<it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells
<p>Abstract</p> <p>Background</p> <p>Respiratory failure secondary to alveolar inflammation during <it>Pneumocystis </it>pneumonia is a major cause of death in immunocompromised patients. Neutrophil infiltration in the lung of patients with <it>Pneumoc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/11/1/95 |
| _version_ | 1811329214150868992 |
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| author | Limper Andrew H Wylam Mark Xue Ailing Lamont Jeffrey D Carmona Eva M |
| author_facet | Limper Andrew H Wylam Mark Xue Ailing Lamont Jeffrey D Carmona Eva M |
| author_sort | Limper Andrew H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Respiratory failure secondary to alveolar inflammation during <it>Pneumocystis </it>pneumonia is a major cause of death in immunocompromised patients. Neutrophil infiltration in the lung of patients with <it>Pneumocystis </it>infection predicts severity of the infection and death. Several previous studies indicate that airway epithelial cells release the neutrophil chemoattractant proteins, MIP-2 (rodents) and IL-8 (humans), in response to <it>Pneumocystis </it>and purified <it>Pneumocystis </it>cell wall β-glucans (PCBG) through the NF-κB-dependent pathway. However, little is known about the molecular mechanisms that are involved in the activation of airway epithelium cells by PCBG resulting in the secretion of IL-8.</p> <p>Method</p> <p>To address this, we have studied the activation of different calcium-dependent mitogen-activated protein kinases (MAPKs) in 1HAEo<sup>- </sup>cells, a human airway epithelial cell line.</p> <p>Results</p> <p>Our data provide evidence that PCBG induces phosphorylation of the MAPKs, ERK, and p38, the activation of NF-κB and the subsequently secretion of IL-8 in a calcium-dependent manner. Further, we evaluated the role of glycosphingolipids as possible receptors for β-glucans in human airway epithelial cells. Preincubation of the cells with D-<it>threo</it>-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) a potent inhibitor of the glycosphingolipids synthesis, prior to PCBG stimulation, significantly decreased IL-8 production.</p> <p>Conclusion</p> <p>These data indicate that PCBG activates calcium dependent MAPK signaling resulting in the release of IL-8 in a process that requires glycosphingolipid for optimal signaling.</p> |
| first_indexed | 2024-04-13T15:39:50Z |
| format | Article |
| id | doaj.art-956894d2a89b493eb851d7b349a56679 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-13T15:39:50Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-956894d2a89b493eb851d7b349a566792022-12-22T02:41:10ZengBMCRespiratory Research1465-99212010-07-011119510.1186/1465-9921-11-95<it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cellsLimper Andrew HWylam MarkXue AilingLamont Jeffrey DCarmona Eva M<p>Abstract</p> <p>Background</p> <p>Respiratory failure secondary to alveolar inflammation during <it>Pneumocystis </it>pneumonia is a major cause of death in immunocompromised patients. Neutrophil infiltration in the lung of patients with <it>Pneumocystis </it>infection predicts severity of the infection and death. Several previous studies indicate that airway epithelial cells release the neutrophil chemoattractant proteins, MIP-2 (rodents) and IL-8 (humans), in response to <it>Pneumocystis </it>and purified <it>Pneumocystis </it>cell wall β-glucans (PCBG) through the NF-κB-dependent pathway. However, little is known about the molecular mechanisms that are involved in the activation of airway epithelium cells by PCBG resulting in the secretion of IL-8.</p> <p>Method</p> <p>To address this, we have studied the activation of different calcium-dependent mitogen-activated protein kinases (MAPKs) in 1HAEo<sup>- </sup>cells, a human airway epithelial cell line.</p> <p>Results</p> <p>Our data provide evidence that PCBG induces phosphorylation of the MAPKs, ERK, and p38, the activation of NF-κB and the subsequently secretion of IL-8 in a calcium-dependent manner. Further, we evaluated the role of glycosphingolipids as possible receptors for β-glucans in human airway epithelial cells. Preincubation of the cells with D-<it>threo</it>-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) a potent inhibitor of the glycosphingolipids synthesis, prior to PCBG stimulation, significantly decreased IL-8 production.</p> <p>Conclusion</p> <p>These data indicate that PCBG activates calcium dependent MAPK signaling resulting in the release of IL-8 in a process that requires glycosphingolipid for optimal signaling.</p>http://respiratory-research.com/content/11/1/95 |
| spellingShingle | Limper Andrew H Wylam Mark Xue Ailing Lamont Jeffrey D Carmona Eva M <it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells Respiratory Research |
| title | <it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells |
| title_full | <it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells |
| title_fullStr | <it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells |
| title_full_unstemmed | <it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells |
| title_short | <it>Pneumocystis </it>cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells |
| title_sort | it pneumocystis it cell wall β glucan stimulates calcium dependent signaling of il 8 secretion by human airway epithelial cells |
| url | http://respiratory-research.com/content/11/1/95 |
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