Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids
Linoleic acid (18 : 2, n-6) and α-linolenic acid (18 : 3, n-3) are polyunsaturated fatty acids (PUFAs), which are essential for mammalian health, development and growth. However, the majority of mammals, including humans, are incapable of synthesizing n-6 and n-3 PUFAs. Mammals must obtain n-6 and n...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2019-10-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.190140 |
| _version_ | 1828415353864060928 |
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| author | Lishuang Song Lei Yang Jiapeng Wang Xuefei Liu Lige Bai Anqi Di Guangpeng Li |
| author_facet | Lishuang Song Lei Yang Jiapeng Wang Xuefei Liu Lige Bai Anqi Di Guangpeng Li |
| author_sort | Lishuang Song |
| collection | DOAJ |
| description | Linoleic acid (18 : 2, n-6) and α-linolenic acid (18 : 3, n-3) are polyunsaturated fatty acids (PUFAs), which are essential for mammalian health, development and growth. However, the majority of mammals, including humans, are incapable of synthesizing n-6 and n-3 PUFAs. Mammals must obtain n-6 and n-3 PUFAs from their diet. Fatty acid desaturase (Fad) plays a critical role in plant PUFA biosynthesis. Therefore, we generated plant-derived Fad3 single and Fad2–Fad3 double transgenic mice. Compared with wild-type mice, we found that PUFA levels were greatly increased in the single and double transgenic mice by measuring PUFA levels. Moreover, the concentration of n-6 and n-3 PUFAs in the Fad2–Fad3 double transgenic mice were greater than in the Fad3 single transgenic mice. These results demonstrate that the plant-derived Fad2 and Fad3 genes can be expressed in mammals. To clarify the mechanism for Fad2 and Fad3 genes in transgenic mice, we measured the PUFAs synthesis-related genes. Compared with wild-type mice, these Fad transgenic mice have their own n-3 and n-6 PUFAs biosynthetic pathways. Thus, we have established a simple and efficient method for in vivo synthesis of PUFAs. |
| first_indexed | 2024-12-10T13:43:28Z |
| format | Article |
| id | doaj.art-958159b212754845b06b86ad190d98c2 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-10T13:43:28Z |
| publishDate | 2019-10-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-958159b212754845b06b86ad190d98c22022-12-22T01:46:34ZengThe Royal SocietyOpen Biology2046-24412019-10-0191010.1098/rsob.190140190140Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acidsLishuang SongLei YangJiapeng WangXuefei LiuLige BaiAnqi DiGuangpeng LiLinoleic acid (18 : 2, n-6) and α-linolenic acid (18 : 3, n-3) are polyunsaturated fatty acids (PUFAs), which are essential for mammalian health, development and growth. However, the majority of mammals, including humans, are incapable of synthesizing n-6 and n-3 PUFAs. Mammals must obtain n-6 and n-3 PUFAs from their diet. Fatty acid desaturase (Fad) plays a critical role in plant PUFA biosynthesis. Therefore, we generated plant-derived Fad3 single and Fad2–Fad3 double transgenic mice. Compared with wild-type mice, we found that PUFA levels were greatly increased in the single and double transgenic mice by measuring PUFA levels. Moreover, the concentration of n-6 and n-3 PUFAs in the Fad2–Fad3 double transgenic mice were greater than in the Fad3 single transgenic mice. These results demonstrate that the plant-derived Fad2 and Fad3 genes can be expressed in mammals. To clarify the mechanism for Fad2 and Fad3 genes in transgenic mice, we measured the PUFAs synthesis-related genes. Compared with wild-type mice, these Fad transgenic mice have their own n-3 and n-6 PUFAs biosynthetic pathways. Thus, we have established a simple and efficient method for in vivo synthesis of PUFAs.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.190140polyunsaturated fatty acids (pufas)n-3/6 pufasfatty acid desaturase (fad)fad2/3transgenic mice |
| spellingShingle | Lishuang Song Lei Yang Jiapeng Wang Xuefei Liu Lige Bai Anqi Di Guangpeng Li Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids Open Biology polyunsaturated fatty acids (pufas) n-3/6 pufas fatty acid desaturase (fad) fad2/3 transgenic mice |
| title | Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids |
| title_full | Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids |
| title_fullStr | Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids |
| title_full_unstemmed | Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids |
| title_short | Generation of Fad2 and Fad3 transgenic mice that produce n-6 and n-3 polyunsaturated fatty acids |
| title_sort | generation of fad2 and fad3 transgenic mice that produce n 6 and n 3 polyunsaturated fatty acids |
| topic | polyunsaturated fatty acids (pufas) n-3/6 pufas fatty acid desaturase (fad) fad2/3 transgenic mice |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.190140 |
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