Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were col...
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| Format: | Article |
| Language: | English |
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Institute for Animal Husbandry, Belgrade
2018-01-01
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| Series: | Biotechnology in Animal Husbandry |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2018/1450-91561802239K.pdf |
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| author | Krnjaja Vesna Stanković Slavica Lukić Miloš Mićić Nenad Petrović Tanja Bijelić Zorica Mandić Violeta |
| author_facet | Krnjaja Vesna Stanković Slavica Lukić Miloš Mićić Nenad Petrović Tanja Bijelić Zorica Mandić Violeta |
| author_sort | Krnjaja Vesna |
| collection | DOAJ |
| description | This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain. |
| first_indexed | 2024-12-10T18:57:36Z |
| format | Article |
| id | doaj.art-954f610b22c047f1809ff5d2c993386a |
| institution | Directory Open Access Journal |
| issn | 1450-9156 2217-7140 |
| language | English |
| last_indexed | 2024-12-10T18:57:36Z |
| publishDate | 2018-01-01 |
| publisher | Institute for Animal Husbandry, Belgrade |
| record_format | Article |
| series | Biotechnology in Animal Husbandry |
| spelling | doaj.art-954f610b22c047f1809ff5d2c993386a2022-12-22T01:37:07ZengInstitute for Animal Husbandry, BelgradeBiotechnology in Animal Husbandry1450-91562217-71402018-01-013422392491450-91561802239KToxigenic fungal and mycotoxin contamination of maize samples from different districts in SerbiaKrnjaja Vesna0Stanković Slavica1Lukić Miloš2Mićić Nenad3Petrović Tanja4Bijelić Zorica5Mandić Violeta6Institute for Animal Husbandry, Zemun, BelgradeMaize Research Institute 'Zemun Polje', Zemun Polje, BelgradeInstitute for Animal Husbandry, Zemun, BelgradeInstitute for Animal Husbandry, Zemun, BelgradeUniversity of Belgrade, Faculty of Agriculture, Institute of Food Technology and Biochemistry, BelgradeInstitute for Animal Husbandry, Zemun, BelgradeInstitute for Animal Husbandry, Zemun, BelgradeThis study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2018/1450-91561802239K.pdfmaizetoxigenic fungimycotoxins |
| spellingShingle | Krnjaja Vesna Stanković Slavica Lukić Miloš Mićić Nenad Petrović Tanja Bijelić Zorica Mandić Violeta Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia Biotechnology in Animal Husbandry maize toxigenic fungi mycotoxins |
| title | Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia |
| title_full | Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia |
| title_fullStr | Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia |
| title_full_unstemmed | Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia |
| title_short | Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia |
| title_sort | toxigenic fungal and mycotoxin contamination of maize samples from different districts in serbia |
| topic | maize toxigenic fungi mycotoxins |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2018/1450-91561802239K.pdf |
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