Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress
Although numerous studies have shown that the hypothalamic–pituitary–adrenal axis plays a vital role in the response to environmental stress by mediating the production of a series of hormones, the mechanism underlying these effects has not been elucidated. This study used proteomics techniques to i...
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MDPI AG
2024-03-01
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| Online Access: | https://www.mdpi.com/2075-1729/14/3/366 |
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| author | Qiu Zhou Yuan Gao Yin Li Huili Xie Xiaoxi Liu Yanhong Yong Youquan Li Zhichao Yu Xingbin Ma Xianghong Ju |
| author_facet | Qiu Zhou Yuan Gao Yin Li Huili Xie Xiaoxi Liu Yanhong Yong Youquan Li Zhichao Yu Xingbin Ma Xianghong Ju |
| author_sort | Qiu Zhou |
| collection | DOAJ |
| description | Although numerous studies have shown that the hypothalamic–pituitary–adrenal axis plays a vital role in the response to environmental stress by mediating the production of a series of hormones, the mechanism underlying these effects has not been elucidated. This study used proteomics techniques to investigate the differentially expressed proteins (DEPs) in the pituitary glands of pigs and to elucidate the potential changes in the immune–neuroendocrine system under heat stress (HS). In total, 2517 peptides corresponding to 205 proteins were detected. A comparison of the expression patterns between HSs and healthy controls revealed 56 DEPs, of which 31 were upregulated and 25 were downregulated. Ingenuity pathway analysis (IPA) was used to reveal the subcellular characteristics, functional pathways, regulatory networks, and upstream regulators of the identified proteins. The results showed that these differentially expressed proteins were involved in intercellular communication, interactions, apoptosis, nervous system development, functions, abnormalities and other functions, and in the regulatory network. Moreover, the upstream regulators of the differentially expressed proteins were mainly transcriptional regulators, hormones, and cytokines. Thus, the functional network and pathway analyses could provide insights into the complexity and dynamics of HS–host interactions and may accelerate our understanding of the mechanisms underlying HS. |
| first_indexed | 2024-04-24T18:05:00Z |
| format | Article |
| id | doaj.art-9584c2ee1a1445e5b8d008f65e5d55b6 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-24T18:05:00Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-9584c2ee1a1445e5b8d008f65e5d55b62024-03-27T13:51:15ZengMDPI AGLife2075-17292024-03-0114336610.3390/life14030366Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat StressQiu Zhou0Yuan Gao1Yin Li2Huili Xie3Xiaoxi Liu4Yanhong Yong5Youquan Li6Zhichao Yu7Xingbin Ma8Xianghong Ju9Marine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaMarine Medical Research and Development Centre, Shenzheng Institute, Guangdong Ocean University, Shenzheng 518120, ChinaAlthough numerous studies have shown that the hypothalamic–pituitary–adrenal axis plays a vital role in the response to environmental stress by mediating the production of a series of hormones, the mechanism underlying these effects has not been elucidated. This study used proteomics techniques to investigate the differentially expressed proteins (DEPs) in the pituitary glands of pigs and to elucidate the potential changes in the immune–neuroendocrine system under heat stress (HS). In total, 2517 peptides corresponding to 205 proteins were detected. A comparison of the expression patterns between HSs and healthy controls revealed 56 DEPs, of which 31 were upregulated and 25 were downregulated. Ingenuity pathway analysis (IPA) was used to reveal the subcellular characteristics, functional pathways, regulatory networks, and upstream regulators of the identified proteins. The results showed that these differentially expressed proteins were involved in intercellular communication, interactions, apoptosis, nervous system development, functions, abnormalities and other functions, and in the regulatory network. Moreover, the upstream regulators of the differentially expressed proteins were mainly transcriptional regulators, hormones, and cytokines. Thus, the functional network and pathway analyses could provide insights into the complexity and dynamics of HS–host interactions and may accelerate our understanding of the mechanisms underlying HS.https://www.mdpi.com/2075-1729/14/3/366heat stressquantitative proteomicspituitary glandfunctional networks |
| spellingShingle | Qiu Zhou Yuan Gao Yin Li Huili Xie Xiaoxi Liu Yanhong Yong Youquan Li Zhichao Yu Xingbin Ma Xianghong Ju Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress Life heat stress quantitative proteomics pituitary gland functional networks |
| title | Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress |
| title_full | Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress |
| title_fullStr | Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress |
| title_full_unstemmed | Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress |
| title_short | Preliminary Proteomic Study of the Porcine Pituitary Gland under Heat Stress |
| title_sort | preliminary proteomic study of the porcine pituitary gland under heat stress |
| topic | heat stress quantitative proteomics pituitary gland functional networks |
| url | https://www.mdpi.com/2075-1729/14/3/366 |
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