Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats
Objective: This study was conceived to investigate the pathogenicity of relapsed (Diminazene aceturate-resistant), field (original) and mixed (relapsed and field) isolates of Trypanosoma brucei brucei in rats. Materials and methods: Twenty eight healthy adult albino rats of both sexes weighing betwe...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Network for the Veterinarians of Bangladesh
2017-03-01
|
| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=229316 |
| _version_ | 1819263636247412736 |
|---|---|
| author | Tobias Nnia Egbe-Nwiyi Ephraim Igwenagu Anastasia Theresa Nwaosu Meshach Maunta Maina |
| author_facet | Tobias Nnia Egbe-Nwiyi Ephraim Igwenagu Anastasia Theresa Nwaosu Meshach Maunta Maina |
| author_sort | Tobias Nnia Egbe-Nwiyi |
| collection | DOAJ |
| description | Objective: This study was conceived to investigate the pathogenicity of relapsed (Diminazene aceturate-resistant), field (original) and mixed (relapsed and field) isolates of Trypanosoma brucei brucei in rats.
Materials and methods: Twenty eight healthy adult albino rats of both sexes weighing between 149-177 gm were used to compare the pathogenicity of relapsed, field and the mixed isolates of T. brucei brucei infections. The rats were separated into four groups (A-D); where, group A was kept as uninfected control, and group B was infected with 1x103 trypanosomes of the field isolate and 1x103 trypanosomes of the diminazene aceturate resistant isolate. The rats of groups C and D were infected with 1x106 trypanosomes of the diminazene aceturate-resistant isolate and 1x106 trypanosomes of the field isolate, respectively.
Results: The infected rats became parasitemic within 4 to 8 days post-infection. The mean pre-patent periods (PP) were 4.1±1.1, 6.0±2.0 and 9.1±1.1 days in groups B, C and D respectively, while the mean survival time (ST) in groups B, C and D were 21.4±10.1, 27.1±13.2 and 34.0 ±12.8 days, respectively. The PP and ST were shortest (P<0.05) in group B (mixed infections), and level of parasitemia was higher (P<0.05) in group B (mixed infections) as compared to groups C and D. The level of anemia was comparable (P>0.05) in groups C and D and more severe (P<0.05) in group B.
Conclusion: Mixed infections exhibit shortest PP, ST, higher level of parasitemia and more severe anemia, and appear to be more pathogenic. [J Adv Vet Anim Res 2017; 4(1.000): 97-103] |
| first_indexed | 2024-12-23T20:16:44Z |
| format | Article |
| id | doaj.art-9568e82aa0e54452a627c05dcd6cef9f |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-23T20:16:44Z |
| publishDate | 2017-03-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-9568e82aa0e54452a627c05dcd6cef9f2022-12-21T17:32:40ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102017-03-01419710310.5455/javar.2017.d197229316Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in ratsTobias Nnia Egbe-Nwiyi0Ephraim Igwenagu1Anastasia Theresa Nwaosu2Meshach Maunta Maina3Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Maiduguri, NigeriaObjective: This study was conceived to investigate the pathogenicity of relapsed (Diminazene aceturate-resistant), field (original) and mixed (relapsed and field) isolates of Trypanosoma brucei brucei in rats. Materials and methods: Twenty eight healthy adult albino rats of both sexes weighing between 149-177 gm were used to compare the pathogenicity of relapsed, field and the mixed isolates of T. brucei brucei infections. The rats were separated into four groups (A-D); where, group A was kept as uninfected control, and group B was infected with 1x103 trypanosomes of the field isolate and 1x103 trypanosomes of the diminazene aceturate resistant isolate. The rats of groups C and D were infected with 1x106 trypanosomes of the diminazene aceturate-resistant isolate and 1x106 trypanosomes of the field isolate, respectively. Results: The infected rats became parasitemic within 4 to 8 days post-infection. The mean pre-patent periods (PP) were 4.1±1.1, 6.0±2.0 and 9.1±1.1 days in groups B, C and D respectively, while the mean survival time (ST) in groups B, C and D were 21.4±10.1, 27.1±13.2 and 34.0 ±12.8 days, respectively. The PP and ST were shortest (P<0.05) in group B (mixed infections), and level of parasitemia was higher (P<0.05) in group B (mixed infections) as compared to groups C and D. The level of anemia was comparable (P>0.05) in groups C and D and more severe (P<0.05) in group B. Conclusion: Mixed infections exhibit shortest PP, ST, higher level of parasitemia and more severe anemia, and appear to be more pathogenic. [J Adv Vet Anim Res 2017; 4(1.000): 97-103]http://www.ejmanager.com/fulltextpdf.php?mno=229316Field isolate; Mixed infections; Pathogenicity; Parasitemia; Relapsed isolate |
| spellingShingle | Tobias Nnia Egbe-Nwiyi Ephraim Igwenagu Anastasia Theresa Nwaosu Meshach Maunta Maina Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats Journal of Advanced Veterinary and Animal Research Field isolate; Mixed infections; Pathogenicity; Parasitemia; Relapsed isolate |
| title | Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats |
| title_full | Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats |
| title_fullStr | Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats |
| title_full_unstemmed | Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats |
| title_short | Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats |
| title_sort | comparison of pathogenicity of relapsed field and mixed isolates of trypanosoma brucei brucei infections in rats |
| topic | Field isolate; Mixed infections; Pathogenicity; Parasitemia; Relapsed isolate |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=229316 |
| work_keys_str_mv | AT tobiasnniaegbenwiyi comparisonofpathogenicityofrelapsedfieldandmixedisolatesoftrypanosomabruceibruceiinfectionsinrats AT ephraimigwenagu comparisonofpathogenicityofrelapsedfieldandmixedisolatesoftrypanosomabruceibruceiinfectionsinrats AT anastasiatheresanwaosu comparisonofpathogenicityofrelapsedfieldandmixedisolatesoftrypanosomabruceibruceiinfectionsinrats AT meshachmauntamaina comparisonofpathogenicityofrelapsedfieldandmixedisolatesoftrypanosomabruceibruceiinfectionsinrats |