Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i>
Global warming and drought stress are expected to have a negative impact on agricultural productivity. Desiccation-tolerant species, which are able to tolerate the almost complete desiccation of their vegetative tissues, are appropriate models to study extreme drought tolerance and identify novel ap...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8520 |
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| author | Petko Mladenov Diana Zasheva Sébastien Planchon Céline C. Leclercq Denis Falconet Lucas Moyet Sabine Brugière Daniela Moyankova Magdalena Tchorbadjieva Myriam Ferro Norbert Rolland Jenny Renaut Dimitar Djilianov Xin Deng |
| author_facet | Petko Mladenov Diana Zasheva Sébastien Planchon Céline C. Leclercq Denis Falconet Lucas Moyet Sabine Brugière Daniela Moyankova Magdalena Tchorbadjieva Myriam Ferro Norbert Rolland Jenny Renaut Dimitar Djilianov Xin Deng |
| author_sort | Petko Mladenov |
| collection | DOAJ |
| description | Global warming and drought stress are expected to have a negative impact on agricultural productivity. Desiccation-tolerant species, which are able to tolerate the almost complete desiccation of their vegetative tissues, are appropriate models to study extreme drought tolerance and identify novel approaches to improve the resistance of crops to drought stress. In the present study, to better understand what makes resurrection plants extremely tolerant to drought, we performed transmission electron microscopy and integrative large-scale proteomics, including organellar and phosphorylation proteomics, and combined these investigations with previously published transcriptomic and metabolomics data from the resurrection plant <i>Haberlea rhodopensis</i>. The results revealed new evidence about organelle and cell preservation, posttranscriptional and posttranslational regulation, photosynthesis, primary metabolism, autophagy, and cell death in response to desiccation in <i>H. rhodopensis.</i> Different protective intrinsically disordered proteins, such as late embryogenesis abundant (LEA) proteins, thaumatin-like proteins (TLPs), and heat shock proteins (HSPs), were detected. We also found a constitutively abundant dehydrin in <i>H. rhodopensis</i> whose phosphorylation levels increased under stress in the chloroplast fraction. This integrative multi-omics analysis revealed a systemic response to desiccation in <i>H. rhodopensis</i> and certain targets for further genomic and evolutionary studies on DT mechanisms and genetic engineering towards the improvement of drought tolerance in crops. |
| first_indexed | 2024-03-09T10:08:43Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:08:43Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-958d3fbcf43247e6977bc888bc172cb92023-12-01T22:57:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315852010.3390/ijms23158520Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i>Petko Mladenov0Diana Zasheva1Sébastien Planchon2Céline C. Leclercq3Denis Falconet4Lucas Moyet5Sabine Brugière6Daniela Moyankova7Magdalena Tchorbadjieva8Myriam Ferro9Norbert Rolland10Jenny Renaut11Dimitar Djilianov12Xin Deng13Agrobioinstitute, Agricultural Academy Bulgaria, 1164 Sofia, BulgariaInstitute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaEnvironmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, L-4362 Esch-sur-Alzette, LuxembourgEnvironmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, L-4362 Esch-sur-Alzette, LuxembourgLaboratoire de Physiologie Cellulaire et Végétale, University Grenoble Alpes, CNRS, INRAE, CEA, 38054 Grenoble, FranceLaboratoire de Physiologie Cellulaire et Végétale, University Grenoble Alpes, CNRS, INRAE, CEA, 38054 Grenoble, FranceLaboratoire de Biologie à Grande Echelle, Institut de Recherches en Technologies et Sciences pour le Vivant, CEA, Université Grenoble Alpes INSERM, 38054 Grenoble, FranceAgrobioinstitute, Agricultural Academy Bulgaria, 1164 Sofia, BulgariaDepartment of Biochemistry, Faculty of Biology, Sofia University, 1164 Sofia, BulgariaLaboratoire de Biologie à Grande Echelle, Institut de Recherches en Technologies et Sciences pour le Vivant, CEA, Université Grenoble Alpes INSERM, 38054 Grenoble, FranceLaboratoire de Physiologie Cellulaire et Végétale, University Grenoble Alpes, CNRS, INRAE, CEA, 38054 Grenoble, FranceEnvironmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, L-4362 Esch-sur-Alzette, LuxembourgAgrobioinstitute, Agricultural Academy Bulgaria, 1164 Sofia, BulgariaKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, ChinaGlobal warming and drought stress are expected to have a negative impact on agricultural productivity. Desiccation-tolerant species, which are able to tolerate the almost complete desiccation of their vegetative tissues, are appropriate models to study extreme drought tolerance and identify novel approaches to improve the resistance of crops to drought stress. In the present study, to better understand what makes resurrection plants extremely tolerant to drought, we performed transmission electron microscopy and integrative large-scale proteomics, including organellar and phosphorylation proteomics, and combined these investigations with previously published transcriptomic and metabolomics data from the resurrection plant <i>Haberlea rhodopensis</i>. The results revealed new evidence about organelle and cell preservation, posttranscriptional and posttranslational regulation, photosynthesis, primary metabolism, autophagy, and cell death in response to desiccation in <i>H. rhodopensis.</i> Different protective intrinsically disordered proteins, such as late embryogenesis abundant (LEA) proteins, thaumatin-like proteins (TLPs), and heat shock proteins (HSPs), were detected. We also found a constitutively abundant dehydrin in <i>H. rhodopensis</i> whose phosphorylation levels increased under stress in the chloroplast fraction. This integrative multi-omics analysis revealed a systemic response to desiccation in <i>H. rhodopensis</i> and certain targets for further genomic and evolutionary studies on DT mechanisms and genetic engineering towards the improvement of drought tolerance in crops.https://www.mdpi.com/1422-0067/23/15/8520resurrection plantproteomicssystems biologysubcellular fractionationdrought stressdehydrin |
| spellingShingle | Petko Mladenov Diana Zasheva Sébastien Planchon Céline C. Leclercq Denis Falconet Lucas Moyet Sabine Brugière Daniela Moyankova Magdalena Tchorbadjieva Myriam Ferro Norbert Rolland Jenny Renaut Dimitar Djilianov Xin Deng Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i> International Journal of Molecular Sciences resurrection plant proteomics systems biology subcellular fractionation drought stress dehydrin |
| title | Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i> |
| title_full | Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i> |
| title_fullStr | Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i> |
| title_full_unstemmed | Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i> |
| title_short | Proteomics Evidence of a Systemic Response to Desiccation in the Resurrection Plant <i>Haberlea rhodopensis</i> |
| title_sort | proteomics evidence of a systemic response to desiccation in the resurrection plant i haberlea rhodopensis i |
| topic | resurrection plant proteomics systems biology subcellular fractionation drought stress dehydrin |
| url | https://www.mdpi.com/1422-0067/23/15/8520 |
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