Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway
In recent years, obesity has become a key factor affecting human health. Moringa oleifera Lam. is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclea...
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.01447/full |
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| author | Jing Xie Jing Xie Yan Wang Yan Wang Wei-Wei Jiang Wei-Wei Jiang Xuan-Fei Luo Xuan-Fei Luo Tian-Yi Dai Tian-Yi Dai Lei Peng Lei Peng Shuang Song Shuang Song Ling-Fei Li Ling-Fei Li Liang Tao Liang Tao Chong-Ying Shi Ruo-Shi Hao Rong Xiao Yang Tian Yang Tian Jun Sheng Jun Sheng Jun Sheng |
| author_facet | Jing Xie Jing Xie Yan Wang Yan Wang Wei-Wei Jiang Wei-Wei Jiang Xuan-Fei Luo Xuan-Fei Luo Tian-Yi Dai Tian-Yi Dai Lei Peng Lei Peng Shuang Song Shuang Song Ling-Fei Li Ling-Fei Li Liang Tao Liang Tao Chong-Ying Shi Ruo-Shi Hao Rong Xiao Yang Tian Yang Tian Jun Sheng Jun Sheng Jun Sheng |
| author_sort | Jing Xie |
| collection | DOAJ |
| description | In recent years, obesity has become a key factor affecting human health. Moringa oleifera Lam. is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclear. In this study, we elucidated the inhibitory effect of M. oleifera leaf petroleum ether extract (MOPEE) on lipid accumulation by in vitro and in vivo experiments, and we described its mechanism of action. MOPEE suppressed adipogenesis in 3T3-L1 adipocytes in a dose-dependent manner and had no effect on cell viability at doses up to 400 μg/ml. Furthermore, MOPEE (400 μg/ml) significantly downregulated the expression of adipogenesis-associated proteins [peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins α and β (C/EBPα and C/EBPβ), and fatty acid synthase (FAS)] and upregulated the expression of a lipolysis-associated protein [hormone-sensitive lipase (HSL)] in 3T3-L1 adipocytes. Additionally, MOPEE (400 μg/ml) significantly increased the degree of phosphorylation of AMP-activated protein kinase α (AMPKα) and acetyl-CoA carboxylase (ACC). An AMPK inhibitor reversed the MOPEE-induced activation of AMPKα and ACC in 3T3-L1 adipocytes. Animal experiments showed that, in high-fat diet (HFD) mice, MOPEE [0.5 g/kg body weight (BW)] effectively decreased BW; relative epididymal, perirenal, and mesenteric fat weight and fat tissue size; and hepatic fat accumulation. Furthermore, MOPEE markedly reduced the serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST). Moreover, MOPEE significantly downregulated the expression of adipogenesis-associated proteins (PPARγ and FAS) and upregulated the expression of a lipolysis-associated protein [adipose triglyceride lipase (ATGL)] in HFD mice hepatic and epididymal fat tissue. Additionally, MOPEE markedly increased the degree of phosphorylation of AMPKα and ACC in HFD mice hepatic and epididymal fat tissue. Following ultrahigh-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) analysis, three phytocompounds (isoquercitrin, chrysin-7-glucoside, and quercitrin) were identified as compounds with relatively high levels in MOPEE. Among them, quercitrin showed excellent fat accumulation inhibitory activity, and the three compounds had synergistic effects in inhibiting adipogenesis. Taken together, MOPEE inhibits fat accumulation by inhibiting the adipogenesis and promoting the lipolysis, and this process is related to AMPK activation. |
| first_indexed | 2024-12-22T16:57:55Z |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-22T16:57:55Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-954d71c23ad14c8c93e71fcb1e9ef1a22022-12-21T18:19:25ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-12-01910.3389/fphar.2018.01447403175Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling PathwayJing Xie0Jing Xie1Yan Wang2Yan Wang3Wei-Wei Jiang4Wei-Wei Jiang5Xuan-Fei Luo6Xuan-Fei Luo7Tian-Yi Dai8Tian-Yi Dai9Lei Peng10Lei Peng11Shuang Song12Shuang Song13Ling-Fei Li14Ling-Fei Li15Liang Tao16Liang Tao17Chong-Ying Shi18Ruo-Shi Hao19Rong Xiao20Yang Tian21Yang Tian22Jun Sheng23Jun Sheng24Jun Sheng25Yunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Science, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaResearch Institute of Plateau Characteristic Agricultural Industry, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaResearch Institute of Plateau Characteristic Agricultural Industry, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Provincial Key Laboratory of Biological Big Data, Yunnan Agricultural University, Kunming, ChinaResearch Institute of Plateau Characteristic Agricultural Industry, Kunming, ChinaKey Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaIn recent years, obesity has become a key factor affecting human health. Moringa oleifera Lam. is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclear. In this study, we elucidated the inhibitory effect of M. oleifera leaf petroleum ether extract (MOPEE) on lipid accumulation by in vitro and in vivo experiments, and we described its mechanism of action. MOPEE suppressed adipogenesis in 3T3-L1 adipocytes in a dose-dependent manner and had no effect on cell viability at doses up to 400 μg/ml. Furthermore, MOPEE (400 μg/ml) significantly downregulated the expression of adipogenesis-associated proteins [peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding proteins α and β (C/EBPα and C/EBPβ), and fatty acid synthase (FAS)] and upregulated the expression of a lipolysis-associated protein [hormone-sensitive lipase (HSL)] in 3T3-L1 adipocytes. Additionally, MOPEE (400 μg/ml) significantly increased the degree of phosphorylation of AMP-activated protein kinase α (AMPKα) and acetyl-CoA carboxylase (ACC). An AMPK inhibitor reversed the MOPEE-induced activation of AMPKα and ACC in 3T3-L1 adipocytes. Animal experiments showed that, in high-fat diet (HFD) mice, MOPEE [0.5 g/kg body weight (BW)] effectively decreased BW; relative epididymal, perirenal, and mesenteric fat weight and fat tissue size; and hepatic fat accumulation. Furthermore, MOPEE markedly reduced the serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST). Moreover, MOPEE significantly downregulated the expression of adipogenesis-associated proteins (PPARγ and FAS) and upregulated the expression of a lipolysis-associated protein [adipose triglyceride lipase (ATGL)] in HFD mice hepatic and epididymal fat tissue. Additionally, MOPEE markedly increased the degree of phosphorylation of AMPKα and ACC in HFD mice hepatic and epididymal fat tissue. Following ultrahigh-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) analysis, three phytocompounds (isoquercitrin, chrysin-7-glucoside, and quercitrin) were identified as compounds with relatively high levels in MOPEE. Among them, quercitrin showed excellent fat accumulation inhibitory activity, and the three compounds had synergistic effects in inhibiting adipogenesis. Taken together, MOPEE inhibits fat accumulation by inhibiting the adipogenesis and promoting the lipolysis, and this process is related to AMPK activation.https://www.frontiersin.org/article/10.3389/fphar.2018.01447/fullMoringa oleifera leaf petroleum ether extract3T3-L1 adipocytesadipogenesislipolysisAMPKantiobesity |
| spellingShingle | Jing Xie Jing Xie Yan Wang Yan Wang Wei-Wei Jiang Wei-Wei Jiang Xuan-Fei Luo Xuan-Fei Luo Tian-Yi Dai Tian-Yi Dai Lei Peng Lei Peng Shuang Song Shuang Song Ling-Fei Li Ling-Fei Li Liang Tao Liang Tao Chong-Ying Shi Ruo-Shi Hao Rong Xiao Yang Tian Yang Tian Jun Sheng Jun Sheng Jun Sheng Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway Frontiers in Pharmacology Moringa oleifera leaf petroleum ether extract 3T3-L1 adipocytes adipogenesis lipolysis AMPK antiobesity |
| title | Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway |
| title_full | Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway |
| title_fullStr | Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway |
| title_full_unstemmed | Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway |
| title_short | Moringa oleifera Leaf Petroleum Ether Extract Inhibits Lipogenesis by Activating the AMPK Signaling Pathway |
| title_sort | moringa oleifera leaf petroleum ether extract inhibits lipogenesis by activating the ampk signaling pathway |
| topic | Moringa oleifera leaf petroleum ether extract 3T3-L1 adipocytes adipogenesis lipolysis AMPK antiobesity |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.01447/full |
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