A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it>
<p>Abstract</p> <p>Background</p> <p>The best studied insect-symbiont system is that of aphids and their primary bacterial endosymbiont <it>Buchnera aphidicola</it>. <it>Buchnera </it>inhabits specialized host cells called bacteriocytes, provides...
| मुख्य लेखकों: | , , , , , |
|---|---|
| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
BMC
2006-03-01
|
| श्रृंखला: | BMC Genomics |
| ऑनलाइन पहुंच: | http://www.biomedcentral.com/1471-2164/7/50 |
| _version_ | 1828385141043494912 |
|---|---|
| author | Hunter Wayne B Davis Gregory K Dunbar Helen E Wilson Alex CC Stern David L Moran Nancy A |
| author_facet | Hunter Wayne B Davis Gregory K Dunbar Helen E Wilson Alex CC Stern David L Moran Nancy A |
| author_sort | Hunter Wayne B |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The best studied insect-symbiont system is that of aphids and their primary bacterial endosymbiont <it>Buchnera aphidicola</it>. <it>Buchnera </it>inhabits specialized host cells called bacteriocytes, provides nutrients to the aphid and has co-speciated with its aphid hosts for the past 150 million years. We have used a single microarray to examine gene expression in the pea aphid, <it>Acyrthosiphon pisum</it>, and its resident <it>Buchnera</it>. Very little is known of gene expression in aphids, few studies have examined gene expression in <it>Buchnera</it>, and no study has examined simultaneously the expression profiles of a host and its symbiont. Expression profiling of aphids, in studies such as this, will be critical for assigning newly discovered <it>A. pisum </it>genes to functional roles. In particular, because aphids possess many genes that are absent from <it>Drosophila </it>and other holometabolous insect taxa, aphid genome annotation efforts cannot rely entirely on homology to the best-studied insect systems. Development of this dual-genome array represents a first attempt to characterize gene expression in this emerging model system.</p> <p>Results</p> <p>We chose to examine heat shock response because it has been well characterized both in <it>Buchnera </it>and in other insect species. Our results from the <it>Buchnera </it>of <it>A. pisum </it>show responses for the same gene set as an earlier study of heat shock response in <it>Buchnera </it>for the host aphid <it>Schizaphis graminum</it>. Additionally, analyses of aphid transcripts showed the expected response for homologs of known heat shock genes as well as responses for several genes with unknown functional roles.</p> <p>Conclusion</p> <p>We examined gene expression under heat shock of an insect and its bacterial symbiont in a single assay using a dual-genome microarray. Further, our results indicate that microarrays are a useful tool for inferring functional roles of genes in <it>A. pisum </it>and other insects and suggest that the pea aphid genome may contain many gene paralogs that are differentially regulated.</p> |
| first_indexed | 2024-12-10T05:15:19Z |
| format | Article |
| id | doaj.art-955d2ccaae9e4d5ea0a7dea3c5633a19 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-10T05:15:19Z |
| publishDate | 2006-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-955d2ccaae9e4d5ea0a7dea3c5633a192022-12-22T02:00:59ZengBMCBMC Genomics1471-21642006-03-01715010.1186/1471-2164-7-50A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it>Hunter Wayne BDavis Gregory KDunbar Helen EWilson Alex CCStern David LMoran Nancy A<p>Abstract</p> <p>Background</p> <p>The best studied insect-symbiont system is that of aphids and their primary bacterial endosymbiont <it>Buchnera aphidicola</it>. <it>Buchnera </it>inhabits specialized host cells called bacteriocytes, provides nutrients to the aphid and has co-speciated with its aphid hosts for the past 150 million years. We have used a single microarray to examine gene expression in the pea aphid, <it>Acyrthosiphon pisum</it>, and its resident <it>Buchnera</it>. Very little is known of gene expression in aphids, few studies have examined gene expression in <it>Buchnera</it>, and no study has examined simultaneously the expression profiles of a host and its symbiont. Expression profiling of aphids, in studies such as this, will be critical for assigning newly discovered <it>A. pisum </it>genes to functional roles. In particular, because aphids possess many genes that are absent from <it>Drosophila </it>and other holometabolous insect taxa, aphid genome annotation efforts cannot rely entirely on homology to the best-studied insect systems. Development of this dual-genome array represents a first attempt to characterize gene expression in this emerging model system.</p> <p>Results</p> <p>We chose to examine heat shock response because it has been well characterized both in <it>Buchnera </it>and in other insect species. Our results from the <it>Buchnera </it>of <it>A. pisum </it>show responses for the same gene set as an earlier study of heat shock response in <it>Buchnera </it>for the host aphid <it>Schizaphis graminum</it>. Additionally, analyses of aphid transcripts showed the expected response for homologs of known heat shock genes as well as responses for several genes with unknown functional roles.</p> <p>Conclusion</p> <p>We examined gene expression under heat shock of an insect and its bacterial symbiont in a single assay using a dual-genome microarray. Further, our results indicate that microarrays are a useful tool for inferring functional roles of genes in <it>A. pisum </it>and other insects and suggest that the pea aphid genome may contain many gene paralogs that are differentially regulated.</p>http://www.biomedcentral.com/1471-2164/7/50 |
| spellingShingle | Hunter Wayne B Davis Gregory K Dunbar Helen E Wilson Alex CC Stern David L Moran Nancy A A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it> BMC Genomics |
| title | A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it> |
| title_full | A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it> |
| title_fullStr | A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it> |
| title_full_unstemmed | A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it> |
| title_short | A dual-genome microarray for the pea aphid, <it>Acyrthosiphon pisum</it>, and its obligate bacterial symbiont, <it>Buchnera aphidicola</it> |
| title_sort | dual genome microarray for the pea aphid it acyrthosiphon pisum it and its obligate bacterial symbiont it buchnera aphidicola it |
| url | http://www.biomedcentral.com/1471-2164/7/50 |
| work_keys_str_mv | AT hunterwayneb adualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT davisgregoryk adualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT dunbarhelene adualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT wilsonalexcc adualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT sterndavidl adualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT morannancya adualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT hunterwayneb dualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT davisgregoryk dualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT dunbarhelene dualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT wilsonalexcc dualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT sterndavidl dualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait AT morannancya dualgenomemicroarrayforthepeaaphiditacyrthosiphonpisumitanditsobligatebacterialsymbiontitbuchneraaphidicolait |