Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability
As the main component of <i>Platycladus orientalis</i>, cedrol has known germinal activity. A range of cedrol formulations have been developed to prevent hair-loss, but compliance remains key issues. In this study, we prepared cedrol nanoemulsion (CE-NE) and determined the particle size...
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/6/1795 |
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| author | Yaling Deng Feixue Huang Jiewen Wang Yumeng Zhang Yan Zhang Guangyue Su Yuqing Zhao |
| author_facet | Yaling Deng Feixue Huang Jiewen Wang Yumeng Zhang Yan Zhang Guangyue Su Yuqing Zhao |
| author_sort | Yaling Deng |
| collection | DOAJ |
| description | As the main component of <i>Platycladus orientalis</i>, cedrol has known germinal activity. A range of cedrol formulations have been developed to prevent hair-loss, but compliance remains key issues. In this study, we prepared cedrol nanoemulsion (CE-NE) and determined the particle size and PDI (polydispersion coefficient), investigated the hair growth activity and studied the bioavailability in vitro and in vivo. Results showed that the average particle size of CE-NE is 14.26 ± 0.16 nm, and the PDI value is 0.086 ± 0.019. In vitro drug release investigation and drug release kinetics analysis showed release profile of CE from nanoparticles demonstrates the preferred partition of CE in buffer pH 4.0, the release profile of CE-NE showed a first-order kinetics reaching around 36.7% after 6 h at 37 °C. We artificially depilated the back hair of C57BL/6 mice and compared the efficacy of a designed cedrol nanoemulsion to an existing ointment group. The hair follicles were imaged and quantified using a digital photomicrograph. The results showed that compared with the ointment, CE-NE had positive effects on hair growth, improved drug solubility. Compared with the ointment and 2% minoxidil groups, 50 mg/mL CE-NE led to more robust hair growth. Pharmacokinetics analysis showed that the AUC<sub>0–<i>t</i></sub> of CE-NE was 4-fold higher than that of the ointment group, confirming that the bioavailability of the nanoemulsion was greater than that of the ointment. CE-NE also significantly reduced the hair growth time of model mice and significantly increased the growth rate of hair follicles. In conclusion, these data suggest that the nanoemulsion significantly improved the pharmacokinetic properties and hair growth effects cedrol, enhancing its efficacy in vitro and in vivo. |
| first_indexed | 2024-03-10T13:00:09Z |
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| language | English |
| last_indexed | 2024-03-10T13:00:09Z |
| publishDate | 2021-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9597b37c7b094e56865692f0b27c8df12023-11-21T11:36:43ZengMDPI AGMolecules1420-30492021-03-01266179510.3390/molecules26061795Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its BioavailabilityYaling Deng0Feixue Huang1Jiewen Wang2Yumeng Zhang3Yan Zhang4Guangyue Su5Yuqing Zhao6Traditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTraditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTraditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTraditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTraditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTraditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaTraditional Chinese Medicine College, Shenyang Pharmaceutical University, Shenyang 110016, ChinaAs the main component of <i>Platycladus orientalis</i>, cedrol has known germinal activity. A range of cedrol formulations have been developed to prevent hair-loss, but compliance remains key issues. In this study, we prepared cedrol nanoemulsion (CE-NE) and determined the particle size and PDI (polydispersion coefficient), investigated the hair growth activity and studied the bioavailability in vitro and in vivo. Results showed that the average particle size of CE-NE is 14.26 ± 0.16 nm, and the PDI value is 0.086 ± 0.019. In vitro drug release investigation and drug release kinetics analysis showed release profile of CE from nanoparticles demonstrates the preferred partition of CE in buffer pH 4.0, the release profile of CE-NE showed a first-order kinetics reaching around 36.7% after 6 h at 37 °C. We artificially depilated the back hair of C57BL/6 mice and compared the efficacy of a designed cedrol nanoemulsion to an existing ointment group. The hair follicles were imaged and quantified using a digital photomicrograph. The results showed that compared with the ointment, CE-NE had positive effects on hair growth, improved drug solubility. Compared with the ointment and 2% minoxidil groups, 50 mg/mL CE-NE led to more robust hair growth. Pharmacokinetics analysis showed that the AUC<sub>0–<i>t</i></sub> of CE-NE was 4-fold higher than that of the ointment group, confirming that the bioavailability of the nanoemulsion was greater than that of the ointment. CE-NE also significantly reduced the hair growth time of model mice and significantly increased the growth rate of hair follicles. In conclusion, these data suggest that the nanoemulsion significantly improved the pharmacokinetic properties and hair growth effects cedrol, enhancing its efficacy in vitro and in vivo.https://www.mdpi.com/1420-3049/26/6/1795cedrolnanoemulsionhair growthpharmacokineticspharmacodynamics |
| spellingShingle | Yaling Deng Feixue Huang Jiewen Wang Yumeng Zhang Yan Zhang Guangyue Su Yuqing Zhao Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability Molecules cedrol nanoemulsion hair growth pharmacokinetics pharmacodynamics |
| title | Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability |
| title_full | Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability |
| title_fullStr | Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability |
| title_full_unstemmed | Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability |
| title_short | Hair Growth Promoting Activity of Cedrol Nanoemulsion in C57BL/6 Mice and Its Bioavailability |
| title_sort | hair growth promoting activity of cedrol nanoemulsion in c57bl 6 mice and its bioavailability |
| topic | cedrol nanoemulsion hair growth pharmacokinetics pharmacodynamics |
| url | https://www.mdpi.com/1420-3049/26/6/1795 |
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