Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI
Glycogen plays essential roles in glucose metabolism. Imaging glycogen in the liver, the major glycogen reservoir in the body, may shed new light on many metabolic disorders. 13C magnetic resonance spectroscopy (MRS) has become the mainstream method for monitoring glycogen in the body. However, the...
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| Format: | Article |
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KeAi Communications Co. Ltd.
2023-07-01
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| Series: | Fundamental Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266732582200423X |
| _version_ | 1797774758471794688 |
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| author | Shizhen Chen Mou Jiang Yaping Yuan Baolong Wang Yu Li Lei Zhang Zhong-Xing Jiang Chaohui Ye Xin Zhou |
| author_facet | Shizhen Chen Mou Jiang Yaping Yuan Baolong Wang Yu Li Lei Zhang Zhong-Xing Jiang Chaohui Ye Xin Zhou |
| author_sort | Shizhen Chen |
| collection | DOAJ |
| description | Glycogen plays essential roles in glucose metabolism. Imaging glycogen in the liver, the major glycogen reservoir in the body, may shed new light on many metabolic disorders. 13C magnetic resonance spectroscopy (MRS) has become the mainstream method for monitoring glycogen in the body. However, the equipment of special hardware to standard clinical magnetic resonance imaging (MRI) scanners limits its clinical applications. Herein, we utilized endogenous glycogen as a T2-based relaxation contrast agent for imaging glycogen metabolism in the liver in vivo. The in vitro results demonstrated that the transverse relaxation rate of glycogen strongly correlates with the concentration, pH, and field strength. Based on the Swift-Connick theory, we characterized the exchange property of glycogen and measured the exchange rate of glycogen as 31,847 Hz at 37 °C. Besides, the viscosity and echo spacing showed no apparent effect on the transverse relaxation rate. This unique feature enables visualization of glycogen signaling in vivo through T2-weighted MRI. Two hours-post intraperitoneal injection of glucagon, a clinical drug to promote glycogenolysis and gluconeogenesis, the signal intensity of the mice's liver increased by 1.8 times from the T2-weighted imaging experiment due to the decomposition of glycogen. This study provides a convenient imaging strategy to non-invasively investigate glycogen metabolism in the liver, which may find clinical applications in metabolic diseases. |
| first_indexed | 2024-03-12T22:25:52Z |
| format | Article |
| id | doaj.art-95a71cb8e29f44f3a678d2e2d34bcc5c |
| institution | Directory Open Access Journal |
| issn | 2667-3258 |
| language | English |
| last_indexed | 2024-03-12T22:25:52Z |
| publishDate | 2023-07-01 |
| publisher | KeAi Communications Co. Ltd. |
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| series | Fundamental Research |
| spelling | doaj.art-95a71cb8e29f44f3a678d2e2d34bcc5c2023-07-22T04:53:06ZengKeAi Communications Co. Ltd.Fundamental Research2667-32582023-07-0134481487Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRIShizhen Chen0Mou Jiang1Yaping Yuan2Baolong Wang3Yu Li4Lei Zhang5Zhong-Xing Jiang6Chaohui Ye7Xin Zhou8State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding author.Glycogen plays essential roles in glucose metabolism. Imaging glycogen in the liver, the major glycogen reservoir in the body, may shed new light on many metabolic disorders. 13C magnetic resonance spectroscopy (MRS) has become the mainstream method for monitoring glycogen in the body. However, the equipment of special hardware to standard clinical magnetic resonance imaging (MRI) scanners limits its clinical applications. Herein, we utilized endogenous glycogen as a T2-based relaxation contrast agent for imaging glycogen metabolism in the liver in vivo. The in vitro results demonstrated that the transverse relaxation rate of glycogen strongly correlates with the concentration, pH, and field strength. Based on the Swift-Connick theory, we characterized the exchange property of glycogen and measured the exchange rate of glycogen as 31,847 Hz at 37 °C. Besides, the viscosity and echo spacing showed no apparent effect on the transverse relaxation rate. This unique feature enables visualization of glycogen signaling in vivo through T2-weighted MRI. Two hours-post intraperitoneal injection of glucagon, a clinical drug to promote glycogenolysis and gluconeogenesis, the signal intensity of the mice's liver increased by 1.8 times from the T2-weighted imaging experiment due to the decomposition of glycogen. This study provides a convenient imaging strategy to non-invasively investigate glycogen metabolism in the liver, which may find clinical applications in metabolic diseases.http://www.sciencedirect.com/science/article/pii/S266732582200423XGlycogenMagnetic resonance imagingLiverT2-weighted imagingTransverse relaxation |
| spellingShingle | Shizhen Chen Mou Jiang Yaping Yuan Baolong Wang Yu Li Lei Zhang Zhong-Xing Jiang Chaohui Ye Xin Zhou Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI Fundamental Research Glycogen Magnetic resonance imaging Liver T2-weighted imaging Transverse relaxation |
| title | Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI |
| title_full | Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI |
| title_fullStr | Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI |
| title_full_unstemmed | Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI |
| title_short | Using endogenous glycogen as relaxation agent for imaging liver metabolism by MRI |
| title_sort | using endogenous glycogen as relaxation agent for imaging liver metabolism by mri |
| topic | Glycogen Magnetic resonance imaging Liver T2-weighted imaging Transverse relaxation |
| url | http://www.sciencedirect.com/science/article/pii/S266732582200423X |
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