Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers
Red blood cells (RBCs) are able to interact and communicate with endothelial cells (ECs). Under some pathological or even normal conditions, the adhesion of RBCs to the endothelium can be observed. Presently, the mechanisms and many aspects of the interaction between RBCs and ECs are not fully under...
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2021-09-01
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| Series: | Journal of Innovative Optical Health Sciences |
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| Online Access: | http://www.worldscientific.com/doi/epdf/10.1142/S1793545821420050 |
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| author | Arseniy A. Kapkov Alexey N. Semenov Petr B. Ermolinskiy Andrei E. Lugovtsov Alexander V. Priezzhev |
| author_facet | Arseniy A. Kapkov Alexey N. Semenov Petr B. Ermolinskiy Andrei E. Lugovtsov Alexander V. Priezzhev |
| author_sort | Arseniy A. Kapkov |
| collection | DOAJ |
| description | Red blood cells (RBCs) are able to interact and communicate with endothelial cells (ECs). Under some pathological or even normal conditions, the adhesion of RBCs to the endothelium can be observed. Presently, the mechanisms and many aspects of the interaction between RBCs and ECs are not fully understood. In this work, we considered the interaction of single RBCs with single ECs in vitro aiming to quantitatively determine the force of this interaction using laser tweezers. Measurements were performed under different concentrations of proaggregant macromolecules and in the presence or absence of tumor necrosis factor (TNF-α) activating the ECs. We have shown that the strength of interaction depends on the concentration of fibrinogen or dextran proaggregant macromolecules in the environment. A nonlinear increase in the force of cells interaction (from 0.4 pN to 21 pN) was observed along with an increase in the fibrinogen concentration (from 3mg/mL to 9mg/mL) in blood plasma, as well as with the addition of dextran macromolecules (from 10mg/mL to 60mg/mL). Dextran with a higher molecular mass (500kDa) enhances the adhesion of RBCs to ECs greater compared to the dextran with a lower molecular mass (70kDa). With the preliminary activation of ECs with TNF-α, the force of interaction increases. Also, the adhesion of echinocytes to EC compared to discocytes is significantly higher. These results may help to better understand the process of interaction between RBCs and ECs. |
| first_indexed | 2024-12-21T03:13:42Z |
| format | Article |
| id | doaj.art-9583cb46d6254cedac5956be729bb936 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-21T03:13:42Z |
| publishDate | 2021-09-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-9583cb46d6254cedac5956be729bb9362022-12-21T19:17:54ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052021-09-011452142005-12142005-1110.1142/S179354582142005010.1142/S1793545821420050Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezersArseniy A. Kapkov0Alexey N. Semenov1Petr B. Ermolinskiy2Andrei E. Lugovtsov3Alexander V. Priezzhev4Faculty of Physics, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, RussiaFaculty of Biology, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, RussiaFaculty of Physics, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, RussiaFaculty of Physics, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, RussiaFaculty of Physics, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, RussiaRed blood cells (RBCs) are able to interact and communicate with endothelial cells (ECs). Under some pathological or even normal conditions, the adhesion of RBCs to the endothelium can be observed. Presently, the mechanisms and many aspects of the interaction between RBCs and ECs are not fully understood. In this work, we considered the interaction of single RBCs with single ECs in vitro aiming to quantitatively determine the force of this interaction using laser tweezers. Measurements were performed under different concentrations of proaggregant macromolecules and in the presence or absence of tumor necrosis factor (TNF-α) activating the ECs. We have shown that the strength of interaction depends on the concentration of fibrinogen or dextran proaggregant macromolecules in the environment. A nonlinear increase in the force of cells interaction (from 0.4 pN to 21 pN) was observed along with an increase in the fibrinogen concentration (from 3mg/mL to 9mg/mL) in blood plasma, as well as with the addition of dextran macromolecules (from 10mg/mL to 60mg/mL). Dextran with a higher molecular mass (500kDa) enhances the adhesion of RBCs to ECs greater compared to the dextran with a lower molecular mass (70kDa). With the preliminary activation of ECs with TNF-α, the force of interaction increases. Also, the adhesion of echinocytes to EC compared to discocytes is significantly higher. These results may help to better understand the process of interaction between RBCs and ECs.http://www.worldscientific.com/doi/epdf/10.1142/S1793545821420050endothelial cellsred blood cellslaser tweezersadhesion |
| spellingShingle | Arseniy A. Kapkov Alexey N. Semenov Petr B. Ermolinskiy Andrei E. Lugovtsov Alexander V. Priezzhev Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers Journal of Innovative Optical Health Sciences endothelial cells red blood cells laser tweezers adhesion |
| title | Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers |
| title_full | Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers |
| title_fullStr | Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers |
| title_full_unstemmed | Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers |
| title_short | Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers |
| title_sort | forces of rbc interaction with single endothelial cells in stationary conditions measurements with laser tweezers |
| topic | endothelial cells red blood cells laser tweezers adhesion |
| url | http://www.worldscientific.com/doi/epdf/10.1142/S1793545821420050 |
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