Scalable CRISPR-Cas9 chemical genetic screens in non-transformed human cells

Scalable CRISPR-Cas9 chemical genetic screens in non-transformed human cells

Summary: Pooled lentiviral CRISPR-Cas9 screens are utilized for assessing the differential sensitivity or resistance of many single-gene knockouts to a compound. Here, we present a scalable approach for high-throughput compound screening by utilizing a small custom library. We describe steps to perf...

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Bibliographic Details
Main Authors: Kevin Lin, Ya-Chu Chang, Ezequiel Marron Fernandez de Velasco, Kevin Wickman, Chad L. Myers, Anja-Katrin Bielinsky
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
CRISPR
Genomics
High Throughput Screening
Molecular Biology
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200555X

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