Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming
IntroductionGloriosa superba L. is an endangered ornamental plant of significant medicinal, commercial, and cultural value. This study establishes an efficient protocol for rapid in vitro propagation of this plant species through callus-mediated organogenesis using non-dormant corm explants.MethodsP...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Horticulture |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fhort.2024.1378098/full |
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| author | Dexter Achu Mosoh Dexter Achu Mosoh Ashok Kumar Khandel Sandeep Kumar Verma Wagner A. Vendrame |
| author_facet | Dexter Achu Mosoh Dexter Achu Mosoh Ashok Kumar Khandel Sandeep Kumar Verma Wagner A. Vendrame |
| author_sort | Dexter Achu Mosoh |
| collection | DOAJ |
| description | IntroductionGloriosa superba L. is an endangered ornamental plant of significant medicinal, commercial, and cultural value. This study establishes an efficient protocol for rapid in vitro propagation of this plant species through callus-mediated organogenesis using non-dormant corm explants.MethodsPlant tissue culture techniques were employed to facilitate the in vitro regeneration process of Gloriosa superba L. This encompassed various stages, including the acquisition of plant material, surface sterilization, formulation of growth media, and the execution of callogenesis, shooting, rooting, and acclimatization experiments.Results and DiscussionInvestigation into the impact of plant growth regulators on callogenesis unveiled diverse callus morphologies, dependent on regulator type and concentration, with the NAA and KN combination emerging as the most effective for callus induction. Specifically, the callus induction medium supplemented with 1.5 mg L−1 NAA, 0.5 mg L−1 KN, and 10 mg L−1 casein hydrolysate (CH) achieved remarkable results, boasting an 81.25% callus induction rate and a substantial callus biomass fresh weight of 333 mg. Furthermore, the combination of BAP and NAA facilitated optimal shoot primordia induction and shooting in callus derived from non-dormant corm explants. The shoot induction medium, enriched with 2.0 mg L−1 BAP, 0.5 mg L−1 NAA, 5 mg L−1 CH, and 20% (v/v) coconut water (CW), resulted in an impressive 83.33% shoot primordia formation rate and an average of 6.86 shoot primordia per callus. For root induction, the medium supplemented with 1.0 mg L−1 IBA displayed a high root induction rate of 81.25% and robust rooting (+++). Successful acclimatization of in vitro plantlets in controlled environments and shade net houses culminated in thriving growth, and upon transplantation into a garden soil blend (garden soil : sand : vermiculite, 2:1:1, v/v), with direct sunlight exposure, the plantlets demonstrated commendable development and form, boasting a 69% survival rate at the seven-week mark. These findings offer a robust foundation for scalable and sustainable propagation strategies, ensuring the conservation and utilization of this valuable species. |
| first_indexed | 2024-04-24T22:56:08Z |
| format | Article |
| id | doaj.art-95a83c2478cc4deba32857f3da07f0b8 |
| institution | Directory Open Access Journal |
| issn | 2813-3595 |
| language | English |
| last_indexed | 2024-04-24T22:56:08Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Horticulture |
| spelling | doaj.art-95a83c2478cc4deba32857f3da07f0b82024-03-18T04:55:46ZengFrontiers Media S.A.Frontiers in Horticulture2813-35952024-03-01310.3389/fhort.2024.13780981378098Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media primingDexter Achu Mosoh0Dexter Achu Mosoh1Ashok Kumar Khandel2Sandeep Kumar Verma3Wagner A. Vendrame4Centre for Biodiversity Exploration and Conservation (CBEC), Jabalpur, MP, IndiaSchool of Sciences, Sanjeev Agrawal Global Educational (SAGE) University, Bhopal, MP, IndiaCentre of Excellence in Research & Entrepreneurship Development, Bambooram Agro PVT. LTD., Sehore, MP, IndiaInstitute of Biological Science, Sanjeev Agrawal Global Educational (SAGE) University, Indore, MP, IndiaEnvironmental Horticulture Department, University of Florida, Institute of Food and Agricultural Sciences, 2043 IFAS Research Dr., Gainesville, FL, United StatesIntroductionGloriosa superba L. is an endangered ornamental plant of significant medicinal, commercial, and cultural value. This study establishes an efficient protocol for rapid in vitro propagation of this plant species through callus-mediated organogenesis using non-dormant corm explants.MethodsPlant tissue culture techniques were employed to facilitate the in vitro regeneration process of Gloriosa superba L. This encompassed various stages, including the acquisition of plant material, surface sterilization, formulation of growth media, and the execution of callogenesis, shooting, rooting, and acclimatization experiments.Results and DiscussionInvestigation into the impact of plant growth regulators on callogenesis unveiled diverse callus morphologies, dependent on regulator type and concentration, with the NAA and KN combination emerging as the most effective for callus induction. Specifically, the callus induction medium supplemented with 1.5 mg L−1 NAA, 0.5 mg L−1 KN, and 10 mg L−1 casein hydrolysate (CH) achieved remarkable results, boasting an 81.25% callus induction rate and a substantial callus biomass fresh weight of 333 mg. Furthermore, the combination of BAP and NAA facilitated optimal shoot primordia induction and shooting in callus derived from non-dormant corm explants. The shoot induction medium, enriched with 2.0 mg L−1 BAP, 0.5 mg L−1 NAA, 5 mg L−1 CH, and 20% (v/v) coconut water (CW), resulted in an impressive 83.33% shoot primordia formation rate and an average of 6.86 shoot primordia per callus. For root induction, the medium supplemented with 1.0 mg L−1 IBA displayed a high root induction rate of 81.25% and robust rooting (+++). Successful acclimatization of in vitro plantlets in controlled environments and shade net houses culminated in thriving growth, and upon transplantation into a garden soil blend (garden soil : sand : vermiculite, 2:1:1, v/v), with direct sunlight exposure, the plantlets demonstrated commendable development and form, boasting a 69% survival rate at the seven-week mark. These findings offer a robust foundation for scalable and sustainable propagation strategies, ensuring the conservation and utilization of this valuable species.https://www.frontiersin.org/articles/10.3389/fhort.2024.1378098/fullacclimatizationcallogenesisgermplasm conservationfloriculturein vitro mass propagationornamental plants |
| spellingShingle | Dexter Achu Mosoh Dexter Achu Mosoh Ashok Kumar Khandel Sandeep Kumar Verma Wagner A. Vendrame Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming Frontiers in Horticulture acclimatization callogenesis germplasm conservation floriculture in vitro mass propagation ornamental plants |
| title | Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming |
| title_full | Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming |
| title_fullStr | Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming |
| title_full_unstemmed | Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming |
| title_short | Optimizing callus induction and indirect organogenesis in non-dormant corm explants of Gloriosa superba (L.) via media priming |
| title_sort | optimizing callus induction and indirect organogenesis in non dormant corm explants of gloriosa superba l via media priming |
| topic | acclimatization callogenesis germplasm conservation floriculture in vitro mass propagation ornamental plants |
| url | https://www.frontiersin.org/articles/10.3389/fhort.2024.1378098/full |
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