Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion
Background: Otitis media with effusion (OME) is common in the children. It is proven that pathogenic bacteria as a causative agent of middle ear effusion, however the role of fungal infection in otitis media with effusion remains unclear. Therefore, this study was conducted to assess presence of fun...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2008-12-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2022 |
| _version_ | 1818772401036460032 |
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| author | MM Jalali S Rezaie A Kousha F Saadat R Banan |
| author_facet | MM Jalali S Rezaie A Kousha F Saadat R Banan |
| author_sort | MM Jalali |
| collection | DOAJ |
| description | Background: Otitis media with effusion (OME) is common in the children. It is proven that pathogenic bacteria as a causative agent of middle ear effusion, however the role of fungal infection in otitis media with effusion remains unclear. Therefore, this study was conducted to assess presence of fungi in OME.
Methods: From January 2005 to September 2006, a number of 62 children with proven otitis media with effusion subjected to the case series study at Amiralmomenin Hospital in Rasht City, Iran. After myringotomy, middle ear effusion was collected. In 48 patients, both ears demonstrated effusion, whereas in 14 patients, only one ear had effusion. Standard mycological culture and polymerase chain reaction (PCR) assay were performed in 110 and 79 samples, respectively.
Results: The growth of fungi was observed in 9 samples (8.8%). The result of our PCR assay showed that 23 samples (29.1%) were positive for fungal DNA.
Conclusion: Middle ear effusion from cases with OME contains fungi and it might play a role in the pathogens of OME. PCR assay is a better indicator in detection of fungus in middle ear effusion, compared with fungal culturing method. However, the estimation of its sensitivity and specificity in detection of fungal agents in these patients needs more molecular epidemiological studies. |
| first_indexed | 2024-12-18T10:08:46Z |
| format | Article |
| id | doaj.art-95a509c4e1c44722ab9f11faf1d9d2f7 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-18T10:08:46Z |
| publishDate | 2008-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-95a509c4e1c44722ab9f11faf1d9d2f72022-12-21T21:11:30ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932008-12-01374Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with EffusionMM Jalali 0S Rezaie 1A Kousha 2F Saadat 3R Banan 4ENT-HNS Research Center, Guilan University of Medical Sciences, IranDept. of Mycology, School of Public Health, Tehran University of Medical Sciences, IranENT-HNS Research Center, Guilan University of Medical Sciences, IranSchool of Medicine, Guilan University of Medical Sciences, IranENT-HNS Research Center, Guilan University of Medical Sciences, IranBackground: Otitis media with effusion (OME) is common in the children. It is proven that pathogenic bacteria as a causative agent of middle ear effusion, however the role of fungal infection in otitis media with effusion remains unclear. Therefore, this study was conducted to assess presence of fungi in OME. Methods: From January 2005 to September 2006, a number of 62 children with proven otitis media with effusion subjected to the case series study at Amiralmomenin Hospital in Rasht City, Iran. After myringotomy, middle ear effusion was collected. In 48 patients, both ears demonstrated effusion, whereas in 14 patients, only one ear had effusion. Standard mycological culture and polymerase chain reaction (PCR) assay were performed in 110 and 79 samples, respectively. Results: The growth of fungi was observed in 9 samples (8.8%). The result of our PCR assay showed that 23 samples (29.1%) were positive for fungal DNA. Conclusion: Middle ear effusion from cases with OME contains fungi and it might play a role in the pathogens of OME. PCR assay is a better indicator in detection of fungus in middle ear effusion, compared with fungal culturing method. However, the estimation of its sensitivity and specificity in detection of fungal agents in these patients needs more molecular epidemiological studies.https://ijph.tums.ac.ir/index.php/ijph/article/view/2022Otitis media with effusionPCRFungiIran |
| spellingShingle | MM Jalali S Rezaie A Kousha F Saadat R Banan Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion Iranian Journal of Public Health Otitis media with effusion PCR Fungi Iran |
| title | Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion |
| title_full | Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion |
| title_fullStr | Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion |
| title_full_unstemmed | Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion |
| title_short | Detection of Fungal DNA in the Middle Ear Effusion of Patients Suffering from Otitis Media with Effusion |
| title_sort | detection of fungal dna in the middle ear effusion of patients suffering from otitis media with effusion |
| topic | Otitis media with effusion PCR Fungi Iran |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2022 |
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