Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila
Identifying neural substrates of behavior requires defining actions in terms that map onto brain activity. Brain and muscle activity naturally correlate via the output of motor neurons, but apart from simple movements it has been difficult to define behavior in terms of muscle contractions. By mappi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/68656 |
| _version_ | 1828166226309808128 |
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| author | Amicia D Elliott Adama Berndt Matthew Houpert Snehashis Roy Robert L Scott Carson C Chow Hari Shroff Benjamin H White |
| author_facet | Amicia D Elliott Adama Berndt Matthew Houpert Snehashis Roy Robert L Scott Carson C Chow Hari Shroff Benjamin H White |
| author_sort | Amicia D Elliott |
| collection | DOAJ |
| description | Identifying neural substrates of behavior requires defining actions in terms that map onto brain activity. Brain and muscle activity naturally correlate via the output of motor neurons, but apart from simple movements it has been difficult to define behavior in terms of muscle contractions. By mapping the musculature of the pupal fruit fly and comprehensively imaging muscle activation at single-cell resolution, we here describe a multiphasic behavioral sequence in Drosophila. Our characterization identifies a previously undescribed behavioral phase and permits extraction of major movements by a convolutional neural network. We deconstruct movements into a syllabary of co-active muscles and identify specific syllables that are sensitive to neuromodulatory manipulations. We find that muscle activity shows considerable variability, with sequential increases in stereotypy dependent upon neuromodulation. Our work provides a platform for studying whole-animal behavior, quantifying its variability across multiple spatiotemporal scales, and analyzing its neuromodulatory regulation at cellular resolution. |
| first_indexed | 2024-04-12T01:58:13Z |
| format | Article |
| id | doaj.art-955c4a750d0e43af966bec6ffb598251 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:58:13Z |
| publishDate | 2021-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-955c4a750d0e43af966bec6ffb5982512022-12-22T03:52:45ZengeLife Sciences Publications LtdeLife2050-084X2021-07-011010.7554/eLife.68656Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in DrosophilaAmicia D Elliott0https://orcid.org/0000-0002-3089-1786Adama Berndt1Matthew Houpert2Snehashis Roy3Robert L Scott4Carson C Chow5https://orcid.org/0000-0003-1463-9553Hari Shroff6Benjamin H White7https://orcid.org/0000-0003-0612-8075National Institute of Mental Health, National Institutes of Health, Bethesda, United States; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United StatesNational Institute of Mental Health, National Institutes of Health, Bethesda, United StatesNational Institute of Mental Health, National Institutes of Health, Bethesda, United StatesNational Institute of Mental Health, National Institutes of Health, Bethesda, United StatesNational Institute of Mental Health, National Institutes of Health, Bethesda, United StatesNational Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United StatesNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United StatesNational Institute of Mental Health, National Institutes of Health, Bethesda, United StatesIdentifying neural substrates of behavior requires defining actions in terms that map onto brain activity. Brain and muscle activity naturally correlate via the output of motor neurons, but apart from simple movements it has been difficult to define behavior in terms of muscle contractions. By mapping the musculature of the pupal fruit fly and comprehensively imaging muscle activation at single-cell resolution, we here describe a multiphasic behavioral sequence in Drosophila. Our characterization identifies a previously undescribed behavioral phase and permits extraction of major movements by a convolutional neural network. We deconstruct movements into a syllabary of co-active muscles and identify specific syllables that are sensitive to neuromodulatory manipulations. We find that muscle activity shows considerable variability, with sequential increases in stereotypy dependent upon neuromodulation. Our work provides a platform for studying whole-animal behavior, quantifying its variability across multiple spatiotemporal scales, and analyzing its neuromodulatory regulation at cellular resolution.https://elifesciences.org/articles/68656neural circuitcomputational ethologybursiconCCAPecdysisecdysis triggering hormone |
| spellingShingle | Amicia D Elliott Adama Berndt Matthew Houpert Snehashis Roy Robert L Scott Carson C Chow Hari Shroff Benjamin H White Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila eLife neural circuit computational ethology bursicon CCAP ecdysis ecdysis triggering hormone |
| title | Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila |
| title_full | Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila |
| title_fullStr | Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila |
| title_full_unstemmed | Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila |
| title_short | Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila |
| title_sort | pupal behavior emerges from unstructured muscle activity in response to neuromodulation in drosophila |
| topic | neural circuit computational ethology bursicon CCAP ecdysis ecdysis triggering hormone |
| url | https://elifesciences.org/articles/68656 |
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