Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices
Conventional manufacturing methods for polydimethylsiloxane (PDMS)-based microdevices require multiple steps and elements that increase cost and production time. Also, these PDMS microdevices are mostly limited to single use, and it is difficult to recover the contents inside the microchannels or pe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/13/5/650 |
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| author | Camilo Pérez-Sosa Ana Belén Peñaherrera-Pazmiño Gustavo Rosero Natalia Bourguignon Aparna Aravelli Shekhar Bhansali Maximiliano Sebastian Pérez Betiana Lerner |
| author_facet | Camilo Pérez-Sosa Ana Belén Peñaherrera-Pazmiño Gustavo Rosero Natalia Bourguignon Aparna Aravelli Shekhar Bhansali Maximiliano Sebastian Pérez Betiana Lerner |
| author_sort | Camilo Pérez-Sosa |
| collection | DOAJ |
| description | Conventional manufacturing methods for polydimethylsiloxane (PDMS)-based microdevices require multiple steps and elements that increase cost and production time. Also, these PDMS microdevices are mostly limited to single use, and it is difficult to recover the contents inside the microchannels or perform advanced microscopy visualization due to their irreversible sealing method. Herein, we developed a novel manufacturing method based on polymethylmethacrylate (PMMA) plates adjusted using a mechanical pressure-based system. One conformation of the PMMA plate assembly system allows the reproducible manufacture of PDMS replicas, reducing the cost since a precise amount of PDMS is used, and the PDMS replicas show uniform dimensions. A second form of assembling the PMMA plates permits pressure-based sealing of the PDMS layer with a glass base. By reversibly sealing the microdevice without using plasma for bonding, we achieve chip on/off configurations, which allow the user to open and close the device and reuse it in an easy-to-use way. No deformation was observed on the structures of the PDMS microchannels when a range of 10 to 18 kPa pressure was applied using the technique. Furthermore, the functionality of the proposed system was successfully validated by the generation of microdroplets with reused microdevices via three repetitions. |
| first_indexed | 2024-03-10T03:25:14Z |
| format | Article |
| id | doaj.art-959c565e0c4f4edabefb989a9234a7e2 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T03:25:14Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-959c565e0c4f4edabefb989a9234a7e22023-11-23T12:11:03ZengMDPI AGMicromachines2072-666X2022-04-0113565010.3390/mi13050650Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic DevicesCamilo Pérez-Sosa0Ana Belén Peñaherrera-Pazmiño1Gustavo Rosero2Natalia Bourguignon3Aparna Aravelli4Shekhar Bhansali5Maximiliano Sebastian Pérez6Betiana Lerner7IREN Center, National Technological University, Buenos Aires B1706EAH, ArgentinaIREN Center, National Technological University, Buenos Aires B1706EAH, ArgentinaIREN Center, National Technological University, Buenos Aires B1706EAH, ArgentinaIREN Center, National Technological University, Buenos Aires B1706EAH, ArgentinaApplied Research Center, Florida International University, Miami, FL 33174, USADepartment of Electrical and Computer Engineering, Florida International University, Miami, FL 33174, USAIREN Center, National Technological University, Buenos Aires B1706EAH, ArgentinaIREN Center, National Technological University, Buenos Aires B1706EAH, ArgentinaConventional manufacturing methods for polydimethylsiloxane (PDMS)-based microdevices require multiple steps and elements that increase cost and production time. Also, these PDMS microdevices are mostly limited to single use, and it is difficult to recover the contents inside the microchannels or perform advanced microscopy visualization due to their irreversible sealing method. Herein, we developed a novel manufacturing method based on polymethylmethacrylate (PMMA) plates adjusted using a mechanical pressure-based system. One conformation of the PMMA plate assembly system allows the reproducible manufacture of PDMS replicas, reducing the cost since a precise amount of PDMS is used, and the PDMS replicas show uniform dimensions. A second form of assembling the PMMA plates permits pressure-based sealing of the PDMS layer with a glass base. By reversibly sealing the microdevice without using plasma for bonding, we achieve chip on/off configurations, which allow the user to open and close the device and reuse it in an easy-to-use way. No deformation was observed on the structures of the PDMS microchannels when a range of 10 to 18 kPa pressure was applied using the technique. Furthermore, the functionality of the proposed system was successfully validated by the generation of microdroplets with reused microdevices via three repetitions.https://www.mdpi.com/2072-666X/13/5/650reusable microdevicereversible sealingPMMA platesmicrodroplets |
| spellingShingle | Camilo Pérez-Sosa Ana Belén Peñaherrera-Pazmiño Gustavo Rosero Natalia Bourguignon Aparna Aravelli Shekhar Bhansali Maximiliano Sebastian Pérez Betiana Lerner Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices Micromachines reusable microdevice reversible sealing PMMA plates microdroplets |
| title | Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices |
| title_full | Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices |
| title_fullStr | Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices |
| title_full_unstemmed | Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices |
| title_short | Novel Reproducible Manufacturing and Reversible Sealing Method for Microfluidic Devices |
| title_sort | novel reproducible manufacturing and reversible sealing method for microfluidic devices |
| topic | reusable microdevice reversible sealing PMMA plates microdroplets |
| url | https://www.mdpi.com/2072-666X/13/5/650 |
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