Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays
Resonant glassy nanostrings have been employed for the detection of biomolecules. These devices offer high sensitivity and amenability to large array integration and multiplexed assays. Such a concept has however been impaired by the lack of stable and biocompatible linker chemistries. Diazonium sal...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-07-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/8/18724 |
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| author | Wei Zheng Rongbing Du Yong Cao Mohammad A. Mohammad Steven K. Dew Mark T. McDermott Stephane Evoy |
| author_facet | Wei Zheng Rongbing Du Yong Cao Mohammad A. Mohammad Steven K. Dew Mark T. McDermott Stephane Evoy |
| author_sort | Wei Zheng |
| collection | DOAJ |
| description | Resonant glassy nanostrings have been employed for the detection of biomolecules. These devices offer high sensitivity and amenability to large array integration and multiplexed assays. Such a concept has however been impaired by the lack of stable and biocompatible linker chemistries. Diazonium salt reduction-induced aryl grafting is an aqueous-based process providing strong chemical adhesion. In this work, diazonium-based linker chemistry was performed for the first time on glassy nanostrings, which enabled the bio-functionalization of such devices. Large arrays of nanostrings with ultra-narrow widths down to 10 nm were fabricated employing electron beam lithography. Diazonium modification was first developed on SiCN surfaces and validated by X-ray photoelectron spectroscopy. Similarly modified nanostrings were then covalently functionalized with anti-rabbit IgG as a molecular probe. Specific enumeration of rabbit IgG was successfully performed through observation of downshifts of resonant frequencies. The specificity of this enumeration was confirmed through proper negative control experiments. Helium ion microscopy further verified the successful functionalization of nanostrings. |
| first_indexed | 2024-04-14T06:41:09Z |
| format | Article |
| id | doaj.art-955d499f7e534fd296936025c73c0f3f |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T06:41:09Z |
| publishDate | 2015-07-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-955d499f7e534fd296936025c73c0f3f2022-12-22T02:07:20ZengMDPI AGSensors1424-82202015-07-01158187241874110.3390/s150818724s150818724Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator ArraysWei Zheng0Rongbing Du1Yong Cao2Mohammad A. Mohammad3Steven K. Dew4Mark T. McDermott5Stephane Evoy6Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, CanadaDepartment of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, AB T6G 2G2, CanadaDepartment of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, AB T6G 2G2, CanadaDepartment of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, CanadaDepartment of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, CanadaDepartment of Chemistry and National Institute for Nanotechnology, University of Alberta, Edmonton, AB T6G 2G2, CanadaDepartment of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, CanadaResonant glassy nanostrings have been employed for the detection of biomolecules. These devices offer high sensitivity and amenability to large array integration and multiplexed assays. Such a concept has however been impaired by the lack of stable and biocompatible linker chemistries. Diazonium salt reduction-induced aryl grafting is an aqueous-based process providing strong chemical adhesion. In this work, diazonium-based linker chemistry was performed for the first time on glassy nanostrings, which enabled the bio-functionalization of such devices. Large arrays of nanostrings with ultra-narrow widths down to 10 nm were fabricated employing electron beam lithography. Diazonium modification was first developed on SiCN surfaces and validated by X-ray photoelectron spectroscopy. Similarly modified nanostrings were then covalently functionalized with anti-rabbit IgG as a molecular probe. Specific enumeration of rabbit IgG was successfully performed through observation of downshifts of resonant frequencies. The specificity of this enumeration was confirmed through proper negative control experiments. Helium ion microscopy further verified the successful functionalization of nanostrings.http://www.mdpi.com/1424-8220/15/8/18724biosensorsnanomechanicsnanostringsinterferometry |
| spellingShingle | Wei Zheng Rongbing Du Yong Cao Mohammad A. Mohammad Steven K. Dew Mark T. McDermott Stephane Evoy Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays Sensors biosensors nanomechanics nanostrings interferometry |
| title | Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays |
| title_full | Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays |
| title_fullStr | Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays |
| title_full_unstemmed | Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays |
| title_short | Diazonium Chemistry for the Bio-Functionalization of Glassy Nanostring Resonator Arrays |
| title_sort | diazonium chemistry for the bio functionalization of glassy nanostring resonator arrays |
| topic | biosensors nanomechanics nanostrings interferometry |
| url | http://www.mdpi.com/1424-8220/15/8/18724 |
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