A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome
Increased chicken-derived fat and fructose consumption in the human diet is paralleled by an increasing prevalence of obesity and metabolic syndrome (MS). Herein, we aimed at developing and characterizing a mouse model of diet-induced obesity (DIO) resembling most of the key features of the human MS...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-01-01
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| Series: | Nutrition and Metabolic Insights |
| Online Access: | https://doi.org/10.4137/NMI.S32907 |
| _version_ | 1818926885722128384 |
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| author | Maria C. Della Vedova Marcos D. Muñoz Lucas D. Santillan Maria G. Plateo-Pignatari Maria J. Germanó Martín E. Rinaldi Tosi Silvina Garcia Nidia N. Gomez Miguel W. Fornes Sandra E. Gomez Mejiba Dario C. Ramirez |
| author_facet | Maria C. Della Vedova Marcos D. Muñoz Lucas D. Santillan Maria G. Plateo-Pignatari Maria J. Germanó Martín E. Rinaldi Tosi Silvina Garcia Nidia N. Gomez Miguel W. Fornes Sandra E. Gomez Mejiba Dario C. Ramirez |
| author_sort | Maria C. Della Vedova |
| collection | DOAJ |
| description | Increased chicken-derived fat and fructose consumption in the human diet is paralleled by an increasing prevalence of obesity and metabolic syndrome (MS). Herein, we aimed at developing and characterizing a mouse model of diet-induced obesity (DIO) resembling most of the key features of the human MS. To accomplish this, we fed male C57BL/6J mice for 4, 8, 12, and 16 weeks with either a low-fat diet (LFD) or a high-chicken-fat diet (HFD) and tap water with or without 10% fructose (F). This experimental design resulted in the following four experimental groups: LFD, LFD + F, HFD, and HFD + F. Over the feeding period, and on a weekly basis, the HFD + F group had more caloric intake and gained more weight than the other experimental groups. Compared to the other groups, and at the end of the feeding period, the HFD + F group had a higher adipogenic index, total cholesterol, low-density lipoprotein cholesterol, fasting basal glycemia, insulin resistance, hypertension, and atherogenic index and showed steatohepatitis and systemic oxidative stress/inflammation. A mouse model of DIO that will allow us to study the effect of MS in different organs and systems has been developed and characterized. |
| first_indexed | 2024-12-20T03:04:14Z |
| format | Article |
| id | doaj.art-95677339ab294b1d8a542f22c6dc5056 |
| institution | Directory Open Access Journal |
| issn | 1178-6388 |
| language | English |
| last_indexed | 2024-12-20T03:04:14Z |
| publishDate | 2016-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Nutrition and Metabolic Insights |
| spelling | doaj.art-95677339ab294b1d8a542f22c6dc50562022-12-21T19:55:40ZengSAGE PublishingNutrition and Metabolic Insights1178-63882016-01-01910.4137/NMI.S32907A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic SyndromeMaria C. Della Vedova0Marcos D. Muñoz1Lucas D. Santillan2Maria G. Plateo-Pignatari3Maria J. Germanó4Martín E. Rinaldi Tosi5Silvina Garcia6Nidia N. Gomez7Miguel W. Fornes8Sandra E. Gomez Mejiba9Dario C. Ramirez10Laboratory of Experimental Therapeutics, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Institute for Biochemical Research (INBIO), San Luis, Argentina.Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Laboratory of Morphophysiology, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.LIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-Mendoza-National University of Cuyo, Mendoza, Argentina.Laboratory of Experimental Therapeutics, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.Increased chicken-derived fat and fructose consumption in the human diet is paralleled by an increasing prevalence of obesity and metabolic syndrome (MS). Herein, we aimed at developing and characterizing a mouse model of diet-induced obesity (DIO) resembling most of the key features of the human MS. To accomplish this, we fed male C57BL/6J mice for 4, 8, 12, and 16 weeks with either a low-fat diet (LFD) or a high-chicken-fat diet (HFD) and tap water with or without 10% fructose (F). This experimental design resulted in the following four experimental groups: LFD, LFD + F, HFD, and HFD + F. Over the feeding period, and on a weekly basis, the HFD + F group had more caloric intake and gained more weight than the other experimental groups. Compared to the other groups, and at the end of the feeding period, the HFD + F group had a higher adipogenic index, total cholesterol, low-density lipoprotein cholesterol, fasting basal glycemia, insulin resistance, hypertension, and atherogenic index and showed steatohepatitis and systemic oxidative stress/inflammation. A mouse model of DIO that will allow us to study the effect of MS in different organs and systems has been developed and characterized.https://doi.org/10.4137/NMI.S32907 |
| spellingShingle | Maria C. Della Vedova Marcos D. Muñoz Lucas D. Santillan Maria G. Plateo-Pignatari Maria J. Germanó Martín E. Rinaldi Tosi Silvina Garcia Nidia N. Gomez Miguel W. Fornes Sandra E. Gomez Mejiba Dario C. Ramirez A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome Nutrition and Metabolic Insights |
| title | A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome |
| title_full | A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome |
| title_fullStr | A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome |
| title_full_unstemmed | A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome |
| title_short | A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome |
| title_sort | mouse model of diet induced obesity resembling most features of human metabolic syndrome |
| url | https://doi.org/10.4137/NMI.S32907 |
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