Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus
Background and Aims: Nowadays, due to the toxic effects of synthetic compounds, we are witnessing a marked increase in the use of natural types, such as plant extracts. The present study aimed to assess the total phenolic and flavonoid content and in vitro comparative study of the biological activit...
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| Format: | Article |
| Language: | fas |
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Birjand University of Medical Sciences and Health Services
2023-10-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
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| Online Access: | http://journal.bums.ac.ir/article-1-3295-en.html |
| _version_ | 1797660054539730944 |
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| author | Mina Baharlouei Mojtaba Ranjbar |
| author_facet | Mina Baharlouei Mojtaba Ranjbar |
| author_sort | Mina Baharlouei |
| collection | DOAJ |
| description | Background and Aims: Nowadays, due to the toxic effects of synthetic compounds, we are witnessing a marked increase in the use of natural types, such as plant extracts. The present study aimed to assess the total phenolic and flavonoid content and in vitro comparative study of the biological activities of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa.
Materials and Methods: This study measured the total phenolic and flavonoid contents. Thereafter, antioxidant capacity was measured by the DPPH free radical scavenging and reducing power methods. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using the broth microdilution method.
Results: Based on the results, total phenol and flavonoid contents varied from 6.1 to 37.7 mg GAE/g dw and 6.2 to 56.2 mg QE /g dw in the studied species. The species showed high antioxidant capacities. The extracts from the studied species exhibited significant antioxidant activity, with the highest percentage of DPPH free radical inhibition being attributed to the methanolic extract of Carum carvi at a concentration of 400 μg/mL (74.67%) and the highest iron-reducing capacity being attributed to the methanolic extract of Carum carvi at a concentration of 1000 μg/mL (54.0%). The results also pointed out that the methanolic extracts of all three plants, Cuminum cyminum, Carum carvi, and Nigella sativa, have antibacterial activity against four bacterial species: Escherichia coli (PTCC1399), clinical Salmonella typhimurium, Bacillus cereus (PTCC1015), and Staphylococcus aureus (ATCC25923). The lowest inhibitory concentration (25.6 mg/ml) and the lowest bactericidal concentration (5.12 mg/ml) were observed for the methanolic extract of Nigella sativa against E. coli, while among the aqueous extracts, only the aqueous extracts of Cuminum cyminum and Nigella sativa exhibited antibacterial effects against the four studied species.
Conclusion: As evidenced by the results of this study, methanolic extract of three Cuminum cyminum, Carum carvi, and Nigella sativa had significant antioxidant and antibacterial activity against pathogenic bacteria. Therefore, these extracts can be considered natural herbal products for the management of bacterial infections.
*Corresponding Author: Mojtaba Ranjbar; Emails: ranjbarf@ausmt.ac.ir
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| first_indexed | 2024-03-11T18:24:13Z |
| format | Article |
| id | doaj.art-954f5735613040acb1267f66db5de4b1 |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-03-11T18:24:13Z |
| publishDate | 2023-10-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-954f5735613040acb1267f66db5de4b12023-10-14T09:44:38ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522023-10-01302164175Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureusMina Baharlouei0Mojtaba Ranjbar1 Amol university of special modern technologies Amol university of special modern technologies Background and Aims: Nowadays, due to the toxic effects of synthetic compounds, we are witnessing a marked increase in the use of natural types, such as plant extracts. The present study aimed to assess the total phenolic and flavonoid content and in vitro comparative study of the biological activities of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa. Materials and Methods: This study measured the total phenolic and flavonoid contents. Thereafter, antioxidant capacity was measured by the DPPH free radical scavenging and reducing power methods. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using the broth microdilution method. Results: Based on the results, total phenol and flavonoid contents varied from 6.1 to 37.7 mg GAE/g dw and 6.2 to 56.2 mg QE /g dw in the studied species. The species showed high antioxidant capacities. The extracts from the studied species exhibited significant antioxidant activity, with the highest percentage of DPPH free radical inhibition being attributed to the methanolic extract of Carum carvi at a concentration of 400 μg/mL (74.67%) and the highest iron-reducing capacity being attributed to the methanolic extract of Carum carvi at a concentration of 1000 μg/mL (54.0%). The results also pointed out that the methanolic extracts of all three plants, Cuminum cyminum, Carum carvi, and Nigella sativa, have antibacterial activity against four bacterial species: Escherichia coli (PTCC1399), clinical Salmonella typhimurium, Bacillus cereus (PTCC1015), and Staphylococcus aureus (ATCC25923). The lowest inhibitory concentration (25.6 mg/ml) and the lowest bactericidal concentration (5.12 mg/ml) were observed for the methanolic extract of Nigella sativa against E. coli, while among the aqueous extracts, only the aqueous extracts of Cuminum cyminum and Nigella sativa exhibited antibacterial effects against the four studied species. Conclusion: As evidenced by the results of this study, methanolic extract of three Cuminum cyminum, Carum carvi, and Nigella sativa had significant antioxidant and antibacterial activity against pathogenic bacteria. Therefore, these extracts can be considered natural herbal products for the management of bacterial infections. *Corresponding Author: Mojtaba Ranjbar; Emails: ranjbarf@ausmt.ac.ir View ORCID iD Profile You can also search for this author in: PubMed ResearchGate Scopus Google Scholar Google Scholar Profilehttp://journal.bums.ac.ir/article-1-3295-en.htmlantioxidant activityantimicrobial activitycuminum cyminumcarum carvinigella sativa |
| spellingShingle | Mina Baharlouei Mojtaba Ranjbar Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus مجله دانشگاه علوم پزشکی بیرجند antioxidant activity antimicrobial activity cuminum cyminum carum carvi nigella sativa |
| title | Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus |
| title_full | Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus |
| title_fullStr | Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus |
| title_full_unstemmed | Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus |
| title_short | Antioxidant and antimicrobial effects of methanolic and aqueous extracts of Cuminum cyminum, Carum carvi, and Nigella sativa against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus |
| title_sort | antioxidant and antimicrobial effects of methanolic and aqueous extracts of cuminum cyminum carum carvi and nigella sativa against escherichia coli salmonella typhimurium bacillus cereus and staphylococcus aureus |
| topic | antioxidant activity antimicrobial activity cuminum cyminum carum carvi nigella sativa |
| url | http://journal.bums.ac.ir/article-1-3295-en.html |
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