Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering
The development of scaffolding obtained by electrospinning is widely used in tissue engineering due to porous and fibrous structures that can mimic the extracellular matrix. In this study, poly (lactic-co-glycolic acid) (PLGA)/collagen fibers were fabricated by electrospinning method and then evalua...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/5/1079 |
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| author | Aldo Guzmán-Soria Viviana Moreno-Serna Daniel A. Canales Claudio García-Herrera Paula A. Zapata Pedro A. Orihuela |
| author_facet | Aldo Guzmán-Soria Viviana Moreno-Serna Daniel A. Canales Claudio García-Herrera Paula A. Zapata Pedro A. Orihuela |
| author_sort | Aldo Guzmán-Soria |
| collection | DOAJ |
| description | The development of scaffolding obtained by electrospinning is widely used in tissue engineering due to porous and fibrous structures that can mimic the extracellular matrix. In this study, poly (lactic-co-glycolic acid) (PLGA)/collagen fibers were fabricated by electrospinning method and then evaluated in the cell adhesion and viability of human cervical carcinoma HeLa and NIH-3T3 fibroblast for potential application in tissue regeneration. Additionally, collagen release was assessed in NIH-3T3 fibroblasts. The fibrillar morphology of PLGA/collagen fibers was verified by scanning electron microscopy. The fiber diameter decreased in the fibers (PLGA/collagen) up to 0.6 µm. FT-IR spectroscopy and thermal analysis confirmed that both the electrospinning process and the blend with PLGA give structural stability to collagen. Incorporating collagen in the PLGA matrix promotes an increase in the material’s rigidity, showing an increase in the elastic modulus (38%) and tensile strength (70%) compared to pure PLGA. PLGA and PLGA/collagen fibers were found to provide a suitable environment for the adhesion and growth of HeLa and NIH-3T3 cell lines as well as stimulate collagen release. We conclude that these scaffolds could be very effective as biocompatible materials for extracellular matrix regeneration, suggesting their potential applications in tissue bioengineering. |
| first_indexed | 2024-03-11T07:13:46Z |
| format | Article |
| id | doaj.art-95889b1a10314cd3b2777ea076c02adb |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T07:13:46Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-95889b1a10314cd3b2777ea076c02adb2023-11-17T08:25:52ZengMDPI AGPolymers2073-43602023-02-01155107910.3390/polym15051079Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue EngineeringAldo Guzmán-Soria0Viviana Moreno-Serna1Daniel A. Canales2Claudio García-Herrera3Paula A. Zapata4Pedro A. Orihuela5Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Santiago 8320000, ChileQuímica y Farmacia, Facultad de Ciencias de la Salud, Universidad Arturo Prat, Casilla 121, Iquique 1100000, ChileDepartamento de Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Santiago de Chile, USACH, Santiago 8320000, ChileDepartamento de Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Santiago de Chile, USACH, Santiago 8320000, ChileGrupo Polímeros, Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Santiago 8320000, ChileLaboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Santiago 8320000, ChileThe development of scaffolding obtained by electrospinning is widely used in tissue engineering due to porous and fibrous structures that can mimic the extracellular matrix. In this study, poly (lactic-co-glycolic acid) (PLGA)/collagen fibers were fabricated by electrospinning method and then evaluated in the cell adhesion and viability of human cervical carcinoma HeLa and NIH-3T3 fibroblast for potential application in tissue regeneration. Additionally, collagen release was assessed in NIH-3T3 fibroblasts. The fibrillar morphology of PLGA/collagen fibers was verified by scanning electron microscopy. The fiber diameter decreased in the fibers (PLGA/collagen) up to 0.6 µm. FT-IR spectroscopy and thermal analysis confirmed that both the electrospinning process and the blend with PLGA give structural stability to collagen. Incorporating collagen in the PLGA matrix promotes an increase in the material’s rigidity, showing an increase in the elastic modulus (38%) and tensile strength (70%) compared to pure PLGA. PLGA and PLGA/collagen fibers were found to provide a suitable environment for the adhesion and growth of HeLa and NIH-3T3 cell lines as well as stimulate collagen release. We conclude that these scaffolds could be very effective as biocompatible materials for extracellular matrix regeneration, suggesting their potential applications in tissue bioengineering.https://www.mdpi.com/2073-4360/15/5/1079PLGAcollagenscaffoldselectrospun fibersHeLa cellsNIH-3T3 fibroblasts |
| spellingShingle | Aldo Guzmán-Soria Viviana Moreno-Serna Daniel A. Canales Claudio García-Herrera Paula A. Zapata Pedro A. Orihuela Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering Polymers PLGA collagen scaffolds electrospun fibers HeLa cells NIH-3T3 fibroblasts |
| title | Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering |
| title_full | Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering |
| title_fullStr | Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering |
| title_full_unstemmed | Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering |
| title_short | Effect of Electrospun PLGA/Collagen Scaffolds on Cell Adhesion, Viability, and Collagen Release: Potential Applications in Tissue Engineering |
| title_sort | effect of electrospun plga collagen scaffolds on cell adhesion viability and collagen release potential applications in tissue engineering |
| topic | PLGA collagen scaffolds electrospun fibers HeLa cells NIH-3T3 fibroblasts |
| url | https://www.mdpi.com/2073-4360/15/5/1079 |
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