Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis
Streptococcus uberis is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investig...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2022-03-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202203-0002_occurrence-of-virulence-associated-genes-in-streptococcus-uberis-and-streptococcus-parauberis-isolated-from-bov.php |
| _version_ | 1828006484683784192 |
|---|---|
| author | M Zouharova K Nedbalcova P Slama J Bzdil M Masarikova J Matiasovic |
| author_facet | M Zouharova K Nedbalcova P Slama J Bzdil M Masarikova J Matiasovic |
| author_sort | M Zouharova |
| collection | DOAJ |
| description | Streptococcus uberis is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in S. uberis isolated from subclinical or clinical mastitis and its closely related species Streptococcus parauberis in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at least four virulence genes and most isolates carried eight out of ten virulence genes. All S. uberis isolates were positive for the oppF, gapC and sua genes. Among the most prevalent virulence-associated genes skc (98%) and pauA (97%) were also found. The hasA and hasB genes were always present together in 94% of the isolates. The genes cfu and lbp were detected in 6% and 2%, respectively. In the S. uberis isolates, 14 different virulence gene profiles were observed. The most frequent profile was hasA+ hasB+ sua+ skc+ pauA+ gapC+ oppF with variable hasC, observed in 86% of the tested isolates, occurring in 127 out of 135 farms. S. parauberis was identified very sporadically and, although it is closely related to S. uberis, only a rare occurrence of the examined virulence-associated genes was found. |
| first_indexed | 2024-04-10T07:57:43Z |
| format | Article |
| id | doaj.art-956ed3dcdff041c48b948f3ea0052361 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:57:43Z |
| publishDate | 2022-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-956ed3dcdff041c48b948f3ea00523612023-02-23T03:51:11ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922022-03-0167312313010.17221/95/2021-VETMEDvet-202203-0002Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitisM Zouharova0K Nedbalcova1P Slama2J Bzdil3M Masarikova4J Matiasovic5Infectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Brno, Czech RepublicInfectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech RepublicMicrobiologist and Practical Veterinarian, Ptácy s.r.o., Valašská Bystřice, Czech RepublicDepartment of Infectious Diseases and Microbiology, University of Veterinary Sciences, Brno, Czech RepublicInfectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Brno, Czech RepublicStreptococcus uberis is one of the most important mastitis-causing pathogens. Although the pathogenesis and virulence factors required for the intramammary infection development are not yet well established, several putative virulence-associated genes have been described. This work aimed to investigate the presence of ten known and putative virulence-associated genes in S. uberis isolated from subclinical or clinical mastitis and its closely related species Streptococcus parauberis in 135 dairy farms in the Czech Republic. The PCR analysis detected that all the examined isolates possessed at least four virulence genes and most isolates carried eight out of ten virulence genes. All S. uberis isolates were positive for the oppF, gapC and sua genes. Among the most prevalent virulence-associated genes skc (98%) and pauA (97%) were also found. The hasA and hasB genes were always present together in 94% of the isolates. The genes cfu and lbp were detected in 6% and 2%, respectively. In the S. uberis isolates, 14 different virulence gene profiles were observed. The most frequent profile was hasA+ hasB+ sua+ skc+ pauA+ gapC+ oppF with variable hasC, observed in 86% of the tested isolates, occurring in 127 out of 135 farms. S. parauberis was identified very sporadically and, although it is closely related to S. uberis, only a rare occurrence of the examined virulence-associated genes was found.https://vetmed.agriculturejournals.cz/artkey/vet-202203-0002_occurrence-of-virulence-associated-genes-in-streptococcus-uberis-and-streptococcus-parauberis-isolated-from-bov.phpcowsintramammary infectionsmammary glandpathogensvirulence factors |
| spellingShingle | M Zouharova K Nedbalcova P Slama J Bzdil M Masarikova J Matiasovic Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis Veterinární Medicína cows intramammary infections mammary gland pathogens virulence factors |
| title | Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis |
| title_full | Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis |
| title_fullStr | Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis |
| title_full_unstemmed | Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis |
| title_short | Occurrence of virulence-associated genes in Streptococcus uberis and Streptococcus parauberis isolated from bovine mastitis |
| title_sort | occurrence of virulence associated genes in streptococcus uberis and streptococcus parauberis isolated from bovine mastitis |
| topic | cows intramammary infections mammary gland pathogens virulence factors |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202203-0002_occurrence-of-virulence-associated-genes-in-streptococcus-uberis-and-streptococcus-parauberis-isolated-from-bov.php |
| work_keys_str_mv | AT mzouharova occurrenceofvirulenceassociatedgenesinstreptococcusuberisandstreptococcusparauberisisolatedfrombovinemastitis AT knedbalcova occurrenceofvirulenceassociatedgenesinstreptococcusuberisandstreptococcusparauberisisolatedfrombovinemastitis AT pslama occurrenceofvirulenceassociatedgenesinstreptococcusuberisandstreptococcusparauberisisolatedfrombovinemastitis AT jbzdil occurrenceofvirulenceassociatedgenesinstreptococcusuberisandstreptococcusparauberisisolatedfrombovinemastitis AT mmasarikova occurrenceofvirulenceassociatedgenesinstreptococcusuberisandstreptococcusparauberisisolatedfrombovinemastitis AT jmatiasovic occurrenceofvirulenceassociatedgenesinstreptococcusuberisandstreptococcusparauberisisolatedfrombovinemastitis |