Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes
Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was repo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Korean Society of Animal Reproduction and Biotechnology
2021-12-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | https://www.e-jarb.org/journal/view.html?uid=2598&vmd=Full |
| _version_ | 1818974648463785984 |
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| author | Junyoung Kim Youngwook Jung Heejun Jung Muhammad Shakeel Minjung Yoon |
| author_facet | Junyoung Kim Youngwook Jung Heejun Jung Muhammad Shakeel Minjung Yoon |
| author_sort | Junyoung Kim |
| collection | DOAJ |
| description | Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcriptionpolymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes. |
| first_indexed | 2024-12-20T15:43:24Z |
| format | Article |
| id | doaj.art-95828a8b95b74e3192abe7460cf1e970 |
| institution | Directory Open Access Journal |
| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-20T15:43:24Z |
| publishDate | 2021-12-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-95828a8b95b74e3192abe7460cf1e9702022-12-21T19:35:06ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632021-12-0136429229810.12750/JARB.36.4.292Olfactory receptor (OR7D4 and OR1I1) expression in stallion testesJunyoung Kim0https://orcid.org/0000-0003-0711-5479Youngwook Jung1https://orcid.org/0000-0002-7829-0349Heejun Jung 2https://orcid.org/0000-0001-9608-1412Muhammad Shakeel 3https://orcid.org/0000-0001-8436-5741Minjung Yoon4https://orcid.org/0000-0001-9112-1796Department of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, KoreaOlfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcriptionpolymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes.https://www.e-jarb.org/journal/view.html?uid=2598&vmd=Fullolfactory receptoror1i1or7d4stalliontestis |
| spellingShingle | Junyoung Kim Youngwook Jung Heejun Jung Muhammad Shakeel Minjung Yoon Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes Journal of Animal Reproduction and Biotechnology olfactory receptor or1i1 or7d4 stallion testis |
| title | Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes |
| title_full | Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes |
| title_fullStr | Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes |
| title_full_unstemmed | Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes |
| title_short | Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes |
| title_sort | olfactory receptor or7d4 and or1i1 expression in stallion testes |
| topic | olfactory receptor or1i1 or7d4 stallion testis |
| url | https://www.e-jarb.org/journal/view.html?uid=2598&vmd=Full |
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