Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials

ABSTRACT Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the...

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Bibliographic Details
Main Authors: Victor Vasilevich Revin, Elena Gennadievna Kostina, Nadezhda Victorovna Revina, Vitalina Victorovna Shutova
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2018-08-01
Series:Brazilian Archives of Biology and Technology
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100407&lng=en&tlng=en
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Summary:ABSTRACT Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the maximal growth was found in the medium with 4% glucose. It was found that for the alginate production the optimal nitrogen contents could take from 0.05% yeast extract (carbon: nitrogen ratio 168:1). For the first time we demonstrated possibility the A. vinelandii growth during the cultivation in a medium with molasses (a by-product of sugar production) and the significant polysaccharide production (16.6 g/l) was obtained. It was established, that A. vinelandii culture broth could be used as a biological binder for obtaining the biocomposite materials.
ISSN:1678-4324