Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly
The Endosomal Sorting Complexes Required for Transport III (ESCRT-III) proteins are critical for cellular membrane scission processes with topologies inverted relative to clathrin-mediated endocytosis. Some viruses appropriate ESCRT-IIIs for their release. By imaging single assembling viral-like par...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/36221 |
| _version_ | 1811199822528512000 |
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| author | Daniel S Johnson Marina Bleck Sanford M Simon |
| author_facet | Daniel S Johnson Marina Bleck Sanford M Simon |
| author_sort | Daniel S Johnson |
| collection | DOAJ |
| description | The Endosomal Sorting Complexes Required for Transport III (ESCRT-III) proteins are critical for cellular membrane scission processes with topologies inverted relative to clathrin-mediated endocytosis. Some viruses appropriate ESCRT-IIIs for their release. By imaging single assembling viral-like particles of HIV-1, we observed that ESCRT-IIIs and the ATPase VPS4 arrive after most of the virion membrane is bent, linger for tens of seconds, and depart ~20 s before scission. These observations suggest that ESCRT-IIIs are recruited by a combination of membrane curvature and the late domains of the HIV-1 Gag protein. ESCRT-IIIs may pull the neck into a narrower form but must leave to allow scission. If scission does not occur within minutes of ESCRT departure, ESCRT-IIIs and VPS4 are recruited again. This mechanistic insight is likely relevant for other ESCRT-dependent scission processes including cell division, endosome tubulation, multivesicular body and nuclear envelope formation, and secretion of exosomes and ectosomes. |
| first_indexed | 2024-04-12T01:53:56Z |
| format | Article |
| id | doaj.art-9588bebac7b74085adec9bccc738abac |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:53:56Z |
| publishDate | 2018-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9588bebac7b74085adec9bccc738abac2022-12-22T03:52:51ZengeLife Sciences Publications LtdeLife2050-084X2018-07-01710.7554/eLife.36221Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assemblyDaniel S Johnson0https://orcid.org/0000-0001-8906-0509Marina Bleck1https://orcid.org/0000-0003-4175-8363Sanford M Simon2https://orcid.org/0000-0002-8615-4224Laboratory of Cellular Biophysics, The Rockefeller University, New York, United States; Department of Physics and Astronomy, Hofstra University, Hempstead, United StatesLaboratory of Cellular Biophysics, The Rockefeller University, New York, United StatesLaboratory of Cellular Biophysics, The Rockefeller University, New York, United StatesThe Endosomal Sorting Complexes Required for Transport III (ESCRT-III) proteins are critical for cellular membrane scission processes with topologies inverted relative to clathrin-mediated endocytosis. Some viruses appropriate ESCRT-IIIs for their release. By imaging single assembling viral-like particles of HIV-1, we observed that ESCRT-IIIs and the ATPase VPS4 arrive after most of the virion membrane is bent, linger for tens of seconds, and depart ~20 s before scission. These observations suggest that ESCRT-IIIs are recruited by a combination of membrane curvature and the late domains of the HIV-1 Gag protein. ESCRT-IIIs may pull the neck into a narrower form but must leave to allow scission. If scission does not occur within minutes of ESCRT departure, ESCRT-IIIs and VPS4 are recruited again. This mechanistic insight is likely relevant for other ESCRT-dependent scission processes including cell division, endosome tubulation, multivesicular body and nuclear envelope formation, and secretion of exosomes and ectosomes.https://elifesciences.org/articles/36221HIV-1ESCRTviral assemblymembrane scission |
| spellingShingle | Daniel S Johnson Marina Bleck Sanford M Simon Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly eLife HIV-1 ESCRT viral assembly membrane scission |
| title | Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly |
| title_full | Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly |
| title_fullStr | Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly |
| title_full_unstemmed | Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly |
| title_short | Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly |
| title_sort | timing of escrt iii protein recruitment and membrane scission during hiv 1 assembly |
| topic | HIV-1 ESCRT viral assembly membrane scission |
| url | https://elifesciences.org/articles/36221 |
| work_keys_str_mv | AT danielsjohnson timingofescrtiiiproteinrecruitmentandmembranescissionduringhiv1assembly AT marinableck timingofescrtiiiproteinrecruitmentandmembranescissionduringhiv1assembly AT sanfordmsimon timingofescrtiiiproteinrecruitmentandmembranescissionduringhiv1assembly |