Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl
<i>Salvia lavandulifolia</i> Vahl essential oil is becoming more popular as a cognitive enhancer and treatment for memory loss. It is high in natural antioxidants and has spasmolytic, antiseptic, analgesic, sedative, and anti-inflammatory properties. Its aqueous extract has hypoglycemic...
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MDPI AG
2023-03-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/3/395 |
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| author | Firdaous Remok Soukaina Saidi Aman Allah Gourich Khalid Zibouh Mohamed Maouloua Fadwa El Makhoukhi Naoual El Menyiy Hanane Touijer Mohamed Bouhrim Sevser Sahpaz Ahmad Mohammad Salamatullah Mohammed Bourhia Touriya Zair |
| author_facet | Firdaous Remok Soukaina Saidi Aman Allah Gourich Khalid Zibouh Mohamed Maouloua Fadwa El Makhoukhi Naoual El Menyiy Hanane Touijer Mohamed Bouhrim Sevser Sahpaz Ahmad Mohammad Salamatullah Mohammed Bourhia Touriya Zair |
| author_sort | Firdaous Remok |
| collection | DOAJ |
| description | <i>Salvia lavandulifolia</i> Vahl essential oil is becoming more popular as a cognitive enhancer and treatment for memory loss. It is high in natural antioxidants and has spasmolytic, antiseptic, analgesic, sedative, and anti-inflammatory properties. Its aqueous extract has hypoglycemic activity and is used to treat diabetic hyperglycemia, but few studies have focused on it. The objective of this work is to evaluate the various biological and pharmacological powers of <i>Salvia lavandulifolia</i> Vahl leaf aqueous extract. Quality control of the plant material was first carried out. Followed by a phytochemical study on the aqueous extract of <i>S. lavandulifolia</i> leaves, namely phytochemical screening and determination of total polyphenols, flavonoids, and condensed tannins contents. Then, the biological activities were undertaken, in particular the antioxidant activity (total antioxidant activity and trapping of the DPPH° radical) and the antimicrobial activity. The chemical composition of this extract was also determined by HPLC-MS-ESI. Finally, the inhibitory effect of the α-amylase enzyme as well as the antihyperglycaemic effect was evaluated in vivo in normal rats overloaded with starch or D-glucose. The aqueous extract obtained by use of the decoction of leaves of <i>S. lavandulifolia</i> contains 246.51 ± 1.69 mg EQ of gallic acid/g DE, 23.80 ± 0.12 mg EQ quercetin/g DE, and 2.46 ± 0.08 mg EQ catechin /g DE. Its total antioxidant capacity is around 527.03 ± 5.95 mg EQ of ascorbic acid/g DE. At a concentration of 5.81 ± 0.23 µg/mL, our extract was able to inhibit 50% of DPPH° radicals. Moreover, it showed bactericidal effect against <i>Proteus mirabilis</i>, fungicidal against <i>Aspergillus niger</i>, <i>Candida albicans</i>, <i>Candida tropicalis</i>, and <i>Saccharomyces cerevisiae</i>, and fungistatic against <i>Candida krusei</i>. A marked antihyperglycemic activity (AUC = 54.84 ± 4.88 g/L/h), as well as a significant inhibitory effect of α-amylase in vitro (IC<sub>50</sub> = 0.99 ± 0.00 mg/mL) and in vivo (AUC = 51.94 ± 1.29 g/L/h), is recorded in our extract. Furthermore, its chemical composition reveals the presence of 37.03% rosmarinic acid, 7.84% quercetin rhamnose, 5.57% diosmetin-rutinoside, 5.51% catechin dimer, and 4.57% gallocatechin as major compounds. The antihyperglycemic and α-amylase inhibitory activities, associated with the antioxidant properties of <i>S. lavandulifolia</i>, justify its use in the treatment of diabetes in traditional medicine and highlight its potential introduction into antidiabetic drugs. |
| first_indexed | 2024-03-11T06:03:24Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T06:03:24Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-956861ec879f4d81b13563fe8985cea62023-11-17T13:12:12ZengMDPI AGPharmaceuticals1424-82472023-03-0116339510.3390/ph16030395Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> VahlFirdaous Remok0Soukaina Saidi1Aman Allah Gourich2Khalid Zibouh3Mohamed Maouloua4Fadwa El Makhoukhi5Naoual El Menyiy6Hanane Touijer7Mohamed Bouhrim8Sevser Sahpaz9Ahmad Mohammad Salamatullah10Mohammed Bourhia11Touriya Zair12Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoLaboratory of Medical Biology, Med V Provincial Medical Center, Meknes 50000, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoLaboratory of Pharmacology, National Agency of Medicinal and Aromatic Plants, Taounate 34025, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, MoroccoJoint Research Unit 1158 BioEcoAgro, INRAE, JUNIA, University of Lille, University of Liège, UPJV, University of Artois, ULCO, ICV—Institut Charles Viollette, 59000 Lille, FranceDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaLaboratory of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune 70000, MoroccoResearch Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, Meknes 50070, Morocco<i>Salvia lavandulifolia</i> Vahl essential oil is becoming more popular as a cognitive enhancer and treatment for memory loss. It is high in natural antioxidants and has spasmolytic, antiseptic, analgesic, sedative, and anti-inflammatory properties. Its aqueous extract has hypoglycemic activity and is used to treat diabetic hyperglycemia, but few studies have focused on it. The objective of this work is to evaluate the various biological and pharmacological powers of <i>Salvia lavandulifolia</i> Vahl leaf aqueous extract. Quality control of the plant material was first carried out. Followed by a phytochemical study on the aqueous extract of <i>S. lavandulifolia</i> leaves, namely phytochemical screening and determination of total polyphenols, flavonoids, and condensed tannins contents. Then, the biological activities were undertaken, in particular the antioxidant activity (total antioxidant activity and trapping of the DPPH° radical) and the antimicrobial activity. The chemical composition of this extract was also determined by HPLC-MS-ESI. Finally, the inhibitory effect of the α-amylase enzyme as well as the antihyperglycaemic effect was evaluated in vivo in normal rats overloaded with starch or D-glucose. The aqueous extract obtained by use of the decoction of leaves of <i>S. lavandulifolia</i> contains 246.51 ± 1.69 mg EQ of gallic acid/g DE, 23.80 ± 0.12 mg EQ quercetin/g DE, and 2.46 ± 0.08 mg EQ catechin /g DE. Its total antioxidant capacity is around 527.03 ± 5.95 mg EQ of ascorbic acid/g DE. At a concentration of 5.81 ± 0.23 µg/mL, our extract was able to inhibit 50% of DPPH° radicals. Moreover, it showed bactericidal effect against <i>Proteus mirabilis</i>, fungicidal against <i>Aspergillus niger</i>, <i>Candida albicans</i>, <i>Candida tropicalis</i>, and <i>Saccharomyces cerevisiae</i>, and fungistatic against <i>Candida krusei</i>. A marked antihyperglycemic activity (AUC = 54.84 ± 4.88 g/L/h), as well as a significant inhibitory effect of α-amylase in vitro (IC<sub>50</sub> = 0.99 ± 0.00 mg/mL) and in vivo (AUC = 51.94 ± 1.29 g/L/h), is recorded in our extract. Furthermore, its chemical composition reveals the presence of 37.03% rosmarinic acid, 7.84% quercetin rhamnose, 5.57% diosmetin-rutinoside, 5.51% catechin dimer, and 4.57% gallocatechin as major compounds. The antihyperglycemic and α-amylase inhibitory activities, associated with the antioxidant properties of <i>S. lavandulifolia</i>, justify its use in the treatment of diabetes in traditional medicine and highlight its potential introduction into antidiabetic drugs.https://www.mdpi.com/1424-8247/16/3/395<i>Salvia lavandilufolia</i> Vahldiabetesα-amylaseantioxidantantimicrobial activityquality control |
| spellingShingle | Firdaous Remok Soukaina Saidi Aman Allah Gourich Khalid Zibouh Mohamed Maouloua Fadwa El Makhoukhi Naoual El Menyiy Hanane Touijer Mohamed Bouhrim Sevser Sahpaz Ahmad Mohammad Salamatullah Mohammed Bourhia Touriya Zair Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl Pharmaceuticals <i>Salvia lavandilufolia</i> Vahl diabetes α-amylase antioxidant antimicrobial activity quality control |
| title | Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl |
| title_full | Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl |
| title_fullStr | Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl |
| title_full_unstemmed | Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl |
| title_short | Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of <i>Salvia lavandulifolia</i> Vahl |
| title_sort | phenolic content antioxidant antibacterial antihyperglycemic and α amylase inhibitory activities of aqueous extract of i salvia lavandulifolia i vahl |
| topic | <i>Salvia lavandilufolia</i> Vahl diabetes α-amylase antioxidant antimicrobial activity quality control |
| url | https://www.mdpi.com/1424-8247/16/3/395 |
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