Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats
20-Hydroxyeicosatetraenoicacid (20-HETE) is an important mediator that regulates vascular tone and blood pressure (BP). Although various experimental animal hypertension models demonstrated that 20-HETE contributes to increased vascular resistance and BP, these effects have not been studied in Nω-ni...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-04-01
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| Series: | Clinical and Experimental Hypertension |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641963.2017.1368540 |
| _version_ | 1797681385091104768 |
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| author | Nur Özen Seher Nasırcılar Ülker Pınar Ülker Filiz Özcan Mutay Aslan Ümit Kemal Şentürk Filiz Basralı |
| author_facet | Nur Özen Seher Nasırcılar Ülker Pınar Ülker Filiz Özcan Mutay Aslan Ümit Kemal Şentürk Filiz Basralı |
| author_sort | Nur Özen |
| collection | DOAJ |
| description | 20-Hydroxyeicosatetraenoicacid (20-HETE) is an important mediator that regulates vascular tone and blood pressure (BP). Although various experimental animal hypertension models demonstrated that 20-HETE contributes to increased vascular resistance and BP, these effects have not been studied in Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced hypertension model. In this study, we investigated the effects of 20-HETE on the vascular responsiveness and BP in an L-NAME-induced hypertension. Wistar Albino rats were used in this study. Hypertension was induced by the addition of L-NAME to drinking water for 5 weeks. The study was performed in three stages: first, BP changes were monitored in real time in the presence of 20-HETE enzymatic inhibitor, N-hydroxy-N´-(4-butly-2-methylphenyl)-formamidine (HET-0016) for 1 h. Second, vascular responses of the conduit and resistance arteries were investigated in the presence or absence of HET-0016 in the organ bath. Third, BP was monitored weekly in some hypertensive animals treated with HET-0016 and vascular responses were investigated at the end of the experiment. We demonstrated an increase in 20-HETE levels in the resistance arteries of hypertensive animals. 20-HETE inhibition by HET-0016 significantly decreased BP in L-NAME-induced hypertension model. In addition, HET-0016 treatment caused significant improvement in vascular dilator and constrictor responses in the conduit and resistance arteries. This study demonstrates an important role of 20-HETE in increasing BP and altering vascular responsiveness in L-NAME-induced hypertension model, which suggests a possible involvement of 20-HETE in essential hypertension development in humans. |
| first_indexed | 2024-03-11T23:44:11Z |
| format | Article |
| id | doaj.art-955f84ceffc84bfe90b60441e7d3d490 |
| institution | Directory Open Access Journal |
| issn | 1064-1963 1525-6006 |
| language | English |
| last_indexed | 2024-03-11T23:44:11Z |
| publishDate | 2018-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Clinical and Experimental Hypertension |
| spelling | doaj.art-955f84ceffc84bfe90b60441e7d3d4902023-09-19T15:19:26ZengTaylor & Francis GroupClinical and Experimental Hypertension1064-19631525-60062018-04-0140329230210.1080/10641963.2017.13685401368540Effect of 20-HETE inhibition on L-NAME-induced hypertension in ratsNur Özen0Seher Nasırcılar Ülker1Pınar Ülker2Filiz Özcan3Mutay Aslan4Ümit Kemal Şentürk5Filiz Basralı6Akdeniz UniversityAkdeniz UniversityAkdeniz UniversityAkdeniz UniversityAkdeniz UniversityAkdeniz UniversityAkdeniz University20-Hydroxyeicosatetraenoicacid (20-HETE) is an important mediator that regulates vascular tone and blood pressure (BP). Although various experimental animal hypertension models demonstrated that 20-HETE contributes to increased vascular resistance and BP, these effects have not been studied in Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced hypertension model. In this study, we investigated the effects of 20-HETE on the vascular responsiveness and BP in an L-NAME-induced hypertension. Wistar Albino rats were used in this study. Hypertension was induced by the addition of L-NAME to drinking water for 5 weeks. The study was performed in three stages: first, BP changes were monitored in real time in the presence of 20-HETE enzymatic inhibitor, N-hydroxy-N´-(4-butly-2-methylphenyl)-formamidine (HET-0016) for 1 h. Second, vascular responses of the conduit and resistance arteries were investigated in the presence or absence of HET-0016 in the organ bath. Third, BP was monitored weekly in some hypertensive animals treated with HET-0016 and vascular responses were investigated at the end of the experiment. We demonstrated an increase in 20-HETE levels in the resistance arteries of hypertensive animals. 20-HETE inhibition by HET-0016 significantly decreased BP in L-NAME-induced hypertension model. In addition, HET-0016 treatment caused significant improvement in vascular dilator and constrictor responses in the conduit and resistance arteries. This study demonstrates an important role of 20-HETE in increasing BP and altering vascular responsiveness in L-NAME-induced hypertension model, which suggests a possible involvement of 20-HETE in essential hypertension development in humans.http://dx.doi.org/10.1080/10641963.2017.136854020-heteblood pressurehet-0016l-namevascular responsiveness |
| spellingShingle | Nur Özen Seher Nasırcılar Ülker Pınar Ülker Filiz Özcan Mutay Aslan Ümit Kemal Şentürk Filiz Basralı Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats Clinical and Experimental Hypertension 20-hete blood pressure het-0016 l-name vascular responsiveness |
| title | Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats |
| title_full | Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats |
| title_fullStr | Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats |
| title_full_unstemmed | Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats |
| title_short | Effect of 20-HETE inhibition on L-NAME-induced hypertension in rats |
| title_sort | effect of 20 hete inhibition on l name induced hypertension in rats |
| topic | 20-hete blood pressure het-0016 l-name vascular responsiveness |
| url | http://dx.doi.org/10.1080/10641963.2017.1368540 |
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