Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus
Background: Infection with Echinococcus granulosus causes hydatidosis in human and ruminants. With regards to the high prevalence of hydatidosis in Iran, dealing with this disease is important in terms of public health. Objective: The aim of this study was to construct pEGFP-ChEgTrp as DNA model fo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
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Qazvin University of Medical Sciences & Health Services
2015-12-01
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| Series: | The Journal of Qazvin University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.qums.ac.ir/browse.php?a_id=1969&slc_lang=en&sid=1&ftxt=1 |
| _version_ | 1828472171871076352 |
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| author | M. Ahmadzadeh M. Esmaelizad SA. Angaji M. Tahmaseb S. Mohammadi R. Mesri |
| author_facet | M. Ahmadzadeh M. Esmaelizad SA. Angaji M. Tahmaseb S. Mohammadi R. Mesri |
| author_sort | M. Ahmadzadeh |
| collection | DOAJ |
| description | Background: Infection with Echinococcus granulosus causes hydatidosis in human and ruminants. With regards to the high prevalence of hydatidosis in Iran, dealing with this disease is important in terms of public health.
Objective: The aim of this study was to construct pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus
Methods: This experimental study was conducted in the Razi Vaccine & Serum Research Institute, Karaj in 2013. Initially, epitopes stimulating the host immune response were predicted by IEDB Database and the coding sequences were made. The sequences were amplified by PCR. The PCR products were cloned into pEGFP-N1 vector after digestion with XhoI restriction enzyme. The bacteria containing recombinant plasmid were evaluated using Colony PCR, agarose gel electrophoresis and sequencing methods.
Findings: Four peptides with 10 linear epitopes were predicted in EgTrp antigen. The nucleotide sequence coding ChEgTrp was amplified by PCR using specific primers and a 270 bp fragment was obtained. This fragment was cloned into pEGFP-N1 vector and the recombinant plasmid was confirmed by Colony PCR and agarose gel electrophoresis. For final confirmation, the recombinant plasmid was sequenced and the pEGFP-ChEgTrp was constructed.
Conclusion: The ChEgTrp was successfully cloned into the pEGFP-N1 vector and this plasmid can be used to design DNA vaccines. |
| first_indexed | 2024-12-11T05:25:42Z |
| format | Article |
| id | doaj.art-9591dabb62894ad4bf91a3d09c733228 |
| institution | Directory Open Access Journal |
| issn | 1561-3666 2228-7213 |
| language | fas |
| last_indexed | 2024-12-11T05:25:42Z |
| publishDate | 2015-12-01 |
| publisher | Qazvin University of Medical Sciences & Health Services |
| record_format | Article |
| series | The Journal of Qazvin University of Medical Sciences |
| spelling | doaj.art-9591dabb62894ad4bf91a3d09c7332282022-12-22T01:19:35ZfasQazvin University of Medical Sciences & Health ServicesThe Journal of Qazvin University of Medical Sciences1561-36662228-72132015-12-01194411Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosusM. AhmadzadehM. EsmaelizadSA. AngajiM. TahmasebS. MohammadiR. MesriBackground: Infection with Echinococcus granulosus causes hydatidosis in human and ruminants. With regards to the high prevalence of hydatidosis in Iran, dealing with this disease is important in terms of public health. Objective: The aim of this study was to construct pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus Methods: This experimental study was conducted in the Razi Vaccine & Serum Research Institute, Karaj in 2013. Initially, epitopes stimulating the host immune response were predicted by IEDB Database and the coding sequences were made. The sequences were amplified by PCR. The PCR products were cloned into pEGFP-N1 vector after digestion with XhoI restriction enzyme. The bacteria containing recombinant plasmid were evaluated using Colony PCR, agarose gel electrophoresis and sequencing methods. Findings: Four peptides with 10 linear epitopes were predicted in EgTrp antigen. The nucleotide sequence coding ChEgTrp was amplified by PCR using specific primers and a 270 bp fragment was obtained. This fragment was cloned into pEGFP-N1 vector and the recombinant plasmid was confirmed by Colony PCR and agarose gel electrophoresis. For final confirmation, the recombinant plasmid was sequenced and the pEGFP-ChEgTrp was constructed. Conclusion: The ChEgTrp was successfully cloned into the pEGFP-N1 vector and this plasmid can be used to design DNA vaccines.http://journal.qums.ac.ir/browse.php?a_id=1969&slc_lang=en&sid=1&ftxt=1EchinococcosisTropomyosinMolecular CloningDNA Vaccines |
| spellingShingle | M. Ahmadzadeh M. Esmaelizad SA. Angaji M. Tahmaseb S. Mohammadi R. Mesri Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus The Journal of Qazvin University of Medical Sciences Echinococcosis Tropomyosin Molecular Cloning DNA Vaccines |
| title | Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus |
| title_full | Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus |
| title_fullStr | Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus |
| title_full_unstemmed | Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus |
| title_short | Construction of pEGFP-ChEgTrp as DNA model for multi-epitope vaccine against Echinococcus granulosus |
| title_sort | construction of pegfp chegtrp as dna model for multi epitope vaccine against echinococcus granulosus |
| topic | Echinococcosis Tropomyosin Molecular Cloning DNA Vaccines |
| url | http://journal.qums.ac.ir/browse.php?a_id=1969&slc_lang=en&sid=1&ftxt=1 |
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