioactive ingredient displaying a wide range of pharmacological activities. Diosgenin is the aglycone of dioscin, and recently, genes encoding cytochrome P450 enzymes in the late steps of diosgen...
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.713036/full |
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| author | Jia Li Isidore Mosongo Han Li Yalun Wu Changfu Li Shihui Yang Yansheng Zhang |
| author_facet | Jia Li Isidore Mosongo Han Li Yalun Wu Changfu Li Shihui Yang Yansheng Zhang |
| author_sort | Jia Li |
| collection | DOAJ |
| description | Dioscorea zingiberensis accumulates abundant steroidal saponins, such as dioscin, which is the principal bioactive ingredient displaying a wide range of pharmacological activities. Diosgenin is the aglycone of dioscin, and recently, genes encoding cytochrome P450 enzymes in the late steps of diosgenin biosynthesis have been isolated. Diosgenin was successfully synthesized in the cholesterol-producing yeasts. From diosgenin to dioscin, one glucose and two rhamnose groups need to be added. Although genes encoding UDP-glucosyltransferases converting diosgenin to trillin were isolated, genes encoding UDP-rhamnosyltransferases involved in dioscin biosynthesis remain unknown. In this study, we isolated the cDNA encoding the trillin rhamnosyltransferase (designated DzGT1) from D. zingiberensis. Heterologous expression of DzGT1 in Escherichia coli cells showed that the gene product exhibits an enzyme activity that glycosylates the trillin to form prosapogenin A of dioscin (PSA). The transcript level of DzGT1 is in accord with PSA accumulation in different organs of D. zingiberensis. Integration of the biochemical, metabolic, and transcriptional data supported the function of DzGT1 in dioscin biosynthesis. The identification and characterization of DzGT1 will help understand the metabolism of steroidal saponins in D. zingiberensis and provide candidate UDP-rhamnosyltransferase for efficient production of PSA, dioscin, and relevant steroidal saponins in microbial hosts. |
| first_indexed | 2024-12-20T15:18:45Z |
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| id | doaj.art-954b9a7f3ffd421484d02a7c31b379ae |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T15:18:45Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-954b9a7f3ffd421484d02a7c31b379ae2022-12-21T19:36:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-08-011210.3389/fpls.2021.713036713036Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensisJia Li0Isidore Mosongo1Han Li2Yalun Wu3Changfu Li4Shihui Yang5Yansheng Zhang6State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, School of Life Sciences, Hubei University, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, School of Life Sciences, Hubei University, Wuhan, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, School of Life Sciences, Hubei University, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, School of Life Sciences, Hubei University, Wuhan, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, ChinaDioscorea zingiberensis accumulates abundant steroidal saponins, such as dioscin, which is the principal bioactive ingredient displaying a wide range of pharmacological activities. Diosgenin is the aglycone of dioscin, and recently, genes encoding cytochrome P450 enzymes in the late steps of diosgenin biosynthesis have been isolated. Diosgenin was successfully synthesized in the cholesterol-producing yeasts. From diosgenin to dioscin, one glucose and two rhamnose groups need to be added. Although genes encoding UDP-glucosyltransferases converting diosgenin to trillin were isolated, genes encoding UDP-rhamnosyltransferases involved in dioscin biosynthesis remain unknown. In this study, we isolated the cDNA encoding the trillin rhamnosyltransferase (designated DzGT1) from D. zingiberensis. Heterologous expression of DzGT1 in Escherichia coli cells showed that the gene product exhibits an enzyme activity that glycosylates the trillin to form prosapogenin A of dioscin (PSA). The transcript level of DzGT1 is in accord with PSA accumulation in different organs of D. zingiberensis. Integration of the biochemical, metabolic, and transcriptional data supported the function of DzGT1 in dioscin biosynthesis. The identification and characterization of DzGT1 will help understand the metabolism of steroidal saponins in D. zingiberensis and provide candidate UDP-rhamnosyltransferase for efficient production of PSA, dioscin, and relevant steroidal saponins in microbial hosts.https://www.frontiersin.org/articles/10.3389/fpls.2021.713036/fullDioscorea zingiberensissteroidal saponindioscin biosynthesisprosapogenin A of dioscinUDP-rhamnosyltransferasetrillin rhamnosyltransferase (DzGT1) |
| spellingShingle | Jia Li Isidore Mosongo Han Li Yalun Wu Changfu Li Shihui Yang Yansheng Zhang Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis Frontiers in Plant Science Dioscorea zingiberensis steroidal saponin dioscin biosynthesis prosapogenin A of dioscin UDP-rhamnosyltransferase trillin rhamnosyltransferase (DzGT1) |
| title | Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis |
| title_full | Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis |
| title_fullStr | Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis |
| title_full_unstemmed | Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis |
| title_short | Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis |
| title_sort | identification and characterization of a trillin rhamnosyltransferase from dioscorea zingiberensis |
| topic | Dioscorea zingiberensis steroidal saponin dioscin biosynthesis prosapogenin A of dioscin UDP-rhamnosyltransferase trillin rhamnosyltransferase (DzGT1) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.713036/full |
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