Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction
In this study, the fermentation broth of the recombinant <i>Pichia pastoris</i> strain ncy-2 was studied. After pretreatment, separation, and purification, lysozyme was optimized using biofilm and ion exchange separation. Finally, lysozyme dry enzyme powder was prepared by concentrating...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/8/7/297 |
| _version_ | 1797433608701476864 |
|---|---|
| author | Shanshan Chen Yaqing Tan Yaqing Zhu Liqin Sun Jian Lin Hailing Zhang |
| author_facet | Shanshan Chen Yaqing Tan Yaqing Zhu Liqin Sun Jian Lin Hailing Zhang |
| author_sort | Shanshan Chen |
| collection | DOAJ |
| description | In this study, the fermentation broth of the recombinant <i>Pichia pastoris</i> strain ncy-2 was studied. After pretreatment, separation, and purification, lysozyme was optimized using biofilm and ion exchange separation. Finally, lysozyme dry enzyme powder was prepared by concentrating and vacuum drying. The removal rate of bacterial cells was 99.99% when the fermentation broth was centrifuged at low temperature. The optimum conditions were: transmembrane pressure of 0.20 MPa, pH 6.5, 96.6% yield of lysozyme, enzyme activity of 2612.1 u/mg, which was 1.78 times higher than that of the original enzyme; D152 resin was used for adsorption and elution. Process conditions were optimized: the volume ratio of resin to liquid was 15%; the adsorption time was 4 h; the concentration of NaCl was 1.0 mol/L; the recovery rate of lysozyme activity was 95.67%; the enzyme activity was 3879.6 u/mL; and the purification multiple was 0.5, 3.1 times of the original enzyme activity. The enzyme activity of lysozyme dry enzyme powder was 12,573.6 u/mg, which had an inhibitory effect on microsphere lysozyme. Its enzymatic properties were almost the same as those of natural lysozyme, which demonstrated good application prospects and production potential. |
| first_indexed | 2024-03-09T10:19:25Z |
| format | Article |
| id | doaj.art-955355d4239a4a4688db37c0a5a15617 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T10:19:25Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-955355d4239a4a4688db37c0a5a156172023-12-01T22:08:08ZengMDPI AGFermentation2311-56372022-06-018729710.3390/fermentation8070297Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and ExtractionShanshan Chen0Yaqing Tan1Yaqing Zhu2Liqin Sun3Jian Lin4Hailing Zhang5College of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaIn this study, the fermentation broth of the recombinant <i>Pichia pastoris</i> strain ncy-2 was studied. After pretreatment, separation, and purification, lysozyme was optimized using biofilm and ion exchange separation. Finally, lysozyme dry enzyme powder was prepared by concentrating and vacuum drying. The removal rate of bacterial cells was 99.99% when the fermentation broth was centrifuged at low temperature. The optimum conditions were: transmembrane pressure of 0.20 MPa, pH 6.5, 96.6% yield of lysozyme, enzyme activity of 2612.1 u/mg, which was 1.78 times higher than that of the original enzyme; D152 resin was used for adsorption and elution. Process conditions were optimized: the volume ratio of resin to liquid was 15%; the adsorption time was 4 h; the concentration of NaCl was 1.0 mol/L; the recovery rate of lysozyme activity was 95.67%; the enzyme activity was 3879.6 u/mL; and the purification multiple was 0.5, 3.1 times of the original enzyme activity. The enzyme activity of lysozyme dry enzyme powder was 12,573.6 u/mg, which had an inhibitory effect on microsphere lysozyme. Its enzymatic properties were almost the same as those of natural lysozyme, which demonstrated good application prospects and production potential.https://www.mdpi.com/2311-5637/8/7/297lysozymebiofilmion exchange resinseparationpurification |
| spellingShingle | Shanshan Chen Yaqing Tan Yaqing Zhu Liqin Sun Jian Lin Hailing Zhang Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction Fermentation lysozyme biofilm ion exchange resin separation purification |
| title | Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction |
| title_full | Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction |
| title_fullStr | Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction |
| title_full_unstemmed | Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction |
| title_short | Application of Ultrafiltration and Ion Exchange Separation Technology for Lysozyme Separation and Extraction |
| title_sort | application of ultrafiltration and ion exchange separation technology for lysozyme separation and extraction |
| topic | lysozyme biofilm ion exchange resin separation purification |
| url | https://www.mdpi.com/2311-5637/8/7/297 |
| work_keys_str_mv | AT shanshanchen applicationofultrafiltrationandionexchangeseparationtechnologyforlysozymeseparationandextraction AT yaqingtan applicationofultrafiltrationandionexchangeseparationtechnologyforlysozymeseparationandextraction AT yaqingzhu applicationofultrafiltrationandionexchangeseparationtechnologyforlysozymeseparationandextraction AT liqinsun applicationofultrafiltrationandionexchangeseparationtechnologyforlysozymeseparationandextraction AT jianlin applicationofultrafiltrationandionexchangeseparationtechnologyforlysozymeseparationandextraction AT hailingzhang applicationofultrafiltrationandionexchangeseparationtechnologyforlysozymeseparationandextraction |