Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies
Naturally occurring antagonists towards pathogens play an important role to avoid pathogen outbreaks in ecosystems, and they can be applied as biocontrol agents for crops. Lichens present long-living symbiotic systems continuously exposed to pathogens. To analyze the antagonistic potential in lichen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00620/full |
| _version_ | 1819180622601519104 |
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| author | Tomislav eCernava Henry eMueller Ines Aline Aschenbrenner Ines Aline Aschenbrenner Martin eGrube Gabriele eBerg |
| author_facet | Tomislav eCernava Henry eMueller Ines Aline Aschenbrenner Ines Aline Aschenbrenner Martin eGrube Gabriele eBerg |
| author_sort | Tomislav eCernava |
| collection | DOAJ |
| description | Naturally occurring antagonists towards pathogens play an important role to avoid pathogen outbreaks in ecosystems, and they can be applied as biocontrol agents for crops. Lichens present long-living symbiotic systems continuously exposed to pathogens. To analyze the antagonistic potential in lichens, we studied the bacterial community active against model bacteria and fungi by an integrative approach combining isolate screening, omics techniques and high resolution mass spectrometry. The highly diverse microbiome of the lung lichen (Lobaria pulmonaria (L.) Hoffm.) included an abundant antagonistic community dominated by Stenotrophomonas, Pseudomonas and Burkholderia. While antagonists represent 24.5% of the isolates, they were identified with only 7% in the metagenome; which means that they were overrepresented in the culturable fraction. Isolates of the dominant antagonistic genus Stenotrophomonas produced spermidine as main bioactive component. Moreover, spermidine-related genes, especially for the transport, were identified in the metagenome. The majority of hits identified belonged to Alphaproteobacteria, while Stenotrophomonas-specific spermidine synthases were not present in the dataset. Evidence for plant growth promoting effects was found for lichen-associated strains of Stenotrophomonas. Linking of metagenomic and culture data was possible but showed partly contradictory results, which required a comparative assessment. However, we have shown that lichens are important reservoirs for antagonistic bacteria, which open broad possibilities for biotechnological applications. |
| first_indexed | 2024-12-22T22:17:16Z |
| format | Article |
| id | doaj.art-9552d11d85a34f6b9942c1f06bfdd6a4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T22:17:16Z |
| publishDate | 2015-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9552d11d85a34f6b9942c1f06bfdd6a42022-12-21T18:10:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-06-01610.3389/fmicb.2015.00620146739Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studiesTomislav eCernava0Henry eMueller1Ines Aline Aschenbrenner2Ines Aline Aschenbrenner3Martin eGrube4Gabriele eBerg5Graz University of TechnologyGraz University of TechnologyGraz University of TechnologyUniversity of GrazUniversity of GrazGraz University of TechnologyNaturally occurring antagonists towards pathogens play an important role to avoid pathogen outbreaks in ecosystems, and they can be applied as biocontrol agents for crops. Lichens present long-living symbiotic systems continuously exposed to pathogens. To analyze the antagonistic potential in lichens, we studied the bacterial community active against model bacteria and fungi by an integrative approach combining isolate screening, omics techniques and high resolution mass spectrometry. The highly diverse microbiome of the lung lichen (Lobaria pulmonaria (L.) Hoffm.) included an abundant antagonistic community dominated by Stenotrophomonas, Pseudomonas and Burkholderia. While antagonists represent 24.5% of the isolates, they were identified with only 7% in the metagenome; which means that they were overrepresented in the culturable fraction. Isolates of the dominant antagonistic genus Stenotrophomonas produced spermidine as main bioactive component. Moreover, spermidine-related genes, especially for the transport, were identified in the metagenome. The majority of hits identified belonged to Alphaproteobacteria, while Stenotrophomonas-specific spermidine synthases were not present in the dataset. Evidence for plant growth promoting effects was found for lichen-associated strains of Stenotrophomonas. Linking of metagenomic and culture data was possible but showed partly contradictory results, which required a comparative assessment. However, we have shown that lichens are important reservoirs for antagonistic bacteria, which open broad possibilities for biotechnological applications.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00620/fullSpermidineStenotrophomonasplant growth promotionlichenAntagonistic Bacteria |
| spellingShingle | Tomislav eCernava Henry eMueller Ines Aline Aschenbrenner Ines Aline Aschenbrenner Martin eGrube Gabriele eBerg Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies Frontiers in Microbiology Spermidine Stenotrophomonas plant growth promotion lichen Antagonistic Bacteria |
| title | Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies |
| title_full | Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies |
| title_fullStr | Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies |
| title_full_unstemmed | Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies |
| title_short | Analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies |
| title_sort | analyzing the antagonistic potential of the lichen microbiome against pathogens by bridging metagenomic with culture studies |
| topic | Spermidine Stenotrophomonas plant growth promotion lichen Antagonistic Bacteria |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00620/full |
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