Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation
GADD34 and GADD45 factors play an important role in suppression of malignant tumor growth. It was shown that blockade of both enzymatic functions of sensor and signaling enzyme ERN1 (from endoplasmic reticulum to nuclei-1), the major component of endoplasmic reticulum stress signaling, decreases the...
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| Format: | Article |
| Language: | English |
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Taras Shevchenko National University of Kyiv
2016-11-01
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| Series: | Vìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ |
| Subjects: | |
| Online Access: | http://biovestnik.com/index.php/biology/article/view/100 |
| _version_ | 1827842670531182592 |
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| author | I. Kryvdiuk T. Bakalets O. Riabovol D. Minchenko |
| author_facet | I. Kryvdiuk T. Bakalets O. Riabovol D. Minchenko |
| author_sort | I. Kryvdiuk |
| collection | DOAJ |
| description | GADD34 and GADD45 factors play an important role in suppression of malignant tumor growth. It was shown that blockade of both enzymatic functions of sensor and signaling enzyme ERN1 (from endoplasmic reticulum to nuclei-1), the major component of endoplasmic reticulum stress signaling, decreases the expression level of genes GADD34 and GADD45A and increases the GADD45B gene expression in U87 glioma cell. The increase of GADD45B and decrease of GADD34 and GADD45A gene expressions in glioma cells with ERN1 signaling enzyme loss of function is possibly participated in suppression of these cells proliferation. It was shown that, that glucose deprivation condition leads to enhance the expression of GADD34, GADD45A and GADD45В genes in both types of glioma cells, but complete blockade of ERN1 enzyme function modifies the effect of glucose deprivation condition on the expression level all of these genes. Thus, results of this investigation demonstrated that the expression of GADD34, GADD45A and GADD45В genes in U87 glioma cells is dependent from signaling enzyme ERN1 and is changed by glucose deprivation condition, but its effect depends from ERN1 signaling enzyme function. |
| first_indexed | 2024-03-12T08:13:02Z |
| format | Article |
| id | doaj.art-95753b48f65f4ed9a6d6a8c427e2e7c7 |
| institution | Directory Open Access Journal |
| issn | 1728-2748 2308-8036 |
| language | English |
| last_indexed | 2024-03-12T08:13:02Z |
| publishDate | 2016-11-01 |
| publisher | Taras Shevchenko National University of Kyiv |
| record_format | Article |
| series | Vìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ |
| spelling | doaj.art-95753b48f65f4ed9a6d6a8c427e2e7c72023-09-02T19:06:26ZengTaras Shevchenko National University of KyivVìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ1728-27482308-80362016-11-01661283291Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivationI. Kryvdiuk0T. Bakalets1O. Riabovol2D. Minchenko3Palladin instityte of biochemistry of National Academy of Sciences of Ukraine NASU, KyivPalladin instityte of biochemistry of National Academy of Sciences of Ukraine NASU, KyivPalladin instityte of biochemistry of National Academy of Sciences of Ukraine NASU, KyivPalladin instityte of biochemistry of National Academy of Sciences of Ukraine NASU, KyivGADD34 and GADD45 factors play an important role in suppression of malignant tumor growth. It was shown that blockade of both enzymatic functions of sensor and signaling enzyme ERN1 (from endoplasmic reticulum to nuclei-1), the major component of endoplasmic reticulum stress signaling, decreases the expression level of genes GADD34 and GADD45A and increases the GADD45B gene expression in U87 glioma cell. The increase of GADD45B and decrease of GADD34 and GADD45A gene expressions in glioma cells with ERN1 signaling enzyme loss of function is possibly participated in suppression of these cells proliferation. It was shown that, that glucose deprivation condition leads to enhance the expression of GADD34, GADD45A and GADD45В genes in both types of glioma cells, but complete blockade of ERN1 enzyme function modifies the effect of glucose deprivation condition on the expression level all of these genes. Thus, results of this investigation demonstrated that the expression of GADD34, GADD45A and GADD45В genes in U87 glioma cells is dependent from signaling enzyme ERN1 and is changed by glucose deprivation condition, but its effect depends from ERN1 signaling enzyme function.http://biovestnik.com/index.php/biology/article/view/100GADD34, GADD45, glucose, glioma cells |
| spellingShingle | I. Kryvdiuk T. Bakalets O. Riabovol D. Minchenko Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation Vìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ GADD34, GADD45, glucose, glioma cells |
| title | Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation |
| title_full | Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation |
| title_fullStr | Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation |
| title_full_unstemmed | Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation |
| title_short | Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation |
| title_sort | expression of gadd34 and gsdd45 mrna in u87 glioma cells effect of ern1 signaling enzyme and glucose deprivation |
| topic | GADD34, GADD45, glucose, glioma cells |
| url | http://biovestnik.com/index.php/biology/article/view/100 |
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