Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle
The study was planned with objective to screen single nucleotide polymorphisms (SNPs) in bovine HSPB1 gene and to find its effect on age at calving in Karan-Fries (Bos taurus × Bos indicus) and Sahiwal (Bos indicus) breeds of cattle. Genomic DNA was extracted from whole blood of 180 cows of both br...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2018-05-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/79869 |
| _version_ | 1811169029518262272 |
|---|---|
| author | M V CHAUDHARI I D GUPTA ARCHANA VERMA AVTAR SINGH RAMENDRA DAS L SAILO S V SINGH |
| author_facet | M V CHAUDHARI I D GUPTA ARCHANA VERMA AVTAR SINGH RAMENDRA DAS L SAILO S V SINGH |
| author_sort | M V CHAUDHARI |
| collection | DOAJ |
| description |
The study was planned with objective to screen single nucleotide polymorphisms (SNPs) in bovine HSPB1 gene and to find its effect on age at calving in Karan-Fries (Bos taurus × Bos indicus) and Sahiwal (Bos indicus) breeds of cattle. Genomic DNA was extracted from whole blood of 180 cows of both breeds. Based on publically available bovine HSPB1 gene sequence, one primer set was used for polymerase chain reaction amplification of the target region. Further DNA sequencing revealed a transition of thymine to cytosine at SNP rs208395876 in 5-UTR and a silent transversion of guanine to thymine at SNP rs723061520 in first coding sequence of bovine HSPB1 gene in both the studied breeds. Effect of individual SNP genotypes of bovine HSPB1 gene with age at calving (months) was analyzed separately in both breeds via regression using a repeated gene substitution MIXED model and least-squares means.The overall observed heterozygosity in both breeds and the F-Statistics values indicated that there was lesser genetic diversity in studied genomic region of bovine HSPB1 gene in Bos indicus compared to crossbred cattle. Association analysis revealed that SNP rs208395876 significantly delayed age at calving in Karan-Fries cows. In conclusion, the studied genomic region of bovine HSPB1 gene is polymorphic. In addition, these polymorphisms were informative with regard to age at calving of crossbred cows. Therefore, this gene is an important candidate for cow fertility.
|
| first_indexed | 2024-04-10T16:35:50Z |
| format | Article |
| id | doaj.art-9598114ba6c74866bec33e3ce06010e8 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:35:50Z |
| publishDate | 2018-05-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-9598114ba6c74866bec33e3ce06010e82023-02-08T11:41:28ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272018-05-01871210.56093/ijans.v87i12.79869Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattleM V CHAUDHARI0I D GUPTA1ARCHANA VERMA2AVTAR SINGH3RAMENDRA DAS4L SAILO5S V SINGH6ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India The study was planned with objective to screen single nucleotide polymorphisms (SNPs) in bovine HSPB1 gene and to find its effect on age at calving in Karan-Fries (Bos taurus × Bos indicus) and Sahiwal (Bos indicus) breeds of cattle. Genomic DNA was extracted from whole blood of 180 cows of both breeds. Based on publically available bovine HSPB1 gene sequence, one primer set was used for polymerase chain reaction amplification of the target region. Further DNA sequencing revealed a transition of thymine to cytosine at SNP rs208395876 in 5-UTR and a silent transversion of guanine to thymine at SNP rs723061520 in first coding sequence of bovine HSPB1 gene in both the studied breeds. Effect of individual SNP genotypes of bovine HSPB1 gene with age at calving (months) was analyzed separately in both breeds via regression using a repeated gene substitution MIXED model and least-squares means.The overall observed heterozygosity in both breeds and the F-Statistics values indicated that there was lesser genetic diversity in studied genomic region of bovine HSPB1 gene in Bos indicus compared to crossbred cattle. Association analysis revealed that SNP rs208395876 significantly delayed age at calving in Karan-Fries cows. In conclusion, the studied genomic region of bovine HSPB1 gene is polymorphic. In addition, these polymorphisms were informative with regard to age at calving of crossbred cows. Therefore, this gene is an important candidate for cow fertility. https://epubs.icar.org.in/index.php/IJAnS/article/view/79869Age at calvingBos indicusGene substitution modelHSPB1 geneSNP |
| spellingShingle | M V CHAUDHARI I D GUPTA ARCHANA VERMA AVTAR SINGH RAMENDRA DAS L SAILO S V SINGH Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle Indian Journal of Animal Sciences Age at calving Bos indicus Gene substitution model HSPB1 gene SNP |
| title | Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle |
| title_full | Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle |
| title_fullStr | Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle |
| title_full_unstemmed | Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle |
| title_short | Gene substitution effect of bovine heat shock protein beta-1 gene polymorphism on age at calving in Indian dairy cattle |
| title_sort | gene substitution effect of bovine heat shock protein beta 1 gene polymorphism on age at calving in indian dairy cattle |
| topic | Age at calving Bos indicus Gene substitution model HSPB1 gene SNP |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/79869 |
| work_keys_str_mv | AT mvchaudhari genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle AT idgupta genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle AT archanaverma genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle AT avtarsingh genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle AT ramendradas genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle AT lsailo genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle AT svsingh genesubstitutioneffectofbovineheatshockproteinbeta1genepolymorphismonageatcalvinginindiandairycattle |