Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials
ABSTRACT Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2018-08-01
|
Series: | Brazilian Archives of Biology and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100407&lng=en&tlng=en |
_version_ | 1819262910772281344 |
---|---|
author | Victor Vasilevich Revin Elena Gennadievna Kostina Nadezhda Victorovna Revina Vitalina Victorovna Shutova |
author_facet | Victor Vasilevich Revin Elena Gennadievna Kostina Nadezhda Victorovna Revina Vitalina Victorovna Shutova |
author_sort | Victor Vasilevich Revin |
collection | DOAJ |
description | ABSTRACT Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the maximal growth was found in the medium with 4% glucose. It was found that for the alginate production the optimal nitrogen contents could take from 0.05% yeast extract (carbon: nitrogen ratio 168:1). For the first time we demonstrated possibility the A. vinelandii growth during the cultivation in a medium with molasses (a by-product of sugar production) and the significant polysaccharide production (16.6 g/l) was obtained. It was established, that A. vinelandii culture broth could be used as a biological binder for obtaining the biocomposite materials. |
first_indexed | 2024-12-23T20:05:12Z |
format | Article |
id | doaj.art-958531c3aa754059af14917a0059f2b2 |
institution | Directory Open Access Journal |
issn | 1678-4324 |
language | English |
last_indexed | 2024-12-23T20:05:12Z |
publishDate | 2018-08-01 |
publisher | Instituto de Tecnologia do Paraná (Tecpar) |
record_format | Article |
series | Brazilian Archives of Biology and Technology |
spelling | doaj.art-958531c3aa754059af14917a0059f2b22022-12-21T17:32:58ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242018-08-0161010.1590/1678-4324-2018160406S1516-89132018000100407Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite MaterialsVictor Vasilevich RevinElena Gennadievna KostinaNadezhda Victorovna RevinaVitalina Victorovna ShutovaABSTRACT Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the maximal growth was found in the medium with 4% glucose. It was found that for the alginate production the optimal nitrogen contents could take from 0.05% yeast extract (carbon: nitrogen ratio 168:1). For the first time we demonstrated possibility the A. vinelandii growth during the cultivation in a medium with molasses (a by-product of sugar production) and the significant polysaccharide production (16.6 g/l) was obtained. It was established, that A. vinelandii culture broth could be used as a biological binder for obtaining the biocomposite materials.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100407&lng=en&tlng=enAzotobacter vinelandiialginatenutrient mediumcarbon: nitrogen ratiobiocomposite |
spellingShingle | Victor Vasilevich Revin Elena Gennadievna Kostina Nadezhda Victorovna Revina Vitalina Victorovna Shutova Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials Brazilian Archives of Biology and Technology Azotobacter vinelandii alginate nutrient medium carbon: nitrogen ratio biocomposite |
title | Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials |
title_full | Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials |
title_fullStr | Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials |
title_full_unstemmed | Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials |
title_short | Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials |
title_sort | effect of nutrient sources on the alginate accumulation in the culture liquid of azotobacter vinelandii d 05 and obtaining biocomposite materials |
topic | Azotobacter vinelandii alginate nutrient medium carbon: nitrogen ratio biocomposite |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100407&lng=en&tlng=en |
work_keys_str_mv | AT victorvasilevichrevin effectofnutrientsourcesonthealginateaccumulationinthecultureliquidofazotobactervinelandiid05andobtainingbiocompositematerials AT elenagennadievnakostina effectofnutrientsourcesonthealginateaccumulationinthecultureliquidofazotobactervinelandiid05andobtainingbiocompositematerials AT nadezhdavictorovnarevina effectofnutrientsourcesonthealginateaccumulationinthecultureliquidofazotobactervinelandiid05andobtainingbiocompositematerials AT vitalinavictorovnashutova effectofnutrientsourcesonthealginateaccumulationinthecultureliquidofazotobactervinelandiid05andobtainingbiocompositematerials |