Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i>
<i>Vibrio</i> species are widely distributed and can be potentially pathogenic to aquatic organisms. In this study, we isolated <i>Vibrio</i> spp. from environmental samples (seawater, sediment, and fish swabs) collected over a three-year period from a fish farm in Mali Ston...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/11/2159 |
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| author | Damir Kapetanović Irena Vardić Smrzlić Ana Gavrilović Jurica Jug-Dujaković Lorena Perić Snježana Kazazić Tea Mišić Radić Anamarija Kolda Milan Čanković Jakov Žunić Eddy Listeš Darija Vukić Lušić Atle Lillehaug Semir Lončarević Kristina Pikelj Brigita Hengl Dražen Knežević Mansour El-Matbouli |
| author_facet | Damir Kapetanović Irena Vardić Smrzlić Ana Gavrilović Jurica Jug-Dujaković Lorena Perić Snježana Kazazić Tea Mišić Radić Anamarija Kolda Milan Čanković Jakov Žunić Eddy Listeš Darija Vukić Lušić Atle Lillehaug Semir Lončarević Kristina Pikelj Brigita Hengl Dražen Knežević Mansour El-Matbouli |
| author_sort | Damir Kapetanović |
| collection | DOAJ |
| description | <i>Vibrio</i> species are widely distributed and can be potentially pathogenic to aquatic organisms. In this study, we isolated <i>Vibrio</i> spp. from environmental samples (seawater, sediment, and fish swabs) collected over a three-year period from a fish farm in Mali Ston Bay in the Adriatic Sea, Croatia, and assess their distribution. A total of 48 seawater samples and 12 sediment samples, as well as gill and skin swabs from 110 farmed European seabass, were analysed for the presence of <i>Vibrio</i>. <i>Vibrio</i> strains were identified to the species level by MALDI TOF MS. The analysis revealed that <i>V</i>. <i>alginolyticus</i> was the predominant species in European seabass, followed by <i>V</i>. <i>anguillarum</i>. <i>V</i>. <i>alginolyticus</i> was isolated from the sediments, along with <i>V</i>. <i>gigantis</i> and <i>V</i>. <i>pomeroyi</i>, while <i>V</i>. <i>chagasii</i>, <i>V</i>. <i>cyclitrophicus</i>, <i>V</i>. <i>fortis</i>, <i>V</i>. <i>gigantis</i>, <i>V</i>. <i>harveyi</i>, <i>V</i>. <i>pelagius</i>, and <i>V</i>. <i>pomeroyi</i> were isolated from seawater. <i>V</i>. <i>anguillarum</i> was isolated only twice during two different spring seasons, once from a diseased sea bass and the second time from a healthy sea bass. We analysed these two isolates and found that they differ both genetically and in terms of resistance to antibiotics. Our results confirm the seasonality of vibriosis incidence and the presence of the pathogenic <i>V</i>. <i>anguillarum</i>, which increases the risk of vibriosis. |
| first_indexed | 2024-03-09T18:50:07Z |
| format | Article |
| id | doaj.art-956c3f25d54a45fa999f24aace734e04 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T18:50:07Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-956c3f25d54a45fa999f24aace734e042023-11-24T05:56:43ZengMDPI AGMicroorganisms2076-26072022-10-011011215910.3390/microorganisms10112159Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i>Damir Kapetanović0Irena Vardić Smrzlić1Ana Gavrilović2Jurica Jug-Dujaković3Lorena Perić4Snježana Kazazić5Tea Mišić Radić6Anamarija Kolda7Milan Čanković8Jakov Žunić9Eddy Listeš10Darija Vukić Lušić11Atle Lillehaug12Semir Lončarević13Kristina Pikelj14Brigita Hengl15Dražen Knežević16Mansour El-Matbouli17Ruđer Bošković Institute, 10000 Zagreb, CroatiaRuđer Bošković Institute, 10000 Zagreb, CroatiaFaculty of Agriculture, University of Zagreb, 10000 Zagreb, CroatiaSustainable Aquaculture Systems, Inc., Frenchtown, NJ 08825, USARuđer Bošković Institute, 10000 Zagreb, CroatiaRuđer Bošković Institute, 10000 Zagreb, CroatiaRuđer Bošković Institute, 10000 Zagreb, CroatiaRuđer Bošković Institute, 10000 Zagreb, CroatiaRuđer Bošković Institute, 10000 Zagreb, CroatiaVodovod d.o.o., 23000 Zadar, CroatiaCroatian Veterinary Institute, Regional Veterinary Institute Split, 21000 Split, CroatiaFaculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaNorwegian Veterinary Institute, 1433 Ås, NorwayNorwegian Veterinary Institute, 1433 Ås, NorwayFaculty of Science, University of Zagreb, 10000 Zagreb, CroatiaCroatian Agency for Agriculture and Food, 31000 Osijek, CroatiaCroatian Agency for Agriculture and Food, 31000 Osijek, CroatiaDepartment for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, 1230 Wien, Austria<i>Vibrio</i> species are widely distributed and can be potentially pathogenic to aquatic organisms. In this study, we isolated <i>Vibrio</i> spp. from environmental samples (seawater, sediment, and fish swabs) collected over a three-year period from a fish farm in Mali Ston Bay in the Adriatic Sea, Croatia, and assess their distribution. A total of 48 seawater samples and 12 sediment samples, as well as gill and skin swabs from 110 farmed European seabass, were analysed for the presence of <i>Vibrio</i>. <i>Vibrio</i> strains were identified to the species level by MALDI TOF MS. The analysis revealed that <i>V</i>. <i>alginolyticus</i> was the predominant species in European seabass, followed by <i>V</i>. <i>anguillarum</i>. <i>V</i>. <i>alginolyticus</i> was isolated from the sediments, along with <i>V</i>. <i>gigantis</i> and <i>V</i>. <i>pomeroyi</i>, while <i>V</i>. <i>chagasii</i>, <i>V</i>. <i>cyclitrophicus</i>, <i>V</i>. <i>fortis</i>, <i>V</i>. <i>gigantis</i>, <i>V</i>. <i>harveyi</i>, <i>V</i>. <i>pelagius</i>, and <i>V</i>. <i>pomeroyi</i> were isolated from seawater. <i>V</i>. <i>anguillarum</i> was isolated only twice during two different spring seasons, once from a diseased sea bass and the second time from a healthy sea bass. We analysed these two isolates and found that they differ both genetically and in terms of resistance to antibiotics. Our results confirm the seasonality of vibriosis incidence and the presence of the pathogenic <i>V</i>. <i>anguillarum</i>, which increases the risk of vibriosis.https://www.mdpi.com/2076-2607/10/11/2159<i>Vibrio</i>vibriosiswater qualityseabassfish farmMali Ston Bay |
| spellingShingle | Damir Kapetanović Irena Vardić Smrzlić Ana Gavrilović Jurica Jug-Dujaković Lorena Perić Snježana Kazazić Tea Mišić Radić Anamarija Kolda Milan Čanković Jakov Žunić Eddy Listeš Darija Vukić Lušić Atle Lillehaug Semir Lončarević Kristina Pikelj Brigita Hengl Dražen Knežević Mansour El-Matbouli Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i> Microorganisms <i>Vibrio</i> vibriosis water quality seabass fish farm Mali Ston Bay |
| title | Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i> |
| title_full | Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i> |
| title_fullStr | Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i> |
| title_full_unstemmed | Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i> |
| title_short | Characterization of <i>Vibrio</i> Populations from Cultured European Seabass and the Surrounding Marine Environment with Emphasis on <i>V</i>. <i>anguillarum</i> |
| title_sort | characterization of i vibrio i populations from cultured european seabass and the surrounding marine environment with emphasis on i v i i anguillarum i |
| topic | <i>Vibrio</i> vibriosis water quality seabass fish farm Mali Ston Bay |
| url | https://www.mdpi.com/2076-2607/10/11/2159 |
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