First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria
Background: Malaria is a major public health problem and life threatening parasitic vector-borne disease. For the first time, we established and report the molecular mechanism responsible for Anopheles gambiae s.l. resistance to pyrethroids and DDT from Yamaltu Deba, Southern Guinea Savanna, Norther...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-07-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/1349 |
| _version_ | 1818926243358179328 |
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| author | Mustapha Ahmed-Yusuf Hassan Vatandoost Mohammad Ali Oshaghi Ahmad Ali Hanafi-Bojd Ahmad Ali Enayati Rabiu Ibrahim Jalo |
| author_facet | Mustapha Ahmed-Yusuf Hassan Vatandoost Mohammad Ali Oshaghi Ahmad Ali Hanafi-Bojd Ahmad Ali Enayati Rabiu Ibrahim Jalo |
| author_sort | Mustapha Ahmed-Yusuf |
| collection | DOAJ |
| description | Background: Malaria is a major public health problem and life threatening parasitic vector-borne disease. For the first time, we established and report the molecular mechanism responsible for Anopheles gambiae s.l. resistance to pyrethroids and DDT from Yamaltu Deba, Southern Guinea Savanna, Northern-Nigeria.
Methods: The susceptibility profile of An. gambiae s.l. to four insecticides (DDT 4%, bendiocarb 0.1%, malathion 5% and deltamethrin 0.05%) using 2–3 days old females from larvae collected from study area between August and November, 2018 was first established. Genomic DNA was then extracted from 318 mosquitoes using Livak DNA extraction protocol for specie identification and kdr genotyping. The mosquitoes were identified to species level and then 96 genotyped for L1014F and L1014S kdr target site mutations.
Results: The mosquitoes were all resistant to DDT, bendiocarb and deltamethrin but fully susceptible to malathion. An. coluzzii was found to be the dominant sibling species (97.8%) followed by An. arabiensis (1.9%) and An. gambiae s.s (0.3%). The frequency of the L1014F kdr mutation was relatively higher (83.3%) than the L1014S (39%) in the three species studied. The L1014F showed a genotypic frequency of 75% resistance (RR), 17% heterozygous (RS) and 8% susceptible (SS) with an allelic frequency of 87% RR and 13% SS while the L1014S showed a genotypic frequency of RR (16%), RS (38%) and SS (46%) with an allelic frequency of 40% RR and 60% SS, respectively.
Conclusion: This study reveals that both kdr mutations present simultaneously in Northern-Nigeria, however contribution of L1014F which is common in West Africa was more than twice of L1014S mutation found in East Africa. |
| first_indexed | 2024-12-20T02:54:01Z |
| format | Article |
| id | doaj.art-95a14992c576480ea000a075679aca7f |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-20T02:54:01Z |
| publishDate | 2020-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-95a14992c576480ea000a075679aca7f2022-12-21T19:55:57ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712020-07-0114310.18502/jad.v14i3.45561349First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-NigeriaMustapha Ahmed-Yusuf0Hassan Vatandoost1Mohammad Ali Oshaghi2Ahmad Ali Hanafi-Bojd3Ahmad Ali Enayati4Rabiu Ibrahim Jalo5Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University, Kano, NigeriaDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology, School of Public Health and Health Sciences Research Centre, Mazandaran University of Medical Sciences, Sari, IranDepartment of Community Medicine, Bayero University, Kano, NigeriaBackground: Malaria is a major public health problem and life threatening parasitic vector-borne disease. For the first time, we established and report the molecular mechanism responsible for Anopheles gambiae s.l. resistance to pyrethroids and DDT from Yamaltu Deba, Southern Guinea Savanna, Northern-Nigeria. Methods: The susceptibility profile of An. gambiae s.l. to four insecticides (DDT 4%, bendiocarb 0.1%, malathion 5% and deltamethrin 0.05%) using 2–3 days old females from larvae collected from study area between August and November, 2018 was first established. Genomic DNA was then extracted from 318 mosquitoes using Livak DNA extraction protocol for specie identification and kdr genotyping. The mosquitoes were identified to species level and then 96 genotyped for L1014F and L1014S kdr target site mutations. Results: The mosquitoes were all resistant to DDT, bendiocarb and deltamethrin but fully susceptible to malathion. An. coluzzii was found to be the dominant sibling species (97.8%) followed by An. arabiensis (1.9%) and An. gambiae s.s (0.3%). The frequency of the L1014F kdr mutation was relatively higher (83.3%) than the L1014S (39%) in the three species studied. The L1014F showed a genotypic frequency of 75% resistance (RR), 17% heterozygous (RS) and 8% susceptible (SS) with an allelic frequency of 87% RR and 13% SS while the L1014S showed a genotypic frequency of RR (16%), RS (38%) and SS (46%) with an allelic frequency of 40% RR and 60% SS, respectively. Conclusion: This study reveals that both kdr mutations present simultaneously in Northern-Nigeria, however contribution of L1014F which is common in West Africa was more than twice of L1014S mutation found in East Africa.https://jad.tums.ac.ir/index.php/jad/article/view/1349Anopheles gambiae s.l.; Insecticide resistance; Northern Nigeria; Voltage-gated sodium channels (VGSC) |
| spellingShingle | Mustapha Ahmed-Yusuf Hassan Vatandoost Mohammad Ali Oshaghi Ahmad Ali Hanafi-Bojd Ahmad Ali Enayati Rabiu Ibrahim Jalo First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria Journal of Arthropod-Borne Diseases Anopheles gambiae s.l.; Insecticide resistance; Northern Nigeria; Voltage-gated sodium channels (VGSC) |
| title | First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria |
| title_full | First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria |
| title_fullStr | First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria |
| title_full_unstemmed | First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria |
| title_short | First Report of Target Site Insensitivity in Pyrethroid Resistant Anopheles gambiae from Southern Guinea Savanna, Northern-Nigeria |
| title_sort | first report of target site insensitivity in pyrethroid resistant anopheles gambiae from southern guinea savanna northern nigeria |
| topic | Anopheles gambiae s.l.; Insecticide resistance; Northern Nigeria; Voltage-gated sodium channels (VGSC) |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/1349 |
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