Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection
Abstract Objectives The pediatric urinary tract infection (UTI) often remains under-diagnosed or neglected owing to non-specific clinical presentations, patients failing to describe the actual situation and of clinical practice in diagnosis. The study was aimed to determine the etiologies of UTI in...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-019-4875-y |
| _version_ | 1819052022021750784 |
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| author | Januka Thapaliya Priyatam Khadka Shovana Thapa Chenu Gongal |
| author_facet | Januka Thapaliya Priyatam Khadka Shovana Thapa Chenu Gongal |
| author_sort | Januka Thapaliya |
| collection | DOAJ |
| description | Abstract Objectives The pediatric urinary tract infection (UTI) often remains under-diagnosed or neglected owing to non-specific clinical presentations, patients failing to describe the actual situation and of clinical practice in diagnosis. The study was aimed to determine the etiologies of UTI in children with enhanced quantitative urine culture (EQUC) technique. Results Of enrolled 570 pediatric urine samples, the significant growth positivity was higher in EQUC 92 (16.15%) compared to standard urine culture (SUC) 73 (12.80%) technique. 20.6% of the significant isolates as detected with EQUC were missed on the SUC technique. The age group, in range 1–4 years, was more prone to the infection, where E. coli was the commonest pathogen. EQUC detected, probably all isolates, contributing UTI i.e. multidrug-resistant (MDR), extensive drug-resistant (XDR), and extended-spectrum β-lactamase (ESBL) producers, as some of them skipped on the SUC technique. Of total organisms isolated from EQUC, 46% were ESBL producer, 56.5% were MDR, and 1.4% were XDR. However, 40.5% ESBL, 44% MDR but no XDR detected on SUC. Hence a simple modification on conventional culture protocol could be a crucial modification for the detection of etiologies, contributing UTI, and hence to reduce inapt antimicrobial burden. |
| first_indexed | 2024-12-21T12:13:13Z |
| format | Article |
| id | doaj.art-95762693d81346cbac0ef7fc84320d45 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-21T12:13:13Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-95762693d81346cbac0ef7fc84320d452022-12-21T19:04:31ZengBMCBMC Research Notes1756-05002020-01-011311610.1186/s13104-019-4875-yEnhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infectionJanuka Thapaliya0Priyatam Khadka1Shovana Thapa2Chenu Gongal3Tri-Chandra Multiple Campus, Tribhuvan UniversityTri-Chandra Multiple Campus, Tribhuvan UniversityInternational Friendship Children’s HospitalTri-Chandra Multiple Campus, Tribhuvan UniversityAbstract Objectives The pediatric urinary tract infection (UTI) often remains under-diagnosed or neglected owing to non-specific clinical presentations, patients failing to describe the actual situation and of clinical practice in diagnosis. The study was aimed to determine the etiologies of UTI in children with enhanced quantitative urine culture (EQUC) technique. Results Of enrolled 570 pediatric urine samples, the significant growth positivity was higher in EQUC 92 (16.15%) compared to standard urine culture (SUC) 73 (12.80%) technique. 20.6% of the significant isolates as detected with EQUC were missed on the SUC technique. The age group, in range 1–4 years, was more prone to the infection, where E. coli was the commonest pathogen. EQUC detected, probably all isolates, contributing UTI i.e. multidrug-resistant (MDR), extensive drug-resistant (XDR), and extended-spectrum β-lactamase (ESBL) producers, as some of them skipped on the SUC technique. Of total organisms isolated from EQUC, 46% were ESBL producer, 56.5% were MDR, and 1.4% were XDR. However, 40.5% ESBL, 44% MDR but no XDR detected on SUC. Hence a simple modification on conventional culture protocol could be a crucial modification for the detection of etiologies, contributing UTI, and hence to reduce inapt antimicrobial burden.https://doi.org/10.1186/s13104-019-4875-yUTIEQUCUropathogensChildrenESBLMDR |
| spellingShingle | Januka Thapaliya Priyatam Khadka Shovana Thapa Chenu Gongal Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection BMC Research Notes UTI EQUC Uropathogens Children ESBL MDR |
| title | Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection |
| title_full | Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection |
| title_fullStr | Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection |
| title_full_unstemmed | Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection |
| title_short | Enhanced quantitative urine culture technique, a slight modification, in detecting under-diagnosed pediatric urinary tract infection |
| title_sort | enhanced quantitative urine culture technique a slight modification in detecting under diagnosed pediatric urinary tract infection |
| topic | UTI EQUC Uropathogens Children ESBL MDR |
| url | https://doi.org/10.1186/s13104-019-4875-y |
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