Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish.
The anion exchanger (AE) plays critical roles in physiological processes including CO2 transport and volume regulation in erythrocytes and acid-base regulation in renal tubules. Although expression of the AE in inner-ear hair cells was reported, its specific localization and function are still uncle...
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| Format: | Article |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4332475?pdf=render |
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| author | Yuan-Hsiang Lin Giun-Yi Hung Liang-Chun Wu Sheng-Wen Chen Li-Yih Lin Jiun-Lin Horng |
| author_facet | Yuan-Hsiang Lin Giun-Yi Hung Liang-Chun Wu Sheng-Wen Chen Li-Yih Lin Jiun-Lin Horng |
| author_sort | Yuan-Hsiang Lin |
| collection | DOAJ |
| description | The anion exchanger (AE) plays critical roles in physiological processes including CO2 transport and volume regulation in erythrocytes and acid-base regulation in renal tubules. Although expression of the AE in inner-ear hair cells was reported, its specific localization and function are still unclear. Using in situ hybridization, we found that the AE1b transcript is expressed in lateral-line hair cells of zebrafish larvae. An immunohistochemical analysis with a zebrafish-specific antibody localized AE1b to stereocilia of hair cells, and the expression was eliminated by morpholino knockdown of AE1b. A non-invasive, scanning ion-selective electrode technique was applied to analyze mechanotransducer (MET) channel-mediated Ca2+ influx at stereocilia of hair cells of intact fish. Ca2+ influx was effectively suppressed by AE1b morpholino knockdown and inhibitor (DIDS) treatment. Elevating external Ca2+ (0.2 to 2 mM) neutralized the inhibition of DIDS. Taken together, this study provides solid evidence to show that AE1b in stereocilia is required for the proper functioning of MET channels. |
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| id | doaj.art-95a13998bc104a499c7b946e91943f29 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:42:53Z |
| publishDate | 2015-01-01 |
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| spelling | doaj.art-95a13998bc104a499c7b946e91943f292022-12-22T01:55:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011704110.1371/journal.pone.0117041Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish.Yuan-Hsiang LinGiun-Yi HungLiang-Chun WuSheng-Wen ChenLi-Yih LinJiun-Lin HorngThe anion exchanger (AE) plays critical roles in physiological processes including CO2 transport and volume regulation in erythrocytes and acid-base regulation in renal tubules. Although expression of the AE in inner-ear hair cells was reported, its specific localization and function are still unclear. Using in situ hybridization, we found that the AE1b transcript is expressed in lateral-line hair cells of zebrafish larvae. An immunohistochemical analysis with a zebrafish-specific antibody localized AE1b to stereocilia of hair cells, and the expression was eliminated by morpholino knockdown of AE1b. A non-invasive, scanning ion-selective electrode technique was applied to analyze mechanotransducer (MET) channel-mediated Ca2+ influx at stereocilia of hair cells of intact fish. Ca2+ influx was effectively suppressed by AE1b morpholino knockdown and inhibitor (DIDS) treatment. Elevating external Ca2+ (0.2 to 2 mM) neutralized the inhibition of DIDS. Taken together, this study provides solid evidence to show that AE1b in stereocilia is required for the proper functioning of MET channels.http://europepmc.org/articles/PMC4332475?pdf=render |
| spellingShingle | Yuan-Hsiang Lin Giun-Yi Hung Liang-Chun Wu Sheng-Wen Chen Li-Yih Lin Jiun-Lin Horng Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. PLoS ONE |
| title | Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. |
| title_full | Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. |
| title_fullStr | Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. |
| title_full_unstemmed | Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. |
| title_short | Anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral-line hair cells of zebrafish. |
| title_sort | anion exchanger 1b in stereocilia is required for the functioning of mechanotransducer channels in lateral line hair cells of zebrafish |
| url | http://europepmc.org/articles/PMC4332475?pdf=render |
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