A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity.
Ferritin, an iron homeostasis protein, has important functions in transition and storage of toxic metal ions. In this study, the full-length cDNA of ferritin was isolated from Dendrorhynchus zhejiangensis by cDNA library and RACE approaches. The higher similarity and conserved motifs for ferritin we...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3532109?pdf=render |
| _version_ | 1811264650038214656 |
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| author | Chenghua Li Zhen Li Ye Li Jun Zhou Chundan Zhang Xiurong Su Taiwu Li |
| author_facet | Chenghua Li Zhen Li Ye Li Jun Zhou Chundan Zhang Xiurong Su Taiwu Li |
| author_sort | Chenghua Li |
| collection | DOAJ |
| description | Ferritin, an iron homeostasis protein, has important functions in transition and storage of toxic metal ions. In this study, the full-length cDNA of ferritin was isolated from Dendrorhynchus zhejiangensis by cDNA library and RACE approaches. The higher similarity and conserved motifs for ferritin were also identified in worm counterparts, indicating that it belonged to a new member of ferritin family. The temporal expression of worm ferritin in haemocytes was analyzed by RT-PCR, and revealed the ferritin could be induced by Cd(2+), Pb(2+) and Fe(2+). The heavy metal binding activity of recombinant ferritin was further elucidated by atomic force microscopy (AFM). It was observed that the ferritin protein could form a chain of beads with different size against three metals exposure, and the largest one with 35~40 nm in height was identified in the Cd(2+) challenge group. Our results indicated that worm ferritin was a promising candidate for heavy metals detoxification. |
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| id | doaj.art-959b7b7eb596445eb4e9e3b3badd9b22 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:08:14Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-959b7b7eb596445eb4e9e3b3badd9b222022-12-22T03:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5142810.1371/journal.pone.0051428A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity.Chenghua LiZhen LiYe LiJun ZhouChundan ZhangXiurong SuTaiwu LiFerritin, an iron homeostasis protein, has important functions in transition and storage of toxic metal ions. In this study, the full-length cDNA of ferritin was isolated from Dendrorhynchus zhejiangensis by cDNA library and RACE approaches. The higher similarity and conserved motifs for ferritin were also identified in worm counterparts, indicating that it belonged to a new member of ferritin family. The temporal expression of worm ferritin in haemocytes was analyzed by RT-PCR, and revealed the ferritin could be induced by Cd(2+), Pb(2+) and Fe(2+). The heavy metal binding activity of recombinant ferritin was further elucidated by atomic force microscopy (AFM). It was observed that the ferritin protein could form a chain of beads with different size against three metals exposure, and the largest one with 35~40 nm in height was identified in the Cd(2+) challenge group. Our results indicated that worm ferritin was a promising candidate for heavy metals detoxification.http://europepmc.org/articles/PMC3532109?pdf=render |
| spellingShingle | Chenghua Li Zhen Li Ye Li Jun Zhou Chundan Zhang Xiurong Su Taiwu Li A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity. PLoS ONE |
| title | A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity. |
| title_full | A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity. |
| title_fullStr | A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity. |
| title_full_unstemmed | A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity. |
| title_short | A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity. |
| title_sort | ferritin from dendrorhynchus zhejiangensis with heavy metals detoxification activity |
| url | http://europepmc.org/articles/PMC3532109?pdf=render |
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