Apoptosis Quantification in Tissue: Development of a Semi-Automatic Protocol and Assessment of Critical Steps of Image Processing

Apoptosis Quantification in Tissue: Development of a Semi-Automatic Protocol and Assessment of Critical Steps of Image Processing

Apoptosis is associated with numerous phenotypical characteristics, and is thus studied with many tools. In this study, we compared two broadly used apoptotic assays: TUNEL and staining with an antibody targeting the activated form of an effector caspase. To compare them, we developed a protocol bas...

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Bibliographic Details
Main Authors: Juliette de Noiron, Marion Hoareau, Jessie Colin, Isabelle Guénal
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Biomolecules
Subjects:
apoptosis
TUNEL
caspase
image processing
thresholding
signal quantification
Online Access:https://www.mdpi.com/2218-273X/11/10/1523

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