A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice
Staphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1088501/full |
| _version_ | 1797948189301538816 |
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| author | Jingjing Sun Jingjing Sun Xuansheng Lin Yige He Yige He Baozhong Zhang Nan Zhou Nan Zhou Jian-dong Huang Jian-dong Huang Jian-dong Huang Jian-dong Huang |
| author_facet | Jingjing Sun Jingjing Sun Xuansheng Lin Yige He Yige He Baozhong Zhang Nan Zhou Nan Zhou Jian-dong Huang Jian-dong Huang Jian-dong Huang Jian-dong Huang |
| author_sort | Jingjing Sun |
| collection | DOAJ |
| description | Staphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasion strategies developed by S. aureus is thus crucial for its infection control. In this study, we exploited the SpyCatcher-SpyTag system to engineer bacterial outer membrane vesicles (OMVs) for the development of a multitargeting S. aureus click vaccine. We decorated OMVs with surface exposed SpyCatcher via a truncated OmpA(a.a 1-155)-SpyCatcher fusion. The engineered OMVs can flexibly bind with various SpyTag-fused S. aureus antigens to generate an OMV-based click vaccine. Compared with antigens mixed with alum adjuvant, the click vaccine simultaneously induced more potent antigen-specific humoral and Th1-based cellular immune response, which afforded protection against S. aureus Newman lethal challenge in a mouse model. Our study provided a flexible and versatile click vaccine strategy with the potential for fighting against emerging S. aureus clinical isolates. |
| first_indexed | 2024-04-10T21:40:32Z |
| format | Article |
| id | doaj.art-9582ad7051174ce2b48e8b055f610848 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T21:40:32Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-9582ad7051174ce2b48e8b055f6108482023-01-19T05:59:18ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-01-011410.3389/fimmu.2023.10885011088501A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in miceJingjing Sun0Jingjing Sun1Xuansheng Lin2Yige He3Yige He4Baozhong Zhang5Nan Zhou6Nan Zhou7Jian-dong Huang8Jian-dong Huang9Jian-dong Huang10Jian-dong Huang11CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, ChinaSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaDepartment of Clinical Oncology, Shenzhen Key Laboratory for Cancer Metastasis and Personalized Therapy, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, ChinaGuangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen University, Guangzhou, ChinaStaphylococcus aureus infection is a severe public health concern with the growing number of multidrug-resistant strains. S. aureus can circumvent the defense mechanisms of host immunity with the aid of multiple virulence factors. An efficacious multicomponent vaccine targeting diverse immune evasion strategies developed by S. aureus is thus crucial for its infection control. In this study, we exploited the SpyCatcher-SpyTag system to engineer bacterial outer membrane vesicles (OMVs) for the development of a multitargeting S. aureus click vaccine. We decorated OMVs with surface exposed SpyCatcher via a truncated OmpA(a.a 1-155)-SpyCatcher fusion. The engineered OMVs can flexibly bind with various SpyTag-fused S. aureus antigens to generate an OMV-based click vaccine. Compared with antigens mixed with alum adjuvant, the click vaccine simultaneously induced more potent antigen-specific humoral and Th1-based cellular immune response, which afforded protection against S. aureus Newman lethal challenge in a mouse model. Our study provided a flexible and versatile click vaccine strategy with the potential for fighting against emerging S. aureus clinical isolates.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1088501/fullouter membrane vesiclesStaphylococcus aureus vaccineSpyCatcher-SpyTag‘click’ displayflexible antigen displaymulti-targeting vaccine |
| spellingShingle | Jingjing Sun Jingjing Sun Xuansheng Lin Yige He Yige He Baozhong Zhang Nan Zhou Nan Zhou Jian-dong Huang Jian-dong Huang Jian-dong Huang Jian-dong Huang A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice Frontiers in Immunology outer membrane vesicles Staphylococcus aureus vaccine SpyCatcher-SpyTag ‘click’ display flexible antigen display multi-targeting vaccine |
| title | A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice |
| title_full | A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice |
| title_fullStr | A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice |
| title_full_unstemmed | A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice |
| title_short | A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice |
| title_sort | bacterial outer membrane vesicle based click vaccine elicits potent immune response against staphylococcus aureus in mice |
| topic | outer membrane vesicles Staphylococcus aureus vaccine SpyCatcher-SpyTag ‘click’ display flexible antigen display multi-targeting vaccine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1088501/full |
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