About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line
The association between chemotherapeutic drugs and metal oxide nanoparticles has sparked a rapidly growing interest in cancer nanomedicine. The elaboration of new engineered docetaxel (DTX)-nanocarriers based on titanate nanotubes (TiONts) was reported. The idea was to maintain the drug inside cance...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/10/2733 |
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| author | Alexis Loiseau Julien Boudon Céline Mirjolet Véronique Morgand Nadine Millot |
| author_facet | Alexis Loiseau Julien Boudon Céline Mirjolet Véronique Morgand Nadine Millot |
| author_sort | Alexis Loiseau |
| collection | DOAJ |
| description | The association between chemotherapeutic drugs and metal oxide nanoparticles has sparked a rapidly growing interest in cancer nanomedicine. The elaboration of new engineered docetaxel (DTX)-nanocarriers based on titanate nanotubes (TiONts) was reported. The idea was to maintain the drug inside cancer cells and avoid multidrug resistance mechanisms, which often limit drug efficacy by decreasing their intracellular concentrations in tumor cells. HS-PEG<sub>n</sub>-COOH (PEG: polyethylene glycol, n = 3000, 5000, 10,000) was conjugated, in an organic medium by covalent linkages, on TiONts surface. This study aimed to investigate the influence of different PEG derivatives chain lengths on the TiONts colloidal stability, on the PEG<sub>n</sub> density and conformation, as well as on the DTX biological activity in a prostate cancer model (human PC-3 prostate adenocarcinoma cells). In vitro tests highlighted significant cytotoxicities of the drug after loading DTX on PEG<sub>n</sub>-modified TiONts (TiONts-PEG<sub>n</sub>-DTX). Higher grafting densities for shorter PEGylated chains were most favorable on DTX cytotoxicity by promoting both colloidal stability in biological media and cells internalization. This promising strategy involves a better understanding of nanohybrid engineering, particularly on the PEGylated chain length influence, and can thus become a potent tool in nanomedicine to fight against cancer. |
| first_indexed | 2024-03-10T06:19:22Z |
| format | Article |
| id | doaj.art-9565dcd809224a2c9291c858a71f6f63 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T06:19:22Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-9565dcd809224a2c9291c858a71f6f632023-11-22T19:25:48ZengMDPI AGNanomaterials2079-49912021-10-011110273310.3390/nano11102733About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell LineAlexis Loiseau0Julien Boudon1Céline Mirjolet2Véronique Morgand3Nadine Millot4Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS Université Bourgogne Franche-Comté, BP 47870, CEDEX, 21078 Dijon, FranceLaboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS Université Bourgogne Franche-Comté, BP 47870, CEDEX, 21078 Dijon, FranceINSERM 1231, Cadir Team, CEDEX, 21078 Dijon, FranceRadiotherapy Department, Georges-Francois Leclerc Cancer Center, CEDEX, 21079 Dijon, FranceLaboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS Université Bourgogne Franche-Comté, BP 47870, CEDEX, 21078 Dijon, FranceThe association between chemotherapeutic drugs and metal oxide nanoparticles has sparked a rapidly growing interest in cancer nanomedicine. The elaboration of new engineered docetaxel (DTX)-nanocarriers based on titanate nanotubes (TiONts) was reported. The idea was to maintain the drug inside cancer cells and avoid multidrug resistance mechanisms, which often limit drug efficacy by decreasing their intracellular concentrations in tumor cells. HS-PEG<sub>n</sub>-COOH (PEG: polyethylene glycol, n = 3000, 5000, 10,000) was conjugated, in an organic medium by covalent linkages, on TiONts surface. This study aimed to investigate the influence of different PEG derivatives chain lengths on the TiONts colloidal stability, on the PEG<sub>n</sub> density and conformation, as well as on the DTX biological activity in a prostate cancer model (human PC-3 prostate adenocarcinoma cells). In vitro tests highlighted significant cytotoxicities of the drug after loading DTX on PEG<sub>n</sub>-modified TiONts (TiONts-PEG<sub>n</sub>-DTX). Higher grafting densities for shorter PEGylated chains were most favorable on DTX cytotoxicity by promoting both colloidal stability in biological media and cells internalization. This promising strategy involves a better understanding of nanohybrid engineering, particularly on the PEGylated chain length influence, and can thus become a potent tool in nanomedicine to fight against cancer.https://www.mdpi.com/2079-4991/11/10/2733titanate nanotubespolyethylene glycolcolloidal stabilitydocetaxelcytotoxicityprostate cancer cells |
| spellingShingle | Alexis Loiseau Julien Boudon Céline Mirjolet Véronique Morgand Nadine Millot About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line Nanomaterials titanate nanotubes polyethylene glycol colloidal stability docetaxel cytotoxicity prostate cancer cells |
| title | About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line |
| title_full | About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line |
| title_fullStr | About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line |
| title_full_unstemmed | About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line |
| title_short | About the Influence of PEG Spacers on the Cytotoxicity of Titanate Nanotubes-Docetaxel Nanohybrids against a Prostate Cancer Cell Line |
| title_sort | about the influence of peg spacers on the cytotoxicity of titanate nanotubes docetaxel nanohybrids against a prostate cancer cell line |
| topic | titanate nanotubes polyethylene glycol colloidal stability docetaxel cytotoxicity prostate cancer cells |
| url | https://www.mdpi.com/2079-4991/11/10/2733 |
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