Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells
Abstract Reduced graphene oxide (rGO) is an graphene oxide (GO) derivative of graphene, which has a large specific surface area and exhibited satisfactory physicochemical characteristics. In this experiment, GO was reduced by PDA to generate PDA-GO complex, and then PDA-GO was combined with Chitosan...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-023-03849-4 |
| _version_ | 1797272974583136256 |
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| author | Yaoyao Li Xinhui Huang Weihao Fu Zonghao Zhang Kuancheng Xiao Hongbing Lv |
| author_facet | Yaoyao Li Xinhui Huang Weihao Fu Zonghao Zhang Kuancheng Xiao Hongbing Lv |
| author_sort | Yaoyao Li |
| collection | DOAJ |
| description | Abstract Reduced graphene oxide (rGO) is an graphene oxide (GO) derivative of graphene, which has a large specific surface area and exhibited satisfactory physicochemical characteristics. In this experiment, GO was reduced by PDA to generate PDA-GO complex, and then PDA-GO was combined with Chitosan (CS) to synthesize PDA-GO/CS composite scaffold. PDA-GO was added to CS to improve the degradation rate of CS, and it was hoped that PDA-GO/CS composite scaffolds could be used in bone tissue engineering. Physicochemical and antimicrobial properties of the different composite scaffolds were examined to find the optimal mass fraction. Besides, we examined the scaffold’s biocompatibility by Phalloidin staining and Live and Dead fluorescent staining. Finally, we applied ALP staining, RT-qPCR, and Alizarin red S staining to detect the effect of PDA-GO/CS on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). The results showed that PDA-GO composite was successfully prepared and PDA-GO/CS composite scaffold was synthesized by combining PDA-GO with CS. Among them, 0.3%PDA-GO/CS scaffolds improves the antibacterial activity and hydrophilicity of CS, while reducing the degradation rate. In vitro, PDA-GO/CS has superior biocompatibility and enhances the early proliferation, migration and osteogenic differentiation of hDPSCs. In conclusion, PDA-GO/CS is a new scaffold materialsuitable for cell culture and has promising application prospect as scaffold for bone tissue engineering. |
| first_indexed | 2024-03-07T14:37:56Z |
| format | Article |
| id | doaj.art-955dd885f93840c38fad9e4d3e38f65e |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-03-07T14:37:56Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-955dd885f93840c38fad9e4d3e38f65e2024-03-05T20:34:08ZengBMCBMC Oral Health1472-68312024-01-0124111710.1186/s12903-023-03849-4Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cellsYaoyao Li0Xinhui Huang1Weihao Fu2Zonghao Zhang3Kuancheng Xiao4Hongbing Lv5Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical UniversityFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical UniversityFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical UniversityFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical UniversityFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical UniversityFujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical UniversityAbstract Reduced graphene oxide (rGO) is an graphene oxide (GO) derivative of graphene, which has a large specific surface area and exhibited satisfactory physicochemical characteristics. In this experiment, GO was reduced by PDA to generate PDA-GO complex, and then PDA-GO was combined with Chitosan (CS) to synthesize PDA-GO/CS composite scaffold. PDA-GO was added to CS to improve the degradation rate of CS, and it was hoped that PDA-GO/CS composite scaffolds could be used in bone tissue engineering. Physicochemical and antimicrobial properties of the different composite scaffolds were examined to find the optimal mass fraction. Besides, we examined the scaffold’s biocompatibility by Phalloidin staining and Live and Dead fluorescent staining. Finally, we applied ALP staining, RT-qPCR, and Alizarin red S staining to detect the effect of PDA-GO/CS on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). The results showed that PDA-GO composite was successfully prepared and PDA-GO/CS composite scaffold was synthesized by combining PDA-GO with CS. Among them, 0.3%PDA-GO/CS scaffolds improves the antibacterial activity and hydrophilicity of CS, while reducing the degradation rate. In vitro, PDA-GO/CS has superior biocompatibility and enhances the early proliferation, migration and osteogenic differentiation of hDPSCs. In conclusion, PDA-GO/CS is a new scaffold materialsuitable for cell culture and has promising application prospect as scaffold for bone tissue engineering.https://doi.org/10.1186/s12903-023-03849-4Maxillofacial bone tissue regenerationHuman dental pulp stem cellsChitosan scaffoldReduced graphene oxidePDA-GO/CS composite scaffoldsTissue engineering |
| spellingShingle | Yaoyao Li Xinhui Huang Weihao Fu Zonghao Zhang Kuancheng Xiao Hongbing Lv Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells BMC Oral Health Maxillofacial bone tissue regeneration Human dental pulp stem cells Chitosan scaffold Reduced graphene oxide PDA-GO/CS composite scaffolds Tissue engineering |
| title | Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells |
| title_full | Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells |
| title_fullStr | Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells |
| title_full_unstemmed | Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells |
| title_short | Preparation of PDA-GO/CS composite scaffold and its effects on the biological properties of human dental pulp stem cells |
| title_sort | preparation of pda go cs composite scaffold and its effects on the biological properties of human dental pulp stem cells |
| topic | Maxillofacial bone tissue regeneration Human dental pulp stem cells Chitosan scaffold Reduced graphene oxide PDA-GO/CS composite scaffolds Tissue engineering |
| url | https://doi.org/10.1186/s12903-023-03849-4 |
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