A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves
Summary: A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672100441X |
| _version_ | 1819243010870738944 |
|---|---|
| author | Hongmin Yun Kira L. Lathrop Anthony J. St. Leger |
| author_facet | Hongmin Yun Kira L. Lathrop Anthony J. St. Leger |
| author_sort | Hongmin Yun |
| collection | DOAJ |
| description | Summary: A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohistochemistry protocol that facilitates evaluation of nerve architecture throughout the mouse cornea. The fixation step in this protocol allows for reliable detection of nerve structures within the cornea and likely other tissues.For complete details on the use and execution of this protocol, please refer to Yun et al, (2020). |
| first_indexed | 2024-12-23T14:48:54Z |
| format | Article |
| id | doaj.art-95818de75a4d4cdeb99f0bda74665974 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-23T14:48:54Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-95818de75a4d4cdeb99f0bda746659742022-12-21T17:43:01ZengElsevierSTAR Protocols2666-16672021-09-0123100734A whole-mount immunohistochemistry protocol for detection of mouse corneal nervesHongmin Yun0Kira L. Lathrop1Anthony J. St. Leger2Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Corresponding authorDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15213, USA; Corresponding authorDepartment of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA; Corresponding authorSummary: A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohistochemistry protocol that facilitates evaluation of nerve architecture throughout the mouse cornea. The fixation step in this protocol allows for reliable detection of nerve structures within the cornea and likely other tissues.For complete details on the use and execution of this protocol, please refer to Yun et al, (2020).http://www.sciencedirect.com/science/article/pii/S266616672100441XMicroscopyNeuroscience |
| spellingShingle | Hongmin Yun Kira L. Lathrop Anthony J. St. Leger A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves STAR Protocols Microscopy Neuroscience |
| title | A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves |
| title_full | A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves |
| title_fullStr | A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves |
| title_full_unstemmed | A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves |
| title_short | A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves |
| title_sort | whole mount immunohistochemistry protocol for detection of mouse corneal nerves |
| topic | Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S266616672100441X |
| work_keys_str_mv | AT hongminyun awholemountimmunohistochemistryprotocolfordetectionofmousecornealnerves AT kirallathrop awholemountimmunohistochemistryprotocolfordetectionofmousecornealnerves AT anthonyjstleger awholemountimmunohistochemistryprotocolfordetectionofmousecornealnerves AT hongminyun wholemountimmunohistochemistryprotocolfordetectionofmousecornealnerves AT kirallathrop wholemountimmunohistochemistryprotocolfordetectionofmousecornealnerves AT anthonyjstleger wholemountimmunohistochemistryprotocolfordetectionofmousecornealnerves |