Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma
Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of c...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2013-08-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2360 |
| _version_ | 1797260897682456576 |
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| author | Amirala Aghbali Sepideh Vosough Hosseini Abbas r Delaza Nader Kalbasi Gharavi Fatemeh Zare Shahneh Mona Orangi Ali Bandehagh Behzad Baradaran |
| author_facet | Amirala Aghbali Sepideh Vosough Hosseini Abbas r Delaza Nader Kalbasi Gharavi Fatemeh Zare Shahneh Mona Orangi Ali Bandehagh Behzad Baradaran |
| author_sort | Amirala Aghbali |
| collection | DOAJ |
| description |
Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan’s test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma.
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| first_indexed | 2024-04-24T23:32:37Z |
| format | Article |
| id | doaj.art-95a18efdcbdf4c08b8fd5df3ad415eb0 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:37Z |
| publishDate | 2013-08-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-95a18efdcbdf4c08b8fd5df3ad415eb02024-03-15T14:32:48ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2013-08-0113310.17305/bjbms.2013.2360229Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinomaAmirala Aghbali0Sepideh Vosough Hosseini1Abbas r Delaza2Nader Kalbasi Gharavi3Fatemeh Zare Shahneh4Mona Orangi5Ali Bandehagh6Behzad Baradaran7Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical SciencesDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical SciencesDrug Applied Research Center, Tabriz University of Medical SciencesDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesDrug Applied Research Center, Tabriz University of Medical SciencesDrug Applied Research Center, Tabriz University of Medical Sciences Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan’s test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2360cytotoxicityapoptosisAnticanceroral squamous cell carcinomagrape seed extract |
| spellingShingle | Amirala Aghbali Sepideh Vosough Hosseini Abbas r Delaza Nader Kalbasi Gharavi Fatemeh Zare Shahneh Mona Orangi Ali Bandehagh Behzad Baradaran Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma Biomolecules & Biomedicine cytotoxicity apoptosis Anticancer oral squamous cell carcinoma grape seed extract |
| title | Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma |
| title_full | Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma |
| title_fullStr | Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma |
| title_full_unstemmed | Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma |
| title_short | Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma |
| title_sort | induction of apoptosis by grape seed extract vitis vinifera in oral squamous cell carcinoma |
| topic | cytotoxicity apoptosis Anticancer oral squamous cell carcinoma grape seed extract |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2360 |
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