Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms
Immuno-modulatory effects of seventeen lactic acid bacteria (LAB) and probiotic strains were assessed following stimulation with peripheral blood mononuclear cells. Pro- and anti-inflammatory cytokines including interleukin (IL)-2, IL-4, IL-10, IL-12 p70, interferon (IFN)-γ, tumor necrosis factor (T...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464613002600 |
| _version_ | 1819293746224693248 |
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| author | R. Ashraf T. Vasiljevic S.L. Day S.C. Smith O.N. Donkor |
| author_facet | R. Ashraf T. Vasiljevic S.L. Day S.C. Smith O.N. Donkor |
| author_sort | R. Ashraf |
| collection | DOAJ |
| description | Immuno-modulatory effects of seventeen lactic acid bacteria (LAB) and probiotic strains were assessed following stimulation with peripheral blood mononuclear cells. Pro- and anti-inflammatory cytokines including interleukin (IL)-2, IL-4, IL-10, IL-12 p70, interferon (IFN)-γ, tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β measured at different time points shows that all strains stimulated significant cytokines in varying concentrations and strain specific manner. Overall, TNF-α, IFN-γ, IL-10 and TGF-β were stimulated at high concentrations where Lactobacillus casei 290 and Streptococcus thermophilus M5 stimulated TNF-α and IFN-γ and Bifidobacterium strains stimulated maximum levels of IL-10 and TGF-β respectively. A significant increase in CD3+CD4+CD25+ and Treg population was observed where S. thermophilus M5 and 1342, B. animalis subsp. lactis BB12 and L. casei 290 showed seven-fold increase in FoxP3 expression. The study suggests that LAB and probiotics strains differ in their immuno-modulatory responses and functions, which necessitates their careful selection and characterization before applications. |
| first_indexed | 2024-12-24T04:15:19Z |
| format | Article |
| id | doaj.art-95994b3934824b8c9dca8c323edc7532 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:15:19Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-95994b3934824b8c9dca8c323edc75322022-12-21T17:15:57ZengElsevierJournal of Functional Foods1756-46462014-01-016395409Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanismsR. Ashraf0T. Vasiljevic1S.L. Day2S.C. Smith3O.N. Donkor4Advanced Food Systems Research Unit, College of Health Science and Biomedicine, Victoria University, Werribee Campus, P.O. Box 14428, Melbourne, Vic 8001, AustraliaAdvanced Food Systems Research Unit, College of Health Science and Biomedicine, Victoria University, Werribee Campus, P.O. Box 14428, Melbourne, Vic 8001, AustraliaBurnet Institute, Centre for Immunology, Melbourne, Vic 3004, AustraliaSchool of Exercise and Nutrition Sciences, Deakin University, Vic 3125, AustraliaAdvanced Food Systems Research Unit, College of Health Science and Biomedicine, Victoria University, Werribee Campus, P.O. Box 14428, Melbourne, Vic 8001, Australia; Corresponding author. Tel.: +61 3 9919 8059; fax: +61 3 9919 8284.Immuno-modulatory effects of seventeen lactic acid bacteria (LAB) and probiotic strains were assessed following stimulation with peripheral blood mononuclear cells. Pro- and anti-inflammatory cytokines including interleukin (IL)-2, IL-4, IL-10, IL-12 p70, interferon (IFN)-γ, tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β measured at different time points shows that all strains stimulated significant cytokines in varying concentrations and strain specific manner. Overall, TNF-α, IFN-γ, IL-10 and TGF-β were stimulated at high concentrations where Lactobacillus casei 290 and Streptococcus thermophilus M5 stimulated TNF-α and IFN-γ and Bifidobacterium strains stimulated maximum levels of IL-10 and TGF-β respectively. A significant increase in CD3+CD4+CD25+ and Treg population was observed where S. thermophilus M5 and 1342, B. animalis subsp. lactis BB12 and L. casei 290 showed seven-fold increase in FoxP3 expression. The study suggests that LAB and probiotics strains differ in their immuno-modulatory responses and functions, which necessitates their careful selection and characterization before applications.http://www.sciencedirect.com/science/article/pii/S1756464613002600ProbioticsLactic acid bacteriaCytokinesImmuno-modulationCD4+CD25+FoxP3+ regulatory T cells |
| spellingShingle | R. Ashraf T. Vasiljevic S.L. Day S.C. Smith O.N. Donkor Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms Journal of Functional Foods Probiotics Lactic acid bacteria Cytokines Immuno-modulation CD4+CD25+FoxP3+ regulatory T cells |
| title | Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms |
| title_full | Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms |
| title_fullStr | Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms |
| title_full_unstemmed | Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms |
| title_short | Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms |
| title_sort | lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory t cells mechanisms |
| topic | Probiotics Lactic acid bacteria Cytokines Immuno-modulation CD4+CD25+FoxP3+ regulatory T cells |
| url | http://www.sciencedirect.com/science/article/pii/S1756464613002600 |
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