In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity
Abstract Background The current study investigates the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate. Results Aerial parts extract exhibited higher DPPH scavenging activi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2021-08-01
|
| Series: | Future Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43094-021-00333-0 |
| _version_ | 1831763503931719680 |
|---|---|
| author | Ammar Mahmoud Ibrahim Mariani Abdul Hamid Rama Ali Althiab Amir Husni Mohd Shariff Razauden Mohamed Zulkifli |
| author_facet | Ammar Mahmoud Ibrahim Mariani Abdul Hamid Rama Ali Althiab Amir Husni Mohd Shariff Razauden Mohamed Zulkifli |
| author_sort | Ammar Mahmoud Ibrahim |
| collection | DOAJ |
| description | Abstract Background The current study investigates the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate. Results Aerial parts extract exhibited higher DPPH scavenging activity compared to root extract with IC50 of 62 µg/mL and 206 µg/mL, respectively. Both aerial parts and root extracts showed low cytotoxicity towards fibroblasts with 753 µg/mL LD50 for aerial parts and undetected LD50 for root extract. Additionally, aerial parts extract significantly induces fibroblasts proliferation up to 134%. Wound closure investigation showed a significant closure percentage for aerial parts compared to untreated control with 75% at 1 µg/mL and high closure percentage with 70% at 0.1 µg/mL for root extract compared to only 59% closure percentage for untreated control after 48 h of the study. Conclusions This study provided evidence for A. indica to have great wound healing potential. The finding builds the scientific background in future to utilise the high antioxidant activity of A. indica and its ability to stimulate fibroblasts migration and proliferation for further applications. |
| first_indexed | 2024-12-22T05:26:26Z |
| format | Article |
| id | doaj.art-95949251d3bc40a48f2bc7bdbd57052d |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-12-22T05:26:26Z |
| publishDate | 2021-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-95949251d3bc40a48f2bc7bdbd57052d2022-12-21T18:37:34ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532021-08-01711610.1186/s43094-021-00333-0In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activityAmmar Mahmoud Ibrahim0Mariani Abdul Hamid1Rama Ali Althiab2Amir Husni Mohd Shariff3Razauden Mohamed Zulkifli4Department of Biosciences, Faculty of Science, Universiti Teknologi MalaysiaSchool of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi MalaysiaDepartment of Biosciences, Faculty of Science, Universiti Teknologi MalaysiaDepartment of Biosciences, Faculty of Science, Universiti Teknologi MalaysiaDepartment of Biosciences, Faculty of Science, Universiti Teknologi MalaysiaAbstract Background The current study investigates the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate. Results Aerial parts extract exhibited higher DPPH scavenging activity compared to root extract with IC50 of 62 µg/mL and 206 µg/mL, respectively. Both aerial parts and root extracts showed low cytotoxicity towards fibroblasts with 753 µg/mL LD50 for aerial parts and undetected LD50 for root extract. Additionally, aerial parts extract significantly induces fibroblasts proliferation up to 134%. Wound closure investigation showed a significant closure percentage for aerial parts compared to untreated control with 75% at 1 µg/mL and high closure percentage with 70% at 0.1 µg/mL for root extract compared to only 59% closure percentage for untreated control after 48 h of the study. Conclusions This study provided evidence for A. indica to have great wound healing potential. The finding builds the scientific background in future to utilise the high antioxidant activity of A. indica and its ability to stimulate fibroblasts migration and proliferation for further applications.https://doi.org/10.1186/s43094-021-00333-0In vitroAcalypha indicaWound healingCytotoxicityAntioxidant |
| spellingShingle | Ammar Mahmoud Ibrahim Mariani Abdul Hamid Rama Ali Althiab Amir Husni Mohd Shariff Razauden Mohamed Zulkifli In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity Future Journal of Pharmaceutical Sciences In vitro Acalypha indica Wound healing Cytotoxicity Antioxidant |
| title | In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity |
| title_full | In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity |
| title_fullStr | In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity |
| title_full_unstemmed | In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity |
| title_short | In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity |
| title_sort | in vitro fibroblasts viability and migration stimulation of acalypha indica an insight on wound healing activity |
| topic | In vitro Acalypha indica Wound healing Cytotoxicity Antioxidant |
| url | https://doi.org/10.1186/s43094-021-00333-0 |
| work_keys_str_mv | AT ammarmahmoudibrahim invitrofibroblastsviabilityandmigrationstimulationofacalyphaindicaaninsightonwoundhealingactivity AT marianiabdulhamid invitrofibroblastsviabilityandmigrationstimulationofacalyphaindicaaninsightonwoundhealingactivity AT ramaalialthiab invitrofibroblastsviabilityandmigrationstimulationofacalyphaindicaaninsightonwoundhealingactivity AT amirhusnimohdshariff invitrofibroblastsviabilityandmigrationstimulationofacalyphaindicaaninsightonwoundhealingactivity AT razaudenmohamedzulkifli invitrofibroblastsviabilityandmigrationstimulationofacalyphaindicaaninsightonwoundhealingactivity |